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1.
Zygote ; 22(4): 558-64, 2014 Nov.
Article in English | MEDLINE | ID: mdl-24152610

ABSTRACT

The aim of the study was to determine the viability of rabbit transgenic (enhanced green fluorescent protein (EGFP)-positive) embryos cultured in vitro and compare with gene-microinjected (Mi) non-transgenic (EGFP-negative) embryos following vitrification. Non-microinjected and non-vitrified embryos were used as the control. Morphological signs of injury to embryo organelles were determined at the ultrastructural level using transmission electron microscopy (TEM). Morphometric evaluation was performed on cellular organelles using microphotographs obtained by TEM. Intact and Mi embryos recovered from in vivo fertilized eggs at 19-20 hours post coitum (hpc) were cultured for up to 72 hpc (morula stage), evaluated for the EGFP gene integration and then vitrified in 0.25 ml insemination straws in modified EFS (40% ethylene glycol + 18% Ficoll 70 + 0.3 M sucrose) vitrification solution. After 1-3 days the embryos were devitrified, a representative selection of embryos was analyzed by TEM and the remaining embryos were subjected to additional in vitro culture. Observations by TEM showed that the vitrified/warmed EGFP-positive and EGFP-negative embryos had a slight accumulation of cellular debris and lipid droplets compared with the control intact embryos. More severe changes were detected in the membrane structures of the treated embryos, mostly in the cytoplasmic envelope, trophoblastic microvilli, junctional contacts and mitochondria. We suggest that the higher proportion of deteriorated cell structures and organelles in the treated embryos may be due to the vitrification process rather than to mechanical violation (the gene-microinjection procedure), as a detailed inspection of ultrastructure revealed that most damage occurred in the cell membrane structures.


Subject(s)
Embryo, Mammalian/ultrastructure , Vitrification , Animals , Animals, Genetically Modified , Female , Green Fluorescent Proteins/genetics , Male , Microinjections , Microscopy, Electron, Transmission , Morula , Rabbits
2.
Transgenic Res ; 19(5): 799-808, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20069454

ABSTRACT

Transgenic rabbit is the preferred disease model of atherosclerosis, lipoprotein metabolism and cardiovascular diseases since upon introducing genetic mutations of human genes, rabbit models reflect human physiological and pathological states more accurately than mouse models. Beyond that, transgenic rabbits are also used as bioreactors to produce pharmaceutical proteins in their milk. Since in the laboratory rabbit the conventional transgenesis has worked with the same low efficiency in the last twenty five years and truly pluripotent embryonic stem cells are not available to perform targeted mutagenesis, our aim was to adapt lentiviral transgenesis to this species. A simian immunodeficiency virus based replication defective lentiviral vector was used to create transgenic rabbit through perivitelline space injection of fertilized oocytes. The enhanced green fluorescent protein (GFP) gene was placed under the ubiquitous CAG promoter. Transgenic founder rabbits showed mosaic pattern of GFP expression. Transgene integration and expression was revealed in tissues derived from all three primary germ layers. Transgene expression was detected in the developing sperm cells and could get through the germ line without epigenetic silencing, albeit with very low frequency. Our data show for the first time, that lentiviral transgenesis could be a feasible and viable alternative method to create genetically modified laboratory rabbit.


Subject(s)
Animals, Genetically Modified/genetics , Genetic Vectors/genetics , Rabbits/genetics , Simian Immunodeficiency Virus/genetics , Transgenes , Animals , Embryo Transfer , Feasibility Studies , Female , Gene Expression Regulation , Gene Silencing , Genes, Reporter , Green Fluorescent Proteins/genetics , Male , Microinjections , Mosaicism , Organ Specificity , Spermatozoa/chemistry , Zygote
3.
Transgenic Res ; 15(5): 627-36, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16826424

ABSTRACT

Alkaline phosphatase is a promising therapeutic agent in the Gram-negative bacterial lipopolysaccharide (LPS) mediated acute and chronic diseases. Contrary to other alkaline phosphatase isozymes, purified tissue-nonspecific alkaline phosphatase (TNAP) is not available in large quantities from tissue sources, which would enable to analyse its efficacy in animal sepsis models. Two transgenic rabbit lines were created by pronuclear microinjection with the whey acidic protein promoter-humanTNAP minigene (WAP-hTNAP). Lactating females of both lines produced biologically active human TNAP. As indicated by fractionation of milk samples the recombinant alkaline phosphatase was associated with the membrane of milk fat globules. Alkaline phosphatase enzymatic activity was two orders of magnitude higher compared to normal human serum levels. The demonstration that this TNAP is physiologically active would provide the clue to use transgenic animals as bioreactor for bulk production of the human tissue-nonspecific alkaline phosphatase in milk. This may be a valuable and possibly viable option with important implication in attenuating LPS mediated inflammatory responses.


Subject(s)
Alkaline Phosphatase/biosynthesis , Alkaline Phosphatase/genetics , Animals, Genetically Modified , Milk/enzymology , Rabbits/genetics , Animals , Female , Humans , Mice
4.
Transgenic Res ; 12(5): 541-53, 2003 Oct.
Article in English | MEDLINE | ID: mdl-14601653

ABSTRACT

Until recently, transgenic rabbits were produced exclusively by pronuclear microinjection which results in additive random insertional transgenesis; however, progress in somatic cell cloning based on nuclear transfer will soon make it possible to produce rabbits with modifications to specific genes by the combination of homologous recombination and subsequent prescreening of nuclear donor cells. Transgenic rabbits have been found to be excellent animal models for inherited and acquired human diseases including hypertrophic cardiomyopathy, perturbed lipoprotein metabolism and atherosclerosis. Transgenic rabbits have also proved to be suitable bioreactors for the production of recombinant protein both on an experimental and a commercial scale. This review summarizes recent research based on the transgenic rabbit model.


Subject(s)
Animals, Genetically Modified , Disease Models, Animal , Rabbits/genetics , Animals , Female , Gene Transfer Techniques , Hormones/genetics , Hormones/metabolism , Mammary Glands, Animal/metabolism , Peptides/genetics , Peptides/metabolism
5.
DNA Cell Biol ; 22(1): 41-5, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12590736

ABSTRACT

Human clotting factor VIII is probably the largest protein to be expressed to date in the mammary gland of a transgenic animal, and it requires extensive posttranslational modification to achieve full biological activity. The mammary gland specific construct mWAP-hFVIII-MT-I was injected into the pronuclei of rabbit zygotes, and three transgenic offspring were obtained. Founder 385 showed germ-line transmission of a single integrated copy, and a homozygous line was established from this animal. The rhFVIII was transcribed and translated exclusively in the mammary gland. The activity of rhFVIII in the rabbit milk ranged from 5 to 8% of that found in normal human plasma. Results indicate the suitability of the transgenic rabbit mammary gland for rhFVIII production.


Subject(s)
Animals, Genetically Modified/metabolism , Factor VIII/genetics , Mammary Glands, Animal/metabolism , Milk/metabolism , Animals , Blood Coagulation/physiology , Factor VIII/metabolism , Female , Humans , Lactation , Rabbits
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