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1.
ACS Synth Biol ; 4(1): 48-56, 2015 Jan 16.
Article in English | MEDLINE | ID: mdl-24877739

ABSTRACT

RNA replicons are an emerging platform for engineering synthetic biological systems. Replicons self-amplify, can provide persistent high-level expression of proteins even from a small initial dose, and, unlike DNA vectors, pose minimal risk of chromosomal integration. However, no quantitative model sufficient for engineering levels of protein expression from such replicon systems currently exists. Here, we aim to enable the engineering of multigene expression from more than one species of replicon by creating a computational model based on our experimental observations of the expression dynamics in single- and multireplicon systems. To this end, we studied fluorescent protein expression in baby hamster kidney (BHK-21) cells using a replicon derived from Sindbis virus (SINV). We characterized expression dynamics for this platform based on the dose-response of a single species of replicon over 50 h and on a titration of two cotransfected replicons expressing different fluorescent proteins. From this data, we derive a quantitative model of multireplicon expression and validate it by designing a variety of three-replicon systems, with profiles that match desired expression levels. We achieved a mean error of 1.7-fold on a 1000-fold range, thus demonstrating how our model can be applied to precisely control expression levels of each Sindbis replicon species in a system.


Subject(s)
Genetic Engineering/methods , Models, Genetic , Animals , Cell Line , Cricetinae , Gene Expression , Luminescent Proteins/genetics , RNA, Viral/genetics , Replicon , Sindbis Virus/genetics , Synthetic Biology , Transfection
2.
Virology ; 441(1): 12-7, 2013 Jun 20.
Article in English | MEDLINE | ID: mdl-23608360

ABSTRACT

The nucleocapsids of many plant viruses are significantly more robust and protective of their RNA contents than those of enveloped animal viruses. In particular, the capsid protein (CP) of the plant virus Cowpea Chlorotic Mottle Virus (CCMV) is of special interest because it has been shown to spontaneously package, with high efficiency, a large range of lengths and sequences of single-stranded RNA molecules. In this work we demonstrate that hybrid virus-like particles, assembled in vitro from CCMV CP and a heterologous RNA derived from a mammalian virus (Sindbis), are capable of releasing their RNA in the cytoplasm of mammalian cells. This result establishes the first step in the use of plant viral capsids as vectors for gene delivery and expression in mammalian cells. Furthermore, the CCMV capsid protects the packaged RNA against nuclease degradation and serves as a robust external scaffold with many possibilities for further functionalization and cell targeting.


Subject(s)
Bromovirus/genetics , Capsid/metabolism , Gene Transfer Techniques , Gene Transfer, Horizontal , RNA/metabolism , Animals , Cell Line , Cricetinae , RNA/genetics , Sindbis Virus/genetics , Virus Assembly
3.
J Virol ; 85(12): 5773-81, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21471237

ABSTRACT

Alphaviruses are small, spherical, enveloped, positive-sense, single-stranded, RNA viruses responsible for considerable human and animal disease. Using microinjection of preassembled cores as a tool, a system has been established to study the assembly and budding process of Sindbis virus, the type member of the alphaviruses. We demonstrate the release of infectious virus-like particles from cells expressing Sindbis virus envelope glycoproteins following microinjection of Sindbis virus nucleocapsids purified from the cytoplasm of infected cells. Furthermore, it is shown that nucleocapsids assembled in vitro mimic those isolated in the cytoplasm of infected cells with respect to their ability to be incorporated into enveloped virions following microinjection. This system allows for the study of the alphavirus budding process independent of an authentic infection and provides a platform to study viral and host requirements for budding.


Subject(s)
Kidney/virology , Nucleocapsid/administration & dosage , Virion/physiology , Virus Assembly/physiology , Virus Release/physiology , Alphavirus/genetics , Alphavirus/metabolism , Alphavirus/physiology , Animals , Cell Line , Cricetinae , Humans , Kidney/cytology , Microinjections , Nucleocapsid/genetics , Nucleocapsid/isolation & purification , Nucleocapsid/metabolism , Sindbis Virus/metabolism , Sindbis Virus/physiology , Viral Envelope Proteins/genetics , Viral Envelope Proteins/metabolism , Virion/pathogenicity , Virology/methods
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