1.
Methods Enzymol
; 594: 123-138, 2017.
Article
in English
| MEDLINE
| ID: mdl-28779838
ABSTRACT
Here, we present a simple overexpression condition for high-throughput screening of membrane proteins in Escherichia coli. For the vast majority of bacterial membrane protein targets tested the MEMbrane protein Single shoT Amplification Recipe-MemStar-leads to high production yields of target protein. The use of MemStar has facilitated structural studies of several transport proteins.