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1.
Opt Lett ; 43(9): 2058-2061, 2018 May 01.
Article in English | MEDLINE | ID: mdl-29714745

ABSTRACT

Hyperspectral imaging in scattering tissue generally suffers from low light collection efficiency. In this Letter, we propose a microscope based on Fourier transform spectroscopy and oblique back-illumination microscopy that provides hyperspectral phase and amplitude images of thick, scattering samples with high throughput. Images can be acquired at >0.1 Hz rates with spectral resolution better than 200 cm-1, over a wide spectral range of 450-1700 nm. Proof-of-principle demonstrations are presented with chorioallantoic membrane of a chick embryo, illustrating the possibility of high-resolution hemodynamics imaging in thick tissue, based on transmission contrast.


Subject(s)
Chorioallantoic Membrane/blood supply , Diagnostic Imaging , Microcirculation/physiology , Microscopy/methods , Animals , Chick Embryo , Fourier Analysis , Phantoms, Imaging
2.
Biomed Opt Express ; 9(12): 6145-6153, 2018 Dec 01.
Article in English | MEDLINE | ID: mdl-31065419

ABSTRACT

We present a fast label-free computational flow cytometer based on a strategy of compressive imaging. Scattered light from flowing objects is sub-divided into user-defined basis patterns by a deformable mirror and routed to different detectors associated with each pattern. The patterns can be optimized to be matched to the object features of interest, thus facilitating object identification and separation. Compared to conventional scanning flow cytometers, our technique provides increased information capacity without sacrificing flow velocity. Unique features of our matched-filter strategy are that it can simultaneously probe multiple objects throughout large fields of view with long depths of field. In our proof-of-concept demonstrations, we achieve throughputs of over 10,000 particles/s, working at flow velocities of over 1m/s.

3.
BMC Syst Biol ; 11(1): 1, 2017 01 06.
Article in English | MEDLINE | ID: mdl-28061857

ABSTRACT

BACKGROUND: Enteric Escherichia coli survives the highly acidic environment of the stomach through multiple acid resistance (AR) mechanisms. The most effective system, AR2, decarboxylates externally-derived glutamate to remove cytoplasmic protons and excrete GABA. The first described system, AR1, does not require an external amino acid. Its mechanism has not been determined. The regulation of the multiple AR systems and their coordination with broader cellular metabolism has not been fully explored. RESULTS: We utilized a combination of ChIP-Seq and gene expression analysis to experimentally map the regulatory interactions of four TFs: nac, ntrC, ompR, and csiR. Our data identified all previously in vivo confirmed direct interactions and revealed several others previously inferred from gene expression data. Our data demonstrate that nac and csiR directly modulate AR, and leads to a regulatory network model in which all four TFs participate in coordinating acid resistance, glutamate metabolism, and nitrogen metabolism. This model predicts a novel mechanism for AR1 by which the decarboxylation enzymes of AR2 are used with internally derived glutamate. This hypothesis makes several testable predictions that we confirmed experimentally. CONCLUSIONS: Our data suggest that the regulatory network underlying AR is complex and deeply interconnected with the regulation of GABA and glutamate metabolism, nitrogen metabolism. These connections underlie and experimentally validated model of AR1 in which the decarboxylation enzymes of AR2 are used with internally derived glutamate.


Subject(s)
Escherichia coli/physiology , Protein Interaction Mapping , Computational Biology , Escherichia coli/drug effects , Escherichia coli/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Gene Expression Profiling , Hydrogen-Ion Concentration , Phenotype
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