Advantages of one step nucleic acid amplification (OSNA) whole node assay in sentinel lymph node (SLN) analysis in breast cancer.

BACKGROUND: The purpose of this study is to present our first results of sentinel node analysis (SLN) by one step nucleic acid amplification (OSNA) in routine clinical practice in our centre and compare them with the results of classic histopathological analysis in a historical cohort from our same institution. METHODS: 407 patients (total study population) with early breast cancer and no clinical nodal involvement underwent SLN biopsy in our institution. The SLN was analysed by OSNA in 164 biopsies. OSNA results were compared with the conventional histopathology study of 244 patients who had undergone SLN biopsy previously. The characteristics of the patients in both groups were evaluated and a comparison was made of the rate of metastases detected by both methods and of the surgical procedures needed in each group. We also investigated the state of non-sentinel lymph nodes if micrometastases where found in SLN. RESULTS: SLN biopsy result was considered as positive in 45 patients (28%) in the OSNA group and in 58 in the historical group (24%). There was no difference in the rate of macrometastases (16,5% for OSNA, 20% for HE) but we found differences in the rate of micrometastases (11% for OSNA and 3,6% for HE p = 0.0007). Axillary lymphadenectomy (ALND) was performed in 43/45 cases in the OSNA group and in 51/58 of the historical group. In all patients diagnosed by OSNA, ALND was performed during the initial surgical procedure. In the historical cohort ALND was performed during the initial surgical procedure in 41 patients and in a second surgical procedure in 10 patients. Patients from both groups with micrometastases in the SLN had no metastases in other nodes when the ALND was performed. CONCLUSIONS: OSNA analysis allows the detection of SLN metastases as precisely as conventional pathology with an increased detection of micrometastases. The OSNA method can reduce the need of a deferred lymphadenectomy.

Histiocitoma fibroso maligno mamarioen la mujer joven: un caso excepcional / Malignant fibrous histiocytoma of the breast: an exceptional case

Los sarcomas primarios de mama son procesos patológicos poco frecuentes. En concreto, el histiocitoma fibroso maligno raramente se observa como tumor mamario. Afecta preferentemente a mujeres de 40 a 60 años y es de difícil diagnóstico. Clínicamente suele presentarse como una tumoración en la mama que acostumbra a ser de gran tamaño (5-10 cm). El tratamiento consiste en la exéresis amplia con márgenes libres sin linfadenectomía. El caso que presentamos resulta interesante por la infrecuencia tumoral, la edad temprana de presentación y la dificultad diagnóstica que plantea en la mujer joven. (AU)