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1.
Biophys J ; 121(6): 869-885, 2022 03 15.
Article in English | MEDLINE | ID: mdl-35182541

ABSTRACT

Electric phenomena in brain tissue can be measured using extracellular potentials, such as the local field potential, or the electro-encephalogram. The interpretation of these signals depends on the electric structure and properties of extracellular media, but the measurements of these electric properties are still debated. Some measurements point to a model in which the extracellular medium is purely resistive, and thus parameters such as electric conductivity and permittivity should be independent of frequency. Other measurements point to a pronounced frequency dependence of these parameters, with scaling laws that are consistent with capacitive or diffusive effects. However, these experiments correspond to different preparations, and it is unclear how to correctly compare them. Here, we provide for the first time, impedance measurements (in the 1-10 kHz frequency range) using the same setup in various preparations, from primary cell cultures to acute brain slices, and a comparison with similar measurements performed in artificial cerebrospinal fluid with no biological material. The measurements show that when the current flows across a cell membrane, the frequency dependence of the macroscopic impedance between intracellular and extracellular electrodes is significant, and cannot be captured by a model with resistive media. Fitting a mean-field model to the data shows that this frequency dependence could be explained by the ionic diffusion mainly associated with Debye layers surrounding the membranes. We conclude that neuronal membranes and their ionic environment induce strong deviations to resistivity that should be taken into account to correctly interpret extracellular potentials generated by neurons.


Subject(s)
Brain , Neurons , Electric Conductivity , Electric Impedance , Electrodes , Neurons/physiology
2.
Biophys J ; 113(7): 1639-1642, 2017 10 03.
Article in English | MEDLINE | ID: mdl-28978454

ABSTRACT

A recent commentary to Biophysical Journal criticized a previous study published in the same journal by Gomes et al. in 2016, and an alternative interpretation of the measurements was proposed. We reply here to these criticisms and provide some additional clarification, in particular, about a possible misinterpretation of the electrical circuit corresponding to these experiments. We suggest that, indeed, the extracellular impedance in cerebral cortex could be high and non-resistive, and we propose further experiments to settle this issue.


Subject(s)
Brain , Cerebral Cortex , Biophysics , Electric Impedance
3.
J Integr Neurosci ; 16(1): 3-18, 2017.
Article in English | MEDLINE | ID: mdl-28891497

ABSTRACT

In this viewpoint article, we discuss the electric properties of the medium around neurons, which are important to correctly interpret extracellular potentials or electric field effects in neural tissue. We focus on how these electric properties shape the frequency scaling of brain signals at different scales, such as intracellular recordings, the local field potential (LFP), the electroencephalogram (EEG) or the magnetoencephalogram (MEG). These signals display frequency-scaling properties which are not consistent with resistive media. The medium appears to exert a frequency filtering scaling as 1/f, which is the typical frequency scaling of ionic diffusion. Such a scaling was also found recently by impedance measurements in physiological conditions. Ionic diffusion appears to be the only possible explanation to reconcile these measurements and the frequency-scaling properties found in different brain signals. However, other measurements suggest that the extracellular medium is essentially resistive. To resolve this discrepancy, we show new evidence that metal-electrode measurements can be perturbed by shunt currents going through the surface of the brain. Such a shunt may explain the contradictory measurements, and together with ionic diffusion, provides a framework where all observations can be reconciled. Finally, we propose a method to perform measurements avoiding shunting effects, thus enabling to test the predictions of this framework.


Subject(s)
Brain/physiology , Extracellular Space/metabolism , Models, Neurological , Neurons/physiology , Signal Processing, Computer-Assisted , Animals , Electricity , Electroencephalography , Humans , Magnetoencephalography , Microelectrodes
4.
Biophys J ; 110(1): 234-46, 2016 Jan 05.
Article in English | MEDLINE | ID: mdl-26745426

ABSTRACT

Determining the electrical properties of the extracellular space around neurons is important for understanding the genesis of extracellular potentials, as well as for localizing neuronal activity from extracellular recordings. However, the exact nature of these extracellular properties is still uncertain. Here, we introduce a method to measure the impedance of the tissue, one that preserves the intact cell-medium interface using whole-cell patch-clamp recordings in vivo and in vitro. We find that neural tissue has marked non-ohmic and frequency-filtering properties, which are not consistent with a resistive (ohmic) medium, as often assumed. The amplitude and phase profiles of the measured impedance are consistent with the contribution of ionic diffusion. We also show that the impact of such frequency-filtering properties is possibly important on the genesis of local field potentials, as well as on the cable properties of neurons. These results show non-ohmic properties of the extracellular medium around neurons, and suggest that source estimation methods, as well as the cable properties of neurons, which all assume ohmic extracellular medium, may need to be reevaluated.


Subject(s)
Extracellular Space/metabolism , Intracellular Space/metabolism , Neurons/cytology , Animals , Brain/cytology , Electric Impedance , Mice , Models, Neurological , Rats
5.
Article in English | MEDLINE | ID: mdl-25375539

ABSTRACT

Neurons generate magnetic fields which can be recorded with macroscopic techniques such as magnetoencephalography. The theory that accounts for the genesis of neuronal magnetic fields involves dendritic cable structures in homogeneous resistive extracellular media. Here we generalize this model by considering dendritic cables in extracellular media with arbitrarily complex electric properties. This method is based on a multiscale mean-field theory where the neuron is considered in interaction with a "mean" extracellular medium (characterized by a specific impedance). We first show that, as expected, the generalized cable equation and the standard cable generate magnetic fields that mostly depend on the axial current in the cable, with a moderate contribution of extracellular currents. Less expected, we also show that the nature of the extracellular and intracellular media influence the axial current, and thus also influence neuronal magnetic fields. We illustrate these properties by numerical simulations and suggest experiments to test these findings.


Subject(s)
Magnetic Fields , Models, Neurological , Neurons/physiology , Computer Simulation , Dendrites/physiology , Electricity , Extracellular Space/physiology , Synaptic Transmission/physiology
6.
Article in English | MEDLINE | ID: mdl-24032866

ABSTRACT

Cable theory has been developed over the last decade, usually assuming that the extracellular space around membranes is a perfect resistor. However, extracellular media may display more complex electrical properties due to various phenomena, such as polarization, ionic diffusion, or capacitive effects, but their impact on cable properties is not known. In this paper, we generalize cable theory for membranes embedded in arbitrarily complex extracellular media. We outline the generalized cable equations, then consider specific cases. The simplest case is a resistive medium, in which case the equations recover the traditional cable equations. We show that for more complex media, for example, in the presence of ionic diffusion, the impact on cable properties such as voltage attenuation can be significant. We illustrate this numerically, always by comparing the generalized cable to the traditional cable. We conclude that the nature of intracellular and extracellular media may have a strong influence on cable filtering as well as on the passive integrative properties of neurons.


Subject(s)
Models, Biological , Neurons/cytology , Cytoplasm/metabolism , Dendrites/metabolism , Diffusion , Extracellular Space/metabolism , Membrane Potentials
7.
J Neurophysiol ; 109(6): 1683, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23503557
9.
J Neurosci Methods ; 210(1): 3-14, 2012 Sep 15.
Article in English | MEDLINE | ID: mdl-21968037

ABSTRACT

Variations of excitatory and inhibitory conductances determine the membrane potential (V(m)) activity of neurons, as well as their spike responses, and are thus of primary importance. Methods to estimate these conductances require clamping the cell at several different levels of V(m), thus making it impossible to estimate conductances from "single trial" V(m) recordings. We present here a new method that allows extracting estimates of the full time course of excitatory and inhibitory conductances from single-trial V(m) recordings. This method is based on oversampling of the V(m). We test the method numerically using models of increasing complexity. Finally, the method is evaluated using controlled conductance injection in cortical neurons in vitro using the dynamic-clamp technique. This conductance extraction method should be very useful for future in vivo applications.


Subject(s)
Action Potentials/physiology , Excitatory Postsynaptic Potentials/physiology , Inhibitory Postsynaptic Potentials/physiology , Models, Neurological , Neural Inhibition/physiology , Patch-Clamp Techniques/methods , Algorithms , Animals , Humans , Neurons/physiology
10.
Phys Rev E Stat Nonlin Soft Matter Phys ; 84(4 Pt 1): 041909, 2011 Oct.
Article in English | MEDLINE | ID: mdl-22181177

ABSTRACT

The current-source density (CSD) analysis is a widely used method in brain electrophysiology, but this method rests on a series of assumptions, namely that the surrounding extracellular medium is resistive and uniform, and in some versions of the theory, that the current sources are exclusively made by dipoles. Because of these assumptions, this standard model does not correctly describe the contributions of monopolar sources or of nonresistive aspects of the extracellular medium. We propose here a general framework to model electric fields and potentials resulting from current source densities, without relying on the above assumptions. We develop a mean-field formalism that is a generalization of the standard model and that can directly incorporate nonresistive (nonohmic) properties of the extracellular medium, such as ionic diffusion effects. This formalism recovers the classic results of the standard model such as the CSD analysis, but in addition, we provide expressions to generalize the CSD approach to situations with nonresistive media and arbitrarily complex multipolar configurations of current sources. We found that the power spectrum of the signal contains the signature of the nature of current sources and extracellular medium, which provides a direct way to estimate those properties from experimental data and, in particular, estimate the possible contribution of electric monopoles.


Subject(s)
Algorithms , Brain/physiology , Models, Neurological , Animals , Computer Simulation , Electric Impedance , Humans
11.
Philos Trans A Math Phys Eng Sci ; 369(1952): 3802-19, 2011 Oct 13.
Article in English | MEDLINE | ID: mdl-21893529

ABSTRACT

Rhythmic local field potential (LFP) oscillations observed during deep sleep are the result of synchronized electrical activities of large neuronal ensembles, which consist of alternating periods of activity and silence, termed 'up' and 'down' states, respectively. Current-source density (CSD) analysis indicates that the up states of these slow oscillations are associated with current sources in superficial cortical layers and sinks in deep layers, while the down states display the opposite pattern of source-sink distribution. We show here that a network model of up and down states displays this CSD profile only if a frequency-filtering extracellular medium is assumed. When frequency filtering was modelled as inhomogeneous conductivity, this simple model had considerably more power in slow frequencies, resulting in significant differences in LFP and CSD profiles compared with the constant-resistivity model. These results suggest that the frequency-filtering properties of extracellular media may have important consequences for the interpretation of the results of CSD analysis.


Subject(s)
Brain Waves/physiology , Electric Conductivity , Extracellular Space/metabolism , Models, Neurological , Cerebral Cortex/cytology , Cerebral Cortex/physiology , Kinetics , Neurons/cytology , Sleep/physiology , Thalamus/cytology , Thalamus/physiology
12.
J Comput Neurosci ; 29(3): 405-21, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20697790

ABSTRACT

The resistive or non-resistive nature of the extracellular space in the brain is still debated, and is an important issue for correctly modeling extracellular potentials. Here, we first show theoretically that if the medium is resistive, the frequency scaling should be the same for electroencephalogram (EEG) and magnetoencephalogram (MEG) signals at low frequencies (<10 Hz). To test this prediction, we analyzed the spectrum of simultaneous EEG and MEG measurements in four human subjects. The frequency scaling of EEG displays coherent variations across the brain, in general between 1/f and 1/f(2), and tends to be smaller in parietal/temporal regions. In a given region, although the variability of the frequency scaling exponent was higher for MEG compared to EEG, both signals consistently scale with a different exponent. In some cases, the scaling was similar, but only when the signal-to-noise ratio of the MEG was low. Several methods of noise correction for environmental and instrumental noise were tested, and they all increased the difference between EEG and MEG scaling. In conclusion, there is a significant difference in frequency scaling between EEG and MEG, which can be explained if the extracellular medium (including other layers such as dura matter and skull) is globally non-resistive.


Subject(s)
Brain/physiology , Electroencephalography/statistics & numerical data , Extracellular Space/physiology , Magnetoencephalography/statistics & numerical data , Adult , Algorithms , Data Interpretation, Statistical , Dendrites/physiology , Electrocardiography , Electromagnetic Fields , Fourier Analysis , Humans , Male , Signal Processing, Computer-Assisted , Skull/physiology , Young Adult
13.
J Comput Neurosci ; 29(3): 389-403, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20559865

ABSTRACT

We examine the properties of the transfer function F(T)=V(m)/V(LFP) between the intracellular membrane potential (V(m)) and the local field potential (V(LFP)) in cerebral cortex. We first show theoretically that, in the subthreshold regime, the frequency dependence of the extracellular medium and that of the membrane potential have a clear incidence on F(T). The calculation of F(T) from experiments and the matching with theoretical expressions is possible for desynchronized states where individual current sources can be considered as independent. Using a mean-field approximation, we obtain a method to estimate the impedance of the extracellular medium without injecting currents. We examine the transfer function for bipolar (differential) LFPs and compare to simultaneous recordings of V(m) and V(LFP) during desynchronized states in rat barrel cortex in vivo. The experimentally derived F(T) matches the one derived theoretically, only if one assumes that the impedance of the extracellular medium is frequency-dependent, and varies as 1/√ω (Warburg impedance) for frequencies between 3 and 500 Hz. This constitutes indirect evidence that the extracellular medium is non-resistive, which has many possible consequences for modeling LFPs.


Subject(s)
Electric Impedance , Evoked Potentials/physiology , Extracellular Space/physiology , Intracellular Space/physiology , Action Potentials/physiology , Algorithms , Animals , Cell Membrane/physiology , Computer Simulation , Electroencephalography , Electroencephalography Phase Synchronization , Electrophysiological Phenomena , Linear Models , Male , Membrane Potentials/physiology , Models, Neurological , Rats , Rats, Sprague-Dawley , Somatosensory Cortex/physiology
14.
J Comput Neurosci ; 2010 Jun 17.
Article in English | MEDLINE | ID: mdl-20556640

ABSTRACT

The resistive or non-resistive nature of the extracellular space in the brain is still debated, and is an important issue for correctly modeling extracellular potentials. Here, we first show theoretically that if the medium is resistive, the frequency scaling should be the same for electroencephalogram (EEG) and magnetoencephalogram (MEG) signals at low frequencies (<10 Hz). To test this prediction, we analyzed the spectrum of simultaneous EEG and MEG measurements in four human subjects. The frequency scaling of EEG displays coherent variations across the brain, in general between 1/f and 1/f (2). In a given region, although the variability of the frequency scaling exponent was higher for MEG compared to EEG, both signals consistently scale with a different exponent. In some cases, the scaling was similar, but only when the signal-to-noise ratio of the MEG was low. Several methods of noise correction for environmental and instrumental noise were tested, and they all increased the difference between EEG and MEG scaling. In conclusion, there is a significant difference in frequency scaling between EEG and MEG, which can be explained if the extracellular medium (including other layers such as dura matter and skull) is globally non-resistive.

15.
Biophys J ; 96(7): 2589-603, 2009 Apr 08.
Article in English | MEDLINE | ID: mdl-19348744

ABSTRACT

The power spectrum of local field potentials (LFPs) has been reported to scale as the inverse of the frequency, but the origin of this 1/f noise is at present unclear. Macroscopic measurements in cortical tissue demonstrated that electric conductivity (as well as permittivity) is frequency-dependent, while other measurements failed to evidence any dependence on frequency. In this article, we propose a model of the genesis of LFPs that accounts for the above data and contradictions. Starting from first principles (Maxwell equations), we introduce a macroscopic formalism in which macroscopic measurements are naturally incorporated, and also examine different physical causes for the frequency dependence. We suggest that ionic diffusion primes over electric field effects, and is responsible for the frequency dependence. This explains the contradictory observations, and also reproduces the 1/f power spectral structure of LFPs, as well as more complex frequency scaling. Finally, we suggest a measurement method to reveal the frequency dependence of current propagation in biological tissue, and which could be used to directly test the predictions of this formalism.


Subject(s)
Brain/physiology , Models, Biological , Brain/cytology , Brain/metabolism , Diffusion , Electric Conductivity , Extracellular Space/metabolism , Microscopy
16.
Biophys J ; 94(4): 1133-43, 2008 Feb 15.
Article in English | MEDLINE | ID: mdl-17921220

ABSTRACT

Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditional models, as either single-compartment models or models based on reconstructed cell morphologies display a frequency scaling with a slope close to -4. This slope is due to the fact that the membrane resistance is short-circuited by the capacitance for high frequencies, a situation which may not be realistic. Here, we integrate nonideal capacitors in cable equations to reflect the fact that the capacitance cannot be charged instantaneously. We show that the resulting nonideal cable model can be solved analytically using Fourier transforms. Numerical simulations using a ball-and-stick model yield membrane potential activity with similar frequency scaling as in the experiments. We also discuss the consequences of using nonideal capacitors on other cellular properties such as the transmission of high frequencies, which is boosted in nonideal cables, or voltage attenuation in dendrites. These results suggest that cable equations based on nonideal capacitors should be used to capture the behavior of neuronal membranes at high frequencies.


Subject(s)
Action Potentials/physiology , Cell Membrane/physiology , Membrane Potentials/physiology , Models, Neurological , Neurons/physiology , Synaptic Transmission/physiology , Animals , Computer Simulation , Humans
17.
Biophys J ; 86(3): 1829-42, 2004 Mar.
Article in English | MEDLINE | ID: mdl-14990509

ABSTRACT

Extracellular local field potentials are usually modeled as arising from a set of current sources embedded in a homogeneous extracellular medium. Although this formalism can successfully model several properties of extracellular local field potentials, it does not account for their frequency-dependent attenuation with distance, a property essential to correctly model extracellular spikes. Here we derive expressions for the extracellular potential that include this frequency-dependent attenuation. We first show that, if the extracellular conductivity is nonhomogeneous, there is induction of nonhomogeneous charge densities that may result in a low-pass filter. We next derive a simplified model consisting of a punctual (or spherical) current source with spherically symmetric conductivity/permittivity gradients around the source. We analyze the effect of different radial profiles of conductivity and permittivity on the frequency-filtering behavior of this model. We show that this simple model generally displays low-pass filtering behavior, in which fast electrical events (such as Na(+)-mediated action potentials) attenuate very steeply with distance, whereas slower (K(+)-mediated) events propagate over larger distances in extracellular space, in qualitative agreement with experimental observations. This simple model can be used to obtain frequency-dependent extracellular field potentials without taking into account explicitly the complex folding of extracellular space.


Subject(s)
Action Potentials/physiology , Electromagnetic Fields , Extracellular Space/physiology , Membrane Potentials/physiology , Models, Neurological , Neurons/physiology , Synaptic Transmission/physiology , Animals , Computer Simulation , Humans
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