rs-fMRI and machine learning for ASD diagnosis: a systematic review and meta-analysis.

Autism Spectrum Disorder (ASD) diagnosis is still based on behavioral criteria through a lengthy and time-consuming process. Much effort is being made to identify brain imaging biomarkers and develop tools that could facilitate its diagnosis. In particular, using Machine Learning classifiers based on resting-state fMRI (rs-fMRI) data is promising, but there is an ongoing need for further research on their accuracy and reliability. Therefore, we conducted a systematic review and meta-analysis to summarize the available evidence in the literature so far. A bivariate random-effects meta-analytic model was implemented to investigate the sensitivity and specificity across the 55 studies that offered sufficient information for quantitative analysis. Our results indicated overall summary sensitivity and specificity estimates of 73.8% and 74.8%, respectively. SVM stood out as the most used classifier, presenting summary estimates above 76%. Studies with bigger samples tended to obtain worse accuracies, except in the subgroup analysis for ANN classifiers. The use of other brain imaging or phenotypic data to complement rs-fMRI information seems promising, achieving higher sensitivities when compared to rs-fMRI data alone (84.7% versus 72.8%). Finally, our analysis showed AUC values between acceptable and excellent. Still, given the many limitations indicated in our study, further well-designed studies are warranted to extend the potential use of those classification algorithms to clinical settings.

Synthesis, activity, and docking studies of eugenol-based glucosides as new agents against Candida sp.

Seventeen new synthetic derivatives of eugenol (6, 8-15 and 8'-15') were planned following literature reports on antifungal activities of nitroeugenol and eugenol glucoside. The anti-Candida activity of these compounds was investigated by in vitro assay, and the cytotoxicity evaluation was performed with the most active compounds. The peracetylated glucosides presented better biological results than their hydroxylated analogues. The glucoside 11, a 4-nitrobenzamide, showed the best potency (MIC50 range 11.0-151.84 µm), the wider spectrum of action, and overall the best selectivity indexes, especially against C. tropicalis (~30) and C. krusei (~15). To investigate its possible mechanism of action, glucoside 11 was subjected to molecular docking studies with Candida sp. enzymes involved in ergosterol biosynthesis. Results have shown that the peracetyl glucosyl moiety and the 4-nitrobenzamide group in 11 are effectively involved in its high affinity with the active site of squalene epoxidase.

Phenotypic and genotypic characteristics on calves' sale price on the western border of the state of Rio Grande do Sul, Brazil / Impacto de características fenotípicas e genotípicas no preço de venda de bezerros na fronteira oeste do Rio Grande do Sul, Brasil

ABSTRACT: The demand for beef cattle with specific traits is evident from sales in auctions. While purchasing, buyers consider individual traits (e.g., muscularity, frame, racial composition, and weight) as predictors of quality, and adjust their bids according to their preferences. The present study aimed to evaluate the effect of phenotypic and racial characteristics in determining the final sale price of calves of beef cattle in official auctions at the western border of the state of Rio Grande do Sul, Brazil. Five-hundred and seven lots of calves sold in calf auctions from five cities on the western border of Rio Grande do Sul were evaluated. The assignment of visual scores was based on their genetic group, frame, muscularity, and coat. Analysis of variance and the Tukey test at 5% significance level were performed for comparing the final average prices. Variables with the greatest impact on price were the genetic group and the size of the animals. Animals of synthetic breeds obtained higher value in the three years studied and animals without racial definition showed considerable devaluation and a decrease in supply. Larger animals had less value.

RESUMO: A busca por animais com características específicas é evidenciada na comercialização em leilões, em que, ao analisar os animais, compradores consideram as características individuais (musculosidade, frame, composição racial, peso) como preditor de qualidade e ajustam seus lances conforme suas preferências. Dessa forma, o presente estudo buscou avaliar o impacto de características fenotípicas e de padrão racial na composição do preço final de venda de bezerros comercializados em feiras oficiais na fronteira oeste do Rio Grande do Sul. Foram avaliados 507 lotes de bezerros comercializados nas feiras de terneiros de cinco municípios da região da Fronteira Oeste do estado do Rio Grande do Sul. Para a atribuição de escores visuais, considerou-se grupo genético, frame, musculosidade e pelagem dos animais. Foi realizada a análise de variância e a comparação dos preços finais médios pelo teste de Tukey a 5% de significância. As variáveis de maior impacto no preço foram grupo genético e tamanho dos animais. Animais de raças sintéticas obtiveram valorização superior nos três anos Estudados, considerável desvalorização e queda na oferta de animais sem definição racial. Os menores valores foram atribuídos a animais de tamanho grande.

Immunolocalization of the high-mobility group N2 protein and acetylated histone H3K14 in early developing parthenogenetic bovine embryos derived from oocytes of high and low developmental competence.

This study investigated differences in the distribution of acetylated histone H3 at Lysine 14 (H3K14ac) and the High-Mobility Group N2 (HMGN2) protein in the chromatin of early- (before 24 hr) and late-cleaved (after 24 hr) bovine embryos derived from small- (1-2 mm) and large-follicles (4-8 mm). The presence of HMGN2 and H3K14ac has been associated with different nuclear functions including chromatin condensation, transcription, DNA replication and repair. In vitro matured oocytes were parthenogenetically activated (PA) and cultured in synthetic oviduct fluid medium. Early- and late-cleaved embryos were fixed at 36, 50, 60, 70 and 80 hr after PA to detect the presence of H3K14ac and HMGN2. The rates of nuclear maturation (81.1% vs. 58.7%), early cleavage (46.9% vs. 38.9%), and development to blastocyst stage (34.3% vs. 18.9%) were higher (P < 0.05) in oocytes derived from large- compared to small follicles. The proportion of positively stained nuclei at 50 and 60 hr after PA was higher for both H3K14ac (27.2% vs. 4.8% and 64.3% vs. 30%) and HMGN2 (47% vs. 21.3% and 60.6% vs. 46%) in early versus late cleaved embryos derived from small- versus large-follicles, respectively. However, the rate of positive nuclei in early-cleaved embryos from small-versus large-follicles was similar for HMGN2 (87% vs. 93%) but lower for H3K14ac (51% vs. 64.4%) at 80 hr after PA. These data suggest that less developmentally competent embryos derived from small follicles had an altered chromatin remodeling process at the early stages of development compared to those derived from large follicles that are more competent to support development to blastocyst stage.

Optimal load distribution between units in a power plant.

This paper presents a strategy for load distribution between the generating units in hydro power plants. The objective is to reach the maximum energy conversion efficiency for a given dispatched power. The developed tool employs a heuristic-based combinatorial optimization technique in conjunction with a set of system variables measurement allowing real-time load sharing. The developed equipment is used to give online energy conversion efficiency from each unit of the power plant. No specific previous information about the efficiency of system components is required. Simulation results of the proposed optimization technique when applied to typical hydro power plant data are presented.

Hormonal induction of ovulation and artificial insemination in suckled beef cows under nutritional stress.

The objective was to develop a program for inducing estrus (followed by insemination) of suckled beef cows under nutritional stress (poor body condition). A total of 123 cows, from 60 to 75 days postpartum, were classified according to their body condition score (BCS; range from 1 to 5, in increments of 0.5) and allocated into two groups. On Day 0 (without regard to stage of the estrous cycle), cows (n = 59) in the hormone induction (HI) treatment group were given an intravaginal device (IVD) containing 250 mg of medroxiprogesterone acetate (MAP) and an i.m. injection of 2.5 mg estradiol benzoate (EB). On Day 6, these cows were given 500 IU eCG i.m. and calves were weaned for 96 h. The IVD were removed on Day 7. Cows detected in estrus by 45 h after IVD removal were inseminated 12 h after standing estrus; cows not in estrus by 45 h after IVD removal received an i.m. injection of 100 microg gonadorelin (GnRH) and were inseminated 16-18 h later. In the control group (C), cows (n = 64) only had their calves weaned at Day 6 (for 96 h), with estrus detection and AI from Days 6 to 11. Overall, the BCS ranged from 2.0 to 3.0. In the treatment group, estrus and pregnancy rates in cows with BCS 2.0 (20 and 30%, respectively) was lower (P < 0.05) than those with BCS 3.0 (50 and 66.6%, respectively), but did not differ (P > 0.05) from BCS 2.5 (23.3 and 47.6%). In C group, only 2 of 66 cows were detected in estrus and bred (neither was pregnant). In conclusion, the program for induction of ovulation using MAP, EB, eCG and GnRH increased the pregnancy rate in beef cows in poor body condition, enabling AI to be done in a 63-h interval.

Ejaculacao precoce: proposta baseada na cronologia

Em um trabalho realizado anteriormente (11), constatou-se um alto indice de evasao dos pacientes com ejaculacao precoce, no processo de psicoterapia ou terapia convencional. Na observacao dos autores isto ocorreu principalemte nos pacientes com queixa de cronologia superior a 4 anos e que se mostraram desmotivados as terapias citadas. No presente trabalho indicou-se para um grupo de 26 pacientes, durante 1992, na faixa etaria de 22 a 60 anos, desde o inicio da terapia, a clomipramina. Tal indicacao objetivou a acao da medicacao na ejaculacao, bem como seu efeito complementar sobre a ansiedade e possiveis quadros depressivos reativos mascarados que pudessem acompanhar esses pacientes. A interacao foi de possibilitar uma melhor adequacao sexual, facilitando o processo psicoterapeutico. Observou-se neste trabalho, uma diminuicao do indice de evasao em relacao aos pacientes que nao usaram a clomipramina, estudados no trabalho anterior (11). Desta forma, os autores concluem que a clomipramina associada a terapia beneficia aos pacientes com ejaculacao precoce, visto que, proporcionando um melhor controle jaculatorio, com diminuicao da ansiedade, estimula o paciente ao processo psicoterapeutico.

Prenhez ovariana íntegra / Total ovarian pregnancy

Os autores relatam um caso de prenhez ovariana íntegra confirmado ao exame histopatológico e de escasso registro na literatura médica devido à raridade do caso

Prenhez tubária perfurada em gestaçäo avançada / Late perforated tubal pregnancy

Apresenta-se um caso de prenhez tubária perfurada em gestaçäo avançada na 21ª semana com morte fetal recente

Focomelia / Focomelia

Apresenta-se um caso de malformaçäo congênita - focomelia - em que näo há uma etiologia definida devido ao acompanhamento pré-natal, com o desfecho de um parto prematuro de um feto neomorto

Abortamento precoce evoluindo para eliminaçäo ou reabsorçäo sob controle ultra-sônico / Early abortion developing for elimination a reabsorption under ultrasonic control

Os autores procuram mostrar que, com o advento da ultra-sonografia, o abortamento precoce pode evoluir para reabsorçäo ou eliminaçäo, sem necessidade de curetagem uterina, principalmente naqueles casos de ovo anembrionado em que, outrora, somente diante da clínica e de posse do teste imunológico para gravidez, curetava-se a paciente com o diagnóstico de abortamento retido