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1.
Sci Rep ; 6: 37322, 2016 12 08.
Article in English | MEDLINE | ID: mdl-27929032

ABSTRACT

Phytoecdysteroids like 20-hydroxyecdysone ("ecdysterone") can exert a mild, non-hormonal anabolic/adaptogenic activity in mammals, and as such, are frequently used in food supplements. Spinach is well-known for its relatively low ecdysteroid content. Cyanotis arachnoidea, a plant native in China, is among the richest sources of phytoecdysteroids, and extracts of this plant are marketed in tons per year amounts via the internet at highly competitive prices. Here we report the investigation of a series of food supplements produced in Germany and claimed to contain spinach extracts. Twelve ecdysteroids including two new compounds were isolated and utilized as marker compounds. A comparative analysis of the products with Cyanotis and spinach extracts provides evidence that they were manufactured from Cyanotis extracts instead of spinach as stated. Based on the chromatographic fingerprints, 20-hydroxyecdysone 2- and 3-acetate are suggested as diagnostic markers for related quality control. This case appears to represent an unusual type of dietary supplement counterfeiting: undeclared extracts from alternative plants would supposedly 'guarantee' product efficacy.


Subject(s)
Commelinaceae/chemistry , Dietary Supplements/standards , Ecdysteroids/analysis , Spinacia oleracea/chemistry , Animals , China , Ecdysone/analysis , Ecdysone/isolation & purification , Ecdysteroids/isolation & purification , Ecdysterone/analysis , Ecdysterone/isolation & purification , Germany , Phytosterols/analysis , Phytosterols/isolation & purification , Plant Extracts/chemistry , Quality Control
2.
J Med Food ; 16(5): 404-9, 2013 May.
Article in English | MEDLINE | ID: mdl-23631495

ABSTRACT

Numerous honeybee products are used in medicine, but the literature furnishes no information concerning the effects of the drone milk (DM), although drone brood, which is similar to DM, was reported to elicit a hormone-like strengthening effect. In certain countries, DM is traditionally used to treat infertility and to promote vitality in both men and women. The aim of this study was to determine the putative estrogen hormone-like effect of raw DM in rats and to identify the effective compounds. Uterotrophic assays revealed that DM increased the relative weight of the immature rat uterus. This effect was confirmed by reverse transcription polymerase chain-reaction and Western blot methods, in which the mRNA and protein expression of the estrogen-dependent peptide complement component C3 was determined. Column chromatography and uterotrophic assays were used to fractionate and check bioactivity, respectively. The active compound after the last fractionation was identified by the nuclear magnetic resonance and mass spectrometry techniques as E-dec-2-enedioic acid, which is very similar to the fatty acids with estrogenic activity that were previously isolated from royal jelly. These results lead us to suppose that E-dec-2-enedioic acid is responsible for the estrogen-like effect of DM. This appears to be the first report on the pharmacological effects of DM and E-dec-2-enedioic acid in mammals.


Subject(s)
Bees/metabolism , Estrogens/administration & dosage , Fatty Acids/pharmacology , Uterus/drug effects , Animals , Bees/chemistry , Estrogens/analysis , Fatty Acids/analysis , Female , Humans , Male , Rats , Rats, Sprague-Dawley , Uterus/growth & development , Uterus/metabolism
3.
ScientificWorldJournal ; 2012: 651275, 2012.
Article in English | MEDLINE | ID: mdl-22645442

ABSTRACT

Two new and one known ecdysteroids were identified in the methanolic extract of the roots of Serratula wolffii. The new compounds isolated were ponasterone A-22-apioside (1) and 3-epi-shidasterone (3), together with the known 3-epi-22-deoxy-20-hydroxyecdysone (2). The structures of compounds 1-3 were determined by extensive spectroscopic techniques, including one- and two-dimensional NMR methods.


Subject(s)
Asteraceae/metabolism , Ecdysteroids/chemistry , Chemistry, Pharmaceutical/methods , Drug Design , Ecdysterone/analogs & derivatives , Ecdysterone/chemistry , Glycosides/chemistry , Magnetic Resonance Spectroscopy/methods , Mass Spectrometry/methods , Models, Chemical , Plant Extracts/pharmacology , Plant Roots/metabolism , Spectrophotometry/methods
4.
J Med Chem ; 55(11): 5034-43, 2012 Jun 14.
Article in English | MEDLINE | ID: mdl-22578055

ABSTRACT

Multidrug resistance (MDR) is a major cause of failure of cancer chemotherapy. Fifty-eight ecdysteroids, herbal analogues of the insect molting hormone and their semisynthetic derivatives, were tested for their activity against L5178 mouse T-cell lymphoma cells (non-MDR) and their subcell line transfected with pHa MDR1/A retrovirus overexpressing the human ABCB1 efflux pump (MDR cell line). The compounds showed very low antiproliferative activities but modulated the efflux of rhodamine 123 mediated by the ABCB1 transporter. Roughly depending on the polarity, mild to strong synergism or antagonism was observed by combining ecdysteroids with doxorubicin, and specific structure-activity relationships were also found. Our results show the effect of ecdysteroids on MDR cancer cells for the first time. Less polar derivatives may serve as valuable leads toward a potent and safe resistance modulator. Biological significance of the resistance-increasing activity of the most abundant phytoecdysteroids including 20-hydroxyecdysone is yet to be clarified.


Subject(s)
ATP Binding Cassette Transporter, Subfamily B, Member 1/metabolism , Antineoplastic Agents, Phytogenic/pharmacology , Doxorubicin/pharmacology , Drug Resistance, Neoplasm/drug effects , Ecdysteroids/pharmacology , ATP Binding Cassette Transporter, Subfamily B , Animals , Antibiotics, Antineoplastic , Antineoplastic Agents, Phytogenic/chemical synthesis , Antineoplastic Agents, Phytogenic/chemistry , Cell Line, Tumor , Drug Antagonism , Drug Resistance, Multiple/drug effects , Drug Screening Assays, Antitumor , Drug Synergism , Ecdysteroids/chemical synthesis , Ecdysteroids/chemistry , Humans , Mice , Structure-Activity Relationship
5.
Steroids ; 76(13): 1419-24, 2011 Dec 11.
Article in English | MEDLINE | ID: mdl-21803067

ABSTRACT

Three new compounds (3, 7, and 11) together with eight known phytoecdysteroids (1, 2, 4-6, and 8-10) were isolated from the rhizomes of common polypody, Polypodium vulgare L. The structures of compounds were elucidated by spectroscopic methods including 1D and 2D NMR measurements. The (1)H and (13)C NMR assignments of compounds 1, 6, 9 and 10 are included.


Subject(s)
Ecdysteroids/chemistry , Ecdysteroids/isolation & purification , Polypodium/chemistry , Magnetic Resonance Spectroscopy , Rhizome/chemistry
6.
Curr Med Chem ; 17(18): 1974-94, 2010.
Article in English | MEDLINE | ID: mdl-20377509

ABSTRACT

Phytoecdysteroids are plant steroids with identical or analogue structures to the molting hormone in arthropods. The ecdysteroids exert several beneficial effects on mammals, from which the most cited and deeply examined one is the increase of muscle size and strength. This shows similarities with the mode of action of the androgenic steroids but the ecdysteroids do not bind to the cytoplasmic/nuclear receptor of the mammalian steroids. These findings led to the hypothesis that ecdysteroids possibly bind to membrane bound receptors and they are likely to influence signal transduction pathways. Probably because of their closely related chemical structures, ecdysteroids exert some similar effects in vertebrates to those of the hormone 1 alpha,25-dihydroxyvitamin D3 (1,25D) which is produced in the kidney from 25-hydroxyvitamin D3, after being converted in the liver from Vitamin D3. 1,25D generates biological responses via both genomic and rapid, nongenomic mechanisms. Structure-activity relationship studies with different Vitamin D analogues could open the possibility to show that the two ways of action (genomic and nongenomic) can be influenced separately. The connection between the Vitamin D status and muscle function is already well-described in clinical studies, and several efforts have been made to evaluate the effect of Vitamin D deficiency or supplementation on muscle morphological changes and the underlying molecular mechanisms. This paper aims to summarize the main structural commonalities between the ecdysteroids, 1,25D and other Vitamin D analogues. The similarities in their effects and pathways that might be involved in the mechanism of action of these compounds will also be discussed.


Subject(s)
Ecdysteroids/analogs & derivatives , Ecdysteroids/metabolism , Vitamin D/analogs & derivatives , Vitamin D/metabolism , Animals , Humans , Models, Molecular
7.
Magn Reson Chem ; 48(5): 386-91, 2010 May.
Article in English | MEDLINE | ID: mdl-20205207

ABSTRACT

Three new natural ecdysteroids viz. 22-dehydro-20-deoxy-ajugasterone C (1), 1-hydroxy-22-deoxy-20,21-didehydro-ecdysone (2) and 22-deoxy-20,21-didehydro-ecdysone (3) were isolated from the methanol extract of the roots of Serratula wolffii. The structures of compounds 1-3 were established by various spectroscopic techniques, including one- and two-dimensional NMR, circular dichroism and mass spectroscopic methods.


Subject(s)
Asteraceae/chemistry , Ecdysteroids/chemistry , Plant Roots/chemistry , Circular Dichroism , Magnetic Resonance Spectroscopy , Molecular Structure , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Ultraviolet
8.
J Nat Prod ; 72(5): 929-32, 2009 May 22.
Article in English | MEDLINE | ID: mdl-19338317

ABSTRACT

Investigation of the ecdysteroid constituents of the herb Ajuga reptans var. reptans resulted in the isolation of three new ecdysteroids, named reptanslactone A (2), reptanslactone B (3), and sendreisterone (5), and the known 24-dehydroprecyasterone (1) and breviflorasterone (4). The structures of compounds 1-5 were determined by spectroscopic methods including one- and two-dimensional NMR measurements.


Subject(s)
Ajuga/chemistry , Ecdysteroids/isolation & purification , Ecdysteroids/chemistry , Hungary , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Stereoisomerism
9.
J Nat Prod ; 71(8): 1461-3, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18661952

ABSTRACT

Four new 26-hydroxylated phytoecdysteroids, 2-deoxy-5,20,26-trihydroxyecdysone (1), 5,20,26-trihydroxyecdysone 20,22-acetonide (2), 2-deoxy-5,20,26-trihydroxyecdysone 20,22-acetonide (3), and 20,26-dihydroxyecdysone 20,22-acetonide (4), were isolated from the herb Silene viridiflora, and their structures were elucidated by means of one- and two-dimensional NMR and mass spectrometry.


Subject(s)
Ecdysteroids/isolation & purification , Silene/chemistry , Ecdysteroids/chemistry , Hydroxylation , Magnetic Resonance Spectroscopy , Molecular Structure , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Ultraviolet
10.
J Chromatogr Sci ; 46(2): 111-6, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18366868

ABSTRACT

A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extraction on octadecyl silica, and crystallization). This procedure is extended using classical normal-phase liquid column chromatography, rotation planar chromatography, and preparative high-performance liquid chromatography for the isolation of the minor ecdysteroids: integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26-dihydroxyecdysone, and polypodine B. 2-Deoxy-20,26-dihydroxyecdysone is isolated from this species for the first time. The isolation of these ecdysteroids in adequate amounts makes them readily available for insect physiology experiments and for structure-activity relationship studies. The preparative-scale separation work also results in a minor, as yet unknown ecdysteroid.


Subject(s)
Chromatography, Liquid/methods , Ecdysteroids/chemistry , Ecdysteroids/isolation & purification , Ecdysterone/isolation & purification , Silene/chemistry , Ecdysteroids/analysis , Ecdysterone/analysis , Ecdysterone/chemistry , Mass Spectrometry , Nuclear Magnetic Resonance, Biomolecular
11.
Curr Med Chem ; 15(1): 75-91, 2008.
Article in English | MEDLINE | ID: mdl-18220764

ABSTRACT

Phytoecdysteroids are structural analogs of the insect molting hormone ecdysone. Plants comprise rich sources of ecdysteroids in high concentration and with broad structural diversity. Ecdysteroids have a number of proven beneficial effects on mammals but the hormonal effects of ecdysteroids have been proven only in arthropods. Their structures are somewhat similar to those of the vertebrate steroid hormones but there are several structural differences between the two steroid groups. Despite of these essential structural differences, ecdysteroids exert numerous effects in vertebrates that are similar to those of vertebrate hormonal steroids, and they may serve as effective anabolic, hepatoprotective, immunoprotective, antioxidant and hypoglycemic agents. Ecdysteroids do not bind to the cytosolic steroid receptors, instead, they are likely to influence signal transduction pathways, like the anabolic steroids, possibly via membrane bound receptors. The application of phytoecdysteroids is a promising alternative to the use of anabolic-androgenic steroids because of the apparent lack of adverse effects. The prospective use of phytoecdysteroids may extend to treatments of pathological conditions where anabolic steroids are routinely applied. One of the most cited aspects of phytoecdysteroid application (on the Internet) is the increase of muscle size. However in this field too stringent research is needed as an adequate cytological explanation is not yet available for the anabolic. This paper reports on the most important structural differences between androgenic hormones, their synthetic analogs and ecdysteroids. The anabolic/hormonal effects and the possible mechanisms of action of these compounds are also discussed as concerns the skeletal muscle.


Subject(s)
Anabolic Agents/pharmacology , Androgens/pharmacology , Ecdysone/pharmacology , Phytosterols/pharmacology , Anabolic Agents/chemistry , Anabolic Agents/therapeutic use , Androgens/chemistry , Animals , Ecdysone/chemistry , Ecdysone/therapeutic use , Humans , Molecular Structure , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Phytosterols/chemistry , Phytosterols/therapeutic use , Protein Biosynthesis/drug effects , Signal Transduction/drug effects , Structure-Activity Relationship
12.
Steroids ; 72(11-12): 751-5, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17698156

ABSTRACT

Investigation of the methanol extract of the roots of Serratula wolffii resulted in an ecdysone-related compound, 2beta,3beta,20R,22R,25-pentahydroxy-5beta-cholest-6,8(14)-dien (1), a new ecdysteroid, 24-methylene-shidasterone (2), the known compound stachysterone B (3) and its 14,15-alpha-epoxide (4), a novel natural product. The structures of compounds 1-4 were established by spectral analysis ((1)H NMR, (13)C NMR, COSY, NOESY, HMQC, HMQC-TOCSY and HMBC).


Subject(s)
Asteraceae/chemistry , Phytosterols/isolation & purification , Plant Roots/chemistry , Carbon Isotopes , Magnetic Resonance Spectroscopy , Phytosterols/chemistry , Protons
13.
Acta Pharm Hung ; 77(1): 11-8, 2007.
Article in Hungarian | MEDLINE | ID: mdl-17518108

ABSTRACT

Seven ecdysteroids have been isolated from the methanolic extract of the herb Serratula wolffii Andrae. The isolation process involved the removal of polar and apolar contaminants, and also included the separation of the target ecdysteroids from each other by using combined chromatographic methods. Isolation of the pure compounds required a minimum of 2 and a maximum of 7 consecutive chromatographic steps with different selectivity. Six of the isolated ecdysteroids showed the presence of an 11alpha-hydroxyl group on the steroidal skeleton, which structure may enhance the increasing effect of ecdysteroids on the protein synthesys of mammals, according to literature data. Ajugasterone C, one of these compounds was found to be present in remarkable amount in the plant. One ecdysteroid, 11 alpha-hydroxypoststerone was identified as a new natural compound.


Subject(s)
Asteraceae , Ecdysteroids/isolation & purification , Animals , Ecdysteroids/chemistry , Hungary , Hydroxylation , Mammals , Protein Biosynthesis/drug effects
14.
J Nat Prod ; 70(5): 884-6, 2007 May.
Article in English | MEDLINE | ID: mdl-17417908

ABSTRACT

Two new natural ecdysteroids, 20,22-didehydrotaxisterone (1) and 1-hydroxy-20,22-didehydrotaxisterone (2), were isolated from the roots of Serratula wolffii. Their structures were elucidated by 1D and 2D NMR spectroscopy and mass spectrometry. The biological activities of these compounds were determined via oral aphid (Acyrthosiphon pisum (Harris)) tests. Compound 1 was inactive and compound 2 exhibited very low toxicity in the oral aphid test. The activities of these two ecdysteroids were in agreement with those of other 22-deoxyecdysteroids.


Subject(s)
Asteraceae/chemistry , Ecdysteroids/isolation & purification , Plants, Medicinal/chemistry , Animals , Aphids/drug effects , Ecdysteroids/chemistry , Ecdysteroids/pharmacology , Hungary , Larva/drug effects , Molecular Structure , Plant Roots/chemistry
15.
J Chromatogr Sci ; 45(2): 76-86, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17425136

ABSTRACT

Numerous ecdysteroids are isolated from the herb of Serratula wolffii Andrae, a cultivated plant. The isolation procedure includes a variety of low-pressure liquid chromatography, thin-layer chromatography (TLC), gel chromatography, and high-performance liquid chromatography (HPLC) methods. The progress of separation is monitored by TLC, and the final proof of purity is carried out by HPLC. The isolation process involves the removal of proteins, flavonoids, chlorophylls, other sterines, etc. The purification also includes the separation of the target ecdysteroids from each other. Isolation of the pure compounds requires 2-8 chromatographic steps. The consecutive steps are based on the different physicochemical properties of the ecdysteroids. In some cases, a special peak-cut method employing a flush of dichloromethane into the dichloromethane-isopropanol-water mobile phase is used. This flush of dichloromethane leads to an almost perfect separation of otherwise unresolved peaks. Two ecdysteroids, 25-hydroxydacryhainansterone and 14-epi-20-hydroxyecdysone, are identified as natural products for the first time. The structure-chiroptical relationships for some ecdysteroids are also discussed.


Subject(s)
Asteraceae/chemistry , Chromatography, High Pressure Liquid/methods , Ecdysteroids/isolation & purification , Chromatography, Thin Layer , Circular Dichroism , Ecdysteroids/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Spectrophotometry, Ultraviolet
16.
Curr Med Chem ; 12(2): 153-72, 2005.
Article in English | MEDLINE | ID: mdl-15638733

ABSTRACT

An overview is given on both well-known and recently discovered phytoecdyteroids including a sophisticated isolation scheme and notable physiological and pharmacological effects of ecdysteroids on vertebrates. The isolation of pure ecdysteroids has been improved by the use of low-pressure reversed-phase chromatography. An optimized combination of preliminary purification and chromatographic separations results in pure ecdysteroids. Structural elucidation has been done using spectroscopic methods, however, the final proof of the steric structure is rendered using x-ray crystallography. Ecdysteroid containing preparations show a boom and both OTC products and numerous preparation techniques can be found using the Internet. This paper will give a review on the kaleidoscope of pharmacological effects attributed to the ecdysteroids, such as: An increase of protein synthesis (for body-building, AIDS, patients with neoplasm disease, etc.), and other body functions; Antidepressant effect; Shielding the body from stress, and improve the physical and sexual performance; Prevention from infections and certain diseases. A list of recent offers of ecdysteroid-containing products will also be given. The perspective use of ecdysteroids is promising in genetics. Steroid regulation of programmed cell death during development and differentiation has recently come to the limelight. Murine model of human diseases and its influencing with ecdysteroids are detailed.


Subject(s)
Ecdysteroids , Gene Expression/drug effects , Plants, Medicinal/chemistry , Animals , Antidepressive Agents/isolation & purification , Antidepressive Agents/pharmacology , Carbohydrate Metabolism , Ecdysteroids/chemistry , Ecdysteroids/isolation & purification , Ecdysteroids/pharmacology , Humans , Infection Control , Models, Animal , Proteins/metabolism , Structure-Activity Relationship
17.
Pest Manag Sci ; 60(11): 1099-104, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15532684

ABSTRACT

We studied the efficacy of water-soluble extracts from four Ajuga spp on the post-embryonic development of two exopterygota (sucking insect) species. To allow comparison between different Ajuga species, results are expressed in terms of quantity of plant extracted per litre of test solution. Crude methanolic extracts of all Ajuga plants tested, with the exception of A genevensis, showed considerable per os efficacy against larvae of both Dysdercus cingulatus F and Acyrthosiphon pisum (Harris) even at 1 g litre(-1). In the aphid tests the order of efficacy was A bracteosa Wallich ex Benth > A chamaepitys Schreber > A reptans L > A genevensis L. On D cingulatus the order of efficacy was: A reptans > A bracteosa > A chamaepitys > A genevensis. Extracts were fractionated on SepPak using a range of methanol/water mixtures. Results are expressed in terms of the initial weight of plant extracted. The 100% methanolic fraction of A chamaepitys was highly effective on A pisum (100% mortality at 1 g litre(-1)) and less effective on D cingulatus (about 60% mortality at 5 g litre(-1)). The entire 60 methanol + 40 water fraction was effective against test insects but showed different efficacies according to test species and concentration applied. 20-Hydroxyecdysone (20E), cyasterone (Cy) and ajugalactone (Ajl) were identified in the fractions from all Ajuga species, but the remaining phytoecdysteroid profile was quite different between Ajuga species. Capitasterone (Cap) and 28-epi-sengosterone (5Cy28') were found only in A reptans, makisterone A (MaA) and 29-norcyasterone (29NCy) were only in A chamaepitys, while 22-acetylcyasterone (Cy22A), 3-epi-cyasterone (Cy') and 3-epi-22-acetylcyasterone (Cy'22A) were only in A bracteosa. The total amount of phytoecdysteroids was 2053 mgkg(-1) for A bracteosa, 1892 mgkg(-1) for A reptans and 95 mg kg(-1) for A chamaepitys.


Subject(s)
Ajuga/chemistry , Aphids/drug effects , Ecdysteroids/pharmacology , Insecticides/pharmacology , Plant Extracts/pharmacology , Animals , Biological Assay , Dose-Response Relationship, Drug , Ecdysteroids/chemistry , Female , Insecticides/chemistry , Larva , Pest Control, Biological/methods
18.
J Nat Prod ; 67(6): 1070-2, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15217300

ABSTRACT

11alpha-Hydroxypoststerone (1) and herkesterone (2), two new natural ecdysteroids, were isolated from the herb Serratula wolffii. The former compound is the first 11-hydroxylated C21 ecdysteroid, while the latter is a new ecdysteroid with a 7,9(11)-dien-6-one chromophore. Their structures were determined using a combination of spectroscopic techniques.


Subject(s)
Asteraceae/chemistry , Ecdysteroids/isolation & purification , Ecdysteroids/chemistry , Ecdysteroids/pharmacology , Hungary , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular
19.
Steroids ; 69(6): 389-94, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15219788

ABSTRACT

A new natural ecdysteroid, 9beta,20-dihydroxyecdysone (1) and four related compounds 5alpha-20-hydroxyecdysone (2), 5alpha-2-deoxy-integristerone A (3), integristerone A (4) and 22-deoxy-integristerone A (5) were isolated from the herb of Silene italica ssp. nemoralis. Compound 1 is the C-9 epimer of the known 9alpha,20-dihydroxyecdysone (6) and represents a peculiar steroid skeleton. The structures of the compounds were elucidated by 1D and 2D NMR, IR and MS spectroscopy.


Subject(s)
Ecdysteroids/analogs & derivatives , Ecdysteroids/chemistry , Silene/chemistry , Ecdysteroids/isolation & purification , Molecular Conformation
20.
J Chromatogr Sci ; 42(5): 275-9, 2004.
Article in English | MEDLINE | ID: mdl-15189602

ABSTRACT

A suitable combination of preparative scale separation methods results in effective clean-up of the ecdysteroids of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The isolation of minor ecdysteroids from the partially purified extract is based on the use of both droplet counter-current chromatography and low-pressure reversed-phase liquid chromatography. The purification is completed by preparative thin-layer chromatography and preparative high-performance liquid chromatography to obtain the minor ecdysteroids, such as 2-deoxy-20-hydroxyecdysone, shidasterone, 2-deoxy-polypodine B, makisterone C, and 9alpha,20-dihydroxyecdysone.


Subject(s)
Ecdysone/analogs & derivatives , Ecdysone/isolation & purification , Ecdysteroids/isolation & purification , Silene/chemistry , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Countercurrent Distribution , Structure-Activity Relationship
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