ABSTRACT
A bienzymatic supramolecular assembly of CAT and SOD is reported. CAT was chemically glycosilated with CD branched CMC and then associated with SOD modified with 1-adamantane carboxylic acid. SOD was remarkably resistant to inactivation by H(2)O(2) and its anti-inflammatory activity was 4.5-fold increased after supramolecular association with the modified CAT form. [structure: see text]
Subject(s)
Anti-Inflammatory Agents/chemistry , Catalase/chemistry , Superoxide Dismutase/chemistry , Animals , Carbohydrate Conformation , Carrageenan , Catalase/blood , Cattle , Edema/chemically induced , Edema/drug therapy , Erythrocytes/enzymology , Glycosylation , Hydrogen Peroxide/metabolism , Kinetics , Macromolecular Substances , Male , Models, Molecular , Rats , Rats, Wistar , Superoxide Dismutase/bloodABSTRACT
Mannan and carboxymethylcellulose, previously activated by periodate oxidation, were grafted with mono-6-butylenediamino-6-deoxy-beta-cyclodextrin derivatives by reductive alkylation in the presence of sodium borohydride. The formation of supramolecular complexes between these polymers and Naproxen was confirmed by fluorescence spectroscopy. The solubility of the drug was 3.8-4.6 fold increased in the presence of the cyclodextrin-grafted polysaccharides. The in vivo anti-inflammatory property of Naproxen was 1.7 times higher after supramolecular association with beta-cyclodextrin-branched mannan.
