Efficacy of dispersed fibrous carbon sorbent in treatment of malignant fungating wounds.

AIM: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora. MATERIALS AND METHODS: The study was performed on female Wistar rats inoculated subcutaneously with Guerin T8 carcinoma into the upper interscapular region. On day 20, the tumors were infected by injecting the suspension of S. aureus 8325-4. After 24 h, gauze dressings were applied daily to the wounds in the control group and DFCS in the experimental one. The state of the wounds was assessed by visual control with photo monitoring, odor control on a verbal rating scale and microbiological analysis of qualitative and quantitative characteristics of wound microbiota. RESULTS: On the 3rd day after the start of dressings, the number of S. aureus of 8325-4 strain was significantly higher in the wounds of rats in control group than experimental one (5.14 ± 0.27 vs 3.43 ± 0.33 lg CFU/ml). The total number of Staphylococci in the DFCS-treated wounds did not differ statistically from the number of S. aureus 8325-4 strain whereas in control ones were higher by an order of magnitude. On the 10th day the total number of Staphylococci and S. aureus 8325-4 in particular, remained consistently high in the wounds of the control rats, while was almost five orders of magnitude lower and represented mainly by S. aureus 8325-4 in the experimental ones. The total number of aerobic and optionally anaerobic microorganisms was significantly lower as well. A greater variety of microorganisms in the gauze-covered wounds, as well as an their increased number were accompanied by enhancement of the wound odor from "noticeable" to "strong". There was an appearance of a "barely noticeable" odor in only one animal from the experimental group. CONCLUSION: The study has demonstrated the ability of DFCS to control substantially the bacterial microflora as well as malodor of MWFs in vivo. The results obtained can contribute to solving the problem of improving the quality of palliative care for patients with malignant and other chronic wounds.

[Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome].

Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration.

[The effect of probiotic therapy on development of experimental obesity in rats caused by monosodium glutamate].

The effect of a mixture of probiotic strains (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, Bifidobacterium animalis VKB) on the development of experimental obesity in rats induced by neonatal administration of monosodium glutamate has been studied. It was shown that in rats of 4 months age, the injection of monosodium glutamate (4 mg/g) at 2, 4, 6, 8, 10 days after birth elicited abdominal obesity and metabolic syndrome. An intermittent administration of a probiotic mixture to rats treated with monosodium prevented the development of obesity. In the group of rats treated with probiotics, anthropometric parameters (weight and body length, Lee index, body mass index) did not differ from the level of intact rats. Visceral fat mass was decreased by probiotics by 38.5% (P < 0.05) compared to rats treated with water. Probiotics improved lipid metabolism: reduced the level of VLDL by 32.2% (P < 0,05), the level of LDL by 30.6% (P < 0.05), increased HDL by 25.7% (P <0,05) compared to obese control rats. Probiotic strains restored the secretion of adipocytes hormones (leptin and adiponectin) to the normal level of intact animals. The results show the effectiveness of probiotics for the prevention of obesity.

Antistaphylococcal action of lacto- and bifidobacteria and interleukn-2.

Antistaphylococcal action of Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281, L. acidophilus LMV B-7279 and Bifidobacterium animalis VKB as well as interleukin-2 (IL-2) has been determined on the model of experimental staphylococcal infection in mice. It has been established that peroral administration of certain probiotic strains of lacto- and bifidobacteria or composition L. acidophilus LMV B-7279--L. delbrueckii subsp. bulgaricus IMV B-7281 with IL-2 to mice favored the reducing of terms of staphylococcus persistence in the kidneys of experimental mice. The studied schemes are promising for treatment of patients with surgical infection.

The effect of lacto- and bifidobacteria compositions on the vaginal microflora in cases of intravaginal staphylococcosis.

The effect of intravaginal injection of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL and Bifidobacterium animalis VKB strains in various compositions on the range of microflora of the urogenital tract on the model of experimental intravaginal staphylococcosis of mice was determined. It was established that under the influence of various compositions of these strains changes in microflora spectrum occurred: the number of representatives of normoflora increased and the number of pathogenic microorganisms, including staphylococci, streptococci, coliform bacteria and fungi, significantly decreased. It was determined that strains of lacto- and bifidobacteria, that were studied, are prospective components of future probiotic drugs efficient in treating staphylococcosis.

Antibacterial activity of cerium colloids against opportunistic microorganisms in vitro.

The CeO2 sol with the size of nanoparticles 2-4 nm has been synthesized. It has been determined that the synthesized nanocrystalline cerium has antibacterial activity in vitro against different groups of opportunistic microorganisms: clinical strains of Escherichia coli, Staphylococcus aureus and Candida albicans. The rate of viability depression of test-cultures depends on the concentration of cerium dioxide nanoparticles and time of incubation. It is shown that the sol interacts with the bacterial cell surface. It is suggested that the observed differences of antibacterial action of nanocrystalline cerium dioxide can be related to the structural characteristics of the cell surface.

Cholesterol-lowering activity of lactic acid bacteria probiotic strains in vivo.

Cholesterol-lowering activity of probiotic strains of lactic acid bacteria genera Lactobacillus and Bifidobacterium in the in vivo experiments on the model of experimental hypercholesterolemia in mice was studied. It is established that the prophylactic scheme of introduction of probiotic cultures is more effective than therapeutic one for the manifestation of cholesteraze activity of probiotic cultures. The most effective were the cultures: L. acidophilus and B. bifidum, as well as the composition B. bifidum + B. longum. Cholesterol-lowering activity of the studied strains and their compositions in this experiment ranged between 40-78%. It is noted that cholesteraze activity of other studied strains was not lower and in some cases, higher than that of most of the drugs currently used in cholesterinozis.

The effect of lacto- and bifidobacteria compositions on the vaginal microflora in cases of intravaginal staphylococcosis.

The effect of intravaginal injection of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL and Bifidobacterium animalis VKB strains in various compositions on the range of microflora of the urogenital tract on the model of experimental intravaginal staphylococcosis of mice was determined. It was established that under the influence of various compositions of these strains changes in microflora spectrum occurred the number of representatives of normoflora increased and the number of pathogenic microorganisms, including staphylococci, streptococci, coliform bacteria and fungi, significantly decreased. It was determined that strains of lacto- and bifidobacteria, that were studied, are prospective components of future probiotic drugs efficient in treating staphylococcosis.

Effect of probiotic strains of lacto- and bifidobacteria on the activity of macrophages and other parameters of immunity in cases of staphylococcosis.

The immunomodulatory properties of Lactobacillus delbrueckii subsp. bulgaricus IMVB-7281, Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animales VKL and B. animales VKB strains on the models of experimental staphylococcosis infection in mice were determined. It was found that after the mice, infected with staphylococcus, were treated by some probiotic strains of lacto- and bifidobacteria, a normalization of functional activity of phagoctic cells system and increase of the endogenous interferon production were observed. L. delbrueckii subsp. bulgaricus IMVB-7281, L. casei IMVB-7280, L. acidophilus IMV B-7279, B. animales VKB and B. animales VKL are promising for the development of probiotics, effective against staphylococci and for the immunity correction.

[Antioxidant and cerebroprotective action of N-,S-chinazolone derivative under rat brain ischemia].

Under modelling of brain ischemia in rats the antioxidant and cerebroprotective action of new N-,S-chinazolone derivative--compound NC-224 has been established. The compensation of pathobiochemical abnormalities in the system "LPO-antioxidant protection" is observed, indicators of carbohydrate metabolism and energy-supply, and morpho-functional status of brain cells are improved under administration of the compound NC-224. Administration of the compound NC-224 to rats promoted a decrease of the lethality index and signs of neurological deficiency.

[Interrelation between antioxidant hepatoprotective action of some derivatives of monochromanes and trimethylphenols and energy structural quantum-chemical parameters].

Energy and structural parameters of monochromanes and trimethylphenols derivatives have been chracterized through the employment of semiempirical methods of quantum chemistry. The correlation has been proved between antiradical, hepatoprotective properties of these compounds and energy and structural of their molecules that confirms the presence of relation between structure activity and electron-donor properties of the investigated monochromanes and trimethylphenols.

[The influence of N-, S-containing chinasolone derivatives (NC-224) on the biochemical and physicochemical parameters of membrane endoplasmatic reticulum and nuclear chromatine fractions of rats liver cells in conditions of its injury by tetrachloromethane].

Using biochemical and physicochemical methods of investigation in vivo, the effect of the substance NC-224, N-, S-chinasolone-derivative, on the lipoperoxidation activity in rat liver endoplasmatic reticulum membranes and nuclear chromatin fractions under tetrachloromethane intoxication have been studied. It was shown that NC-224 has pronounced antioxidant activity which is the biochemical basis of the substance membrane- and genome-protective effects and its ability to restore physicochemical properties of the surface and hydrophobic zones of hepatocyte membranes and structural parameter nuclear chromatin fractions in the conditions of chemical liver injury.

[Antioxidant activity of condensed [1,2,4]-triazinone derivatives in nitrosative stress].

Antioxidant activity of nine [1,2,4]-triazinone derivatives was studied in the work. Our data show that [1,2,4]-triazinone derivatives with benzyl alcohol, propyl alcohol, me-thyl alcohol and tolyl residues in their structure have antioxidant activity in condition of in vitro NO formation. KO-17 compound proved to have the greatest antioxidant activity which exceeded N-acetyl cysteine's one.