ABSTRACT
The analysis of long-term results of treatment of 88 primary patients with colon adenocarcinoma at various stages of tumor process is presented, taking into account the TNM system criteria, and serum IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, VEGF, and MMP-7 levels. The overall survival rate assessed by Kaplan-Meier method and Cox multivariate regression model was used as the criterion of prognosis. It was established that IGF-1, IGFBP-2 and VEGF serum levels along with the stage of colorectal cancer might be considered as statistically significant independent predictors of overall survival in patients.
Subject(s)
Colorectal Neoplasms , Matrix Metalloproteinase 7 , Carrier Proteins , Humans , Insulin-Like Growth Factor I/metabolism , Prognosis , Vascular Endothelial Growth Factor AABSTRACT
The content of components of the RANK/RANKL/OPG system, the key regulator of homeostasis in the bone tissue, in blood serum samples from 199 patients with primary bone neoplasms and 131 practically healthy volunteers was measured by ELISA. Borderline giantcell tumor of the bone with high osteoclastogenic and osteolytic activity is characterized by an increase in the level of all components of this system and highest ratio of sRANKL/OPG in the blood serum. Study indexes in patients with various benign neoplasms and tumor-like bone lesions were lower than in patients with giant-cell tumor. The patients with malignant bone tumors could be divided into 2 subgroups with opposite indexes of the RANK/RANKL/OPG system. The patients with osteosarcoma and Ewing sarcoma had a low level of sRANK, but a high level of sRANKL. The patients with chondrosarcoma and chordoma had a high level of sRANK, but a low level of sRANKL.
Subject(s)
Bone Neoplasms/metabolism , RANK Ligand/metabolism , Receptor Activator of Nuclear Factor-kappa B/metabolism , Adolescent , Adult , Aged , Child , Child, Preschool , Chondrosarcoma/metabolism , Chordoma/metabolism , Enzyme-Linked Immunosorbent Assay , Female , Healthy Volunteers , Humans , Male , Middle Aged , Osteoprotegerin/metabolism , Young AdultABSTRACT
It is proven that angiogenesis and cartilage resorption processes involving vascular endothelial growth factor (VEGF) and its receptors play an important role in bone tissue regeneration and bone tumor development. A comparative analysis of VEGE VEGF-R1, VEGF-R2 and angiogenin content in blood serum of healthy persons, and patients with primary malignant, benign and borderline bone tumors was performed. 366 bone tumor patients aged 14-76 years were examined; 223 (60,9%) male and 143 (39,1%) -female. Control group included 48 practically healthy subjects aged 15-69 years; 25 (52,1%) male and 23 (47,9%) female. Serum VEGE VEGF-R1, VEGF-R2 and angiogenin concentrations measured using standard ELISA kits produced by "R&D Systems Inc. ", USA. It was shown that long-term treatment outcome (3-years overall survival) of typical osteosarcoma patients depended on tumor malignancy grade (determined by the criterion G). Baseline serum VEGF, its soluble receptors and angiogenin concentrations had no predictive value.
Subject(s)
Bone Neoplasms/blood , Osteosarcoma/blood , Ribonuclease, Pancreatic/blood , Vascular Endothelial Growth Factor A/blood , Vascular Endothelial Growth Factor Receptor-1/blood , Vascular Endothelial Growth Factor Receptor-2/blood , Adolescent , Adult , Aged , Case-Control Studies , Female , Humans , Male , Middle AgedABSTRACT
Comparative enzyme immunoassay of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitor-1 (TIMP-1) in the sera of 26 healthy individuals and 54 patients with primary osteal tumors before therapy revealed elevated TIMP-1 levels in the patients with classical central and periosteal osteosarcomas in comparison with the control. In patients, the level of MMP-9 significantly decreased compared to that in healthy individuals, while the levels of MMP-2 and MMP-7 remained unchanged. No differences in serum levels of MMP and TIMP-1 associated with gender, age, primary osteal tumor location and size were detected. Overall 3-year survival of patients with classical central osteosarcoma with serum level of MMP-9 below its median was higher than that of patients with MMP-9 level equal to above the median (90.9 ± 8.7 and 50.8 ± 23%, respectively).
Subject(s)
Bone Neoplasms/blood , Matrix Metalloproteinase 2/blood , Matrix Metalloproteinase 7/blood , Matrix Metalloproteinase 9/blood , Tissue Inhibitor of Metalloproteinase-1/blood , Adolescent , Adult , Female , Humans , Immunoenzyme Techniques , Male , Middle Aged , Statistics, Nonparametric , Survival AnalysisABSTRACT
Serum levels of endostatin, placental growth factor (PlGF), and fibroblast growth factors-1 and -2 (FGF-1 and FGF-2) were measured in 58 patients with primary osteosarcomas before therapy and in 21 healthy subjects. The incidence of serum FGF-1 in bone tumors was 2.5 times higher than in healthy individuals (p=0.004); significant levels of FGF-2, PlGF, and endostatin were detected in all examined subjects. The mean serum level of endostatin in healthy individuals was significantly lower than in the total group of patients with bone tumors (p=0.005). The level of FGF-1 in osteosarcomas was significantly higher than in chondrosarcomas (p<0.05). No appreciable differences in FGF-2 levels were detected in patients with tumors of different histological structure. The mean serum content of PlGF was virtually the same in healthy individuals and patients with bone tumors. A significant relationship between serum PlGF level and maximum tumor size (p=0.008) was detected in osteosarcoma. No relationships between the levels of FGF-1, FGF-2, PlGF, and endostatin were detected in healthy subjects and patients with primary tumors of the bones. Differences in 3-year overall survival values of patients with bone sarcomas with different initial serum levels of FGF-1 and endostatin were detected.
Subject(s)
Endostatins/blood , Fibroblast Growth Factor 1/blood , Fibroblast Growth Factor 2/blood , Osteosarcoma/blood , Pregnancy Proteins/blood , Adolescent , Adult , Humans , Middle Aged , Placenta Growth Factor , Young AdultABSTRACT
We studied the dependence of serum cytotoxic activity on the contents of soluble apoptosis receptor and its soluble ligand in patients with autoimmune thyroid diseases.
Subject(s)
Autoimmune Diseases/immunology , Membrane Glycoproteins/blood , T-Lymphocytes, Cytotoxic/physiology , Thyroiditis, Autoimmune/immunology , fas Receptor/physiology , Apoptosis , Autoimmune Diseases/pathology , Case-Control Studies , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Fas Ligand Protein , Female , Goiter, Nodular/immunology , Goiter, Nodular/pathology , Humans , Ligands , Male , Russia , Thyroiditis, Autoimmune/pathologyABSTRACT
The content of urokinase- and tissue-type plasminogen activators and plasminogen activator inhibitor PAI-1 in the cytosol of primary and metastatic melanomas and benign skin pigment neoplasms was estimated by enzyme immunoassay. It was shown that local growth and invasion of melanomas are related to suppressed expression of tissue plasminogen activator. The content of urokinase plasminogen activator increases in patients with distant metastases and large thickness of the primary tumor.
Subject(s)
Melanoma/metabolism , Plasminogen Activator Inhibitor 1/metabolism , Skin Neoplasms/metabolism , Tissue Plasminogen Activator/metabolism , Urokinase-Type Plasminogen Activator/metabolism , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Melanoma/enzymology , Melanoma/pathology , Melanoma/secondary , Middle Aged , Serine Proteinase Inhibitors/metabolism , Skin Neoplasms/enzymology , Skin Neoplasms/pathology , Statistics as TopicABSTRACT
This paper presents results of comparative enzyme immunoassay of soluble serum Fas antigen (sFas) in 98 patients with benign and malignant bone tumors, 15 patients with mechanical bone traumas, and 60 normal individuals of compatible age. The prevalence and concentration of sFas were significantly greater in patients with bone tumors than in normal donors and patients with mechanical bone traumas. The association of sFas with the pathogenesis of primary bone tumors is discussed in the paper.
Subject(s)
Biomarkers, Tumor/blood , Bone Neoplasms/metabolism , fas Receptor/blood , Adolescent , Adult , Bone Neoplasms/pathology , Female , Humans , Immunoenzyme Techniques , Male , Middle Aged , Neoplasm Metastasis , SolubilityABSTRACT
Serum contents of leptin and soluble Fas antigen were measured in 28 patients with osteosarcoma, 7 with neuroectodermal bone tumors, and 17 healthy subjects. The incidence and levels of soluble Fas antigen in patients with osteosarcoma and neuroectodermal bone tumors were higher than in healthy subjects and did not depend on sex and age in both healthy subjects and patients. Serum concentration of leptin in women was higher than in men (both in healthy controls and patients) and was lower in healthy subjects compared to patients with osteosarcoma and neuroectodermal bone tumors. A trend to a negative correlation between the concentrations of leptin and soluble Fas antigen in female patients with osteosarcoma and male patients with neuroectodermal bone tumors was revealed. The role of leptin and soluble Fas antigen in the pathogenesis of primary bone tumors is discussed.
Subject(s)
Bone Neoplasms/blood , Leptin/blood , Neuroectodermal Tumors/blood , Osteosarcoma/blood , fas Receptor/blood , Adolescent , Adult , Apoptosis , Bone Neoplasms/pathology , Female , Humans , Male , Neuroectodermal Tumors/pathology , Osteosarcoma/pathology , Receptors, LeptinABSTRACT
The concentrations of vascular endothelium growth factor and angiogenin were measured by enzyme immunoassay in the sera of healthy subjects and patients with osteosarcoma and Ewing's tumor with consideration for the main clinical and morphological characteristics of the diseases. The studied angiogenic factors were characterized by high individual variability in both healthy subjects and age- and sex-matched patients with primary bone sarcomas. The level of endothelial growth factor was higher in control women than in men. The highest concentration of endothelial growth factor was detected in male patients with Ewing's tumor. Possible involvement of vascular endothelium growth factor and angiogenin in the pathogenesis of osteosarcoma and Ewing's tumor is discussed.
