ABSTRACT
Hydrolysis data for Bis(4-cyanophenyl) phenyl phosphate (CPP), introduced as a reactive diluent for phthalonitrile monomers, under pH 4, 7 and 10 are presented. Conversion/time plots collected by HPLC analysis, typical chromatograms and NMR spectra of the substrate and the reaction products are given. Pseudo-first order rate constants are determined for CPP at 25, 50 and 80 °C. Activation parameters were calculated from Arrhenius equation.
ABSTRACT
We studied association of Oprm1 gene polymorphisms with signs of N-(1-phenethyl-4-piperidyl)propionanilide intoxication in rats. It was found that the rate of intoxication in laboratory animals depends on genetic features. A polymorphic variant rs105312806 of Oprm1 gene can be a possible marker of animal sensitivity to opioid receptor agonists. This hypothesis was supported by differences in the rats of intoxication signs such as time to lateral posture and sleep duration in homozygous rats carrying different alleles. In rats with AA genotype, the time to lateral posture was shorter by 1.3 times and sleep duration was longer by 3.5 times than in carriers of GG genotype.
Subject(s)
Hypnotics and Sedatives/pharmacology , Polymorphism, Single Nucleotide/genetics , Receptors, Opioid, mu/genetics , Alleles , Animals , Genotype , Male , Rats , Receptors, Opioid, mu/antagonists & inhibitors , Receptors, Opioid, mu/metabolism , Sleep/drug effectsABSTRACT
Expression of genes encoding the individual subunits of ionotropic GABAA receptor was assessed after acute and chronic intoxication of rats with ethanol. The chronic 1-month-long exposure to ethanol signifi cantly decreased (by 38%) expression of Gabrb1 gene in the hippocampus. Acute exposure to ethanol elevated expression of genes Gabrb1 (by 1.7 times), Gabra1 (by 3.8 times), and Gabra4 (by 6.5 times), although it diminished expression of Gabra2 gene by 1.4 times. In preliminarily alcoholized rats, acute intoxication with ethanol enhanced expression of genes Gabrb1 and Gabra5 by 1.7 and 8.7 times, respectively. There was neither acute nor chronic effect of ethanol on expression of gene Gabra3.
Subject(s)
Ethanol/adverse effects , Gene Expression Regulation/drug effects , Gene Expression/drug effects , Receptors, GABA-A/biosynthesis , Alcoholic Intoxication/metabolism , Animals , Hippocampus/metabolism , Male , Rats , Receptors, GABA-A/geneticsABSTRACT
The borreliosis etiology was verified in 60% cases of the morphea, 100% cases of the atrophoderma of Pasini-Pierini and 100% cases of anetoderma by means of IFA with Ip-21 antigen (B. afzelii) and silver staining after Levadity. Borreliae were located near the capillaries, muscular and areolar tissue fibers of the derm and near the granulated cells of epidermis.
Subject(s)
Arachnid Vectors , Ixodes , Lyme Disease/pathology , Silver Staining/methods , Skin/pathology , Animals , Biopsy , Fluorescent Antibody Technique, Indirect , HumansABSTRACT
Experimental and theoretical models of closed "autotroph-heterotroph" (chlorella-yeast, chlorella-protozoa) ecosystems with spatially separated components have been created and studied. The chart of flows and interaction of components of gas-closed "chlorella-yeast" system have formed the basis describe mathematically the functioning of the given system, experimental results have been found to agree with computer solution of the problem in terms of quality. Investigation of the experimental model of the "producer-consumer" trophic chain demonstrated the role of protozoa in nitrogen turnover. "Production-decomposition" and "production-grazing-decomposition" cycle models has been theoretically analyzed and compared. The predator has been shown to be a more intensive mineralizer than the reducer component.
