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1.
Sci Rep ; 12(1): 4171, 2022 03 09.
Article in English | MEDLINE | ID: mdl-35264620

ABSTRACT

There is interest in utilizing wood ash as an amendment in forestry operations as a mechanism to return nutrients to soils that are removed during harvesting, with the added benefit of diverting this bioenergy waste material from landfill sites. Existing studies have not arrived at a consensus on what the effects of wood ash amendments are on soil biota. We collected forest soil samples from studies in managed forests across Canada that were amended with wood ash to evaluate the effects on arthropod, bacterial and fungal communities using metabarcoding of F230, 16S, 18S and ITS2 sequences as well as enzyme analyses to assess its effects on soil biotic function. Ash amendment did not result in consistent effects across sites, and those effects that were detected were small. Overall, this study suggests that ash amendment applied to managed forest systems in amounts (up to 20 Mg ha-1) applied across the 8 study sties had little to no detectable effects on soil biotic community structure or function. When effects were detected, they were small, and site-specific. These non-results support the application of wood ash to harvested forest sites to replace macronutrients (e.g., calcium) removed by logging operations, thereby diverting it from landfill sites, and potentially increasing stand productivity.


Subject(s)
Soil Pollutants , Soil , Biota , Forestry , Forests , Soil/chemistry , Soil Pollutants/analysis
2.
Can J Microbiol ; 59(6): 399-406, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23750954

ABSTRACT

Naturally occurring nitrogen-fixing symbionts from root nodules of caragana (Caragana arborescens) growing in central Saskatchewan were isolated following surface sterilization of caragana root nodules and squashing and spreading of the contents on yeast extract - mannitol medium. The symbiotic nature of the strains was confirmed following inoculation onto surface-sterilized C. arborescens seed in a gnotobiotic Leonard jar system. The Rhizobium isolates from C. arborescens root nodules were intermediate in generation time (g) (mean g of 5 isolates was 6.41 h) compared with the fast growers, Rhizobium leguminosarum NRG457 (g: 4.44 h), Rhizobium tropici 899 (g: 3.19 h), and Sinorhizobium meliloti BALSAC (g: 3.45 h), but they were faster than the slow-growing Bradyrhizobium japonicum USDA 110 (g: 13.86 h) and similar to Mesorhizobium amorphae (g: 7.76 h). Nitrogen derived from fixation by measuring changes in δ(15)N natural abundance in plant tissue confirmed the effectiveness of the strains; approximately 80% N2 from fixation. Strain identification was carried out by determining the sequences of 3 genes: 16S rRNA-encoding genes, cpn60, and recA. This analysis determined that the symbiotic partner of Canadian C. arborescens belongs to the genus Mesorhizobium and seems more related to M. loti than to previously described caragana symbionts like M. caraganae. This is the first report of Mesorhizobium sp. nodulating C. arborescens in western Canada.


Subject(s)
Caragana/microbiology , Mesorhizobium/classification , Mesorhizobium/physiology , Root Nodules, Plant/microbiology , Symbiosis , Carbon/analysis , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Genes, Bacterial , Genes, rRNA , Mesorhizobium/isolation & purification , Mesorhizobium/metabolism , Nitrogen/analysis , Nitrogen Fixation , Phylogeny , Plant Leaves/chemistry , RNA, Ribosomal, 16S/genetics , Saskatchewan
3.
J Neurophysiol ; 105(6): 3022-33, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21451055

ABSTRACT

There is considerable debate on the extent to which biomechanical properties of movements are taken into account before and during voluntary movements. For example, while several models have described reach planning as primarily kinematic, some studies have suggested that implicit knowledge about biomechanics may also exert some influence on the planning of reaching movements. Here, we investigated whether decisions about reaching movements are influenced by biomechanical factors and whether these factors are taken into account before movement onset. To this end, we designed an experimental paradigm in which humans made free choices between two potential reaching movements where the options varied in path distance as well as biomechanical factors related to movement energy and stability. Our results suggest that the biomechanical properties of potential actions strongly influence the selection between them. In particular, in our task, subjects preferred movements whose final trajectory was better aligned with the major axis of the arm's mobility ellipse, even when the launching properties were very similar. This reveals that the nervous system can predict biomechanical properties of potential actions before movement onset and that these predictions, in addition to purely abstract criteria, may influence the decision-making process.


Subject(s)
Arm/physiology , Biomechanical Phenomena/physiology , Decision Making , Movement/physiology , Adult , Electromyography , Evoked Potentials, Motor/physiology , Female , Humans , Male , Models, Biological , Muscle, Skeletal/physiology , Predictive Value of Tests , Torque
4.
Opt Express ; 13(8): 3068-78, 2005 Apr 18.
Article in English | MEDLINE | ID: mdl-19495203

ABSTRACT

The use of a complex short-period (Bragg) grating which combines matched periodic modulations of refractive index and loss/gain allows asymmetrical mode coupling within a contra-directional waveguide coupler. Such a complex Bragg grating exhibits a different behavior (e.g. in terms of the reflection and transmission spectra) when probed from opposite ends. More specifically, the grating has a single reflection peak when used from one end, but it is transparent (zero reflection) when used from the opposite end. In this paper, we conduct a systematic analytical and numerical analysis of this new class of Bragg gratings. The spectral performance of these, so-called nonreciprocal gratings, is first investigated in detail and the influence of device parameters on the transmission spectra of these devices is also analyzed. Our studies reveal that in addition to the nonreciprocal behavior, a nonreciprocal Bragg grating exhibits a strong amplification at the resonance wavelength (even with zero net-gain level in the waveguide) while simultaneously providing higher wavelength selectivity than the equivalent index Bragg grating. However, it is also shown that in order to achieve non-reciprocity in the device, a very careful adjustment of the parameters corresponding to the index and gain/loss gratings is required.

5.
Opt Express ; 13(9): 3567-78, 2005 May 02.
Article in English | MEDLINE | ID: mdl-19495261

ABSTRACT

A recently proposed concept suggests that a matched periodic modulation of both the refractive index and the gain/loss of the media breaks the coupling symmetry of the two co-propagating modes and allows only a unidirectional coupling from the i-th mode to j-the mode but not the opposite. This concept has been used to design a ring resonator coupled through a complex grating composed of both real (index) and imaginary (loss/gain) parts according to Euler relation: n = n0 exp(-jkx) = n0 (cos(kx) - j sin(kx)). Such asymmetrical coupling allows light to be coupled into the ring without letting it out. We present a detailed theoretical analysis of the ring resonator in the linear regime, and we investigate its linear temporal dynamics. Three possible states of the complex grating leads to the possibility of developing a dynamic optical memory cell where, for example, a data modulated train of optical pulses can be stored. This data can be accessed without destroying it, and can also be erased thus permitting the storage of a new bit. Finally, the ring can be used for pulse retiming.

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