All optical modulation in vertically coupled indium tin oxide ring resonator employing epsilon near zero state.

We present an innovative approach to achieve all-optical modulation within an ITO-based vertically coupled ring resonator. This method leverages the material's enhanced nonlinear response in the near-infrared wavelengths, particularly within the epsilon-near-zero (ENZ) state. To enhance the interaction between light and the material while minimizing scattering losses, our approach employs an ITO-based vertically connected ring resonator. The vertical arrangement eliminates the need for etching fine gaps to separate the ring and bus waveguide. The novel waveguide design addresses the necessity of high sensitivity, non-linear effects and compact size opening the possibilities for all-optical signal processing. This unique resonator structure effectively facilitates the coupling of a high-intensity pump wavelength into the ITO-based micro-ring resonator. Consequently, this optical pumping induces electron heating within the ITO material, leading to a significant increase in its nonlinear optical properties. This, in turn, results in a noteworthy alteration of ITO's refractive index, specifically in the unity order, thereby modifying the complex effective index of the optical beam propagating at 1550 nm. Our experimental findings demonstrate an impressive extinction ratio of 18 dB for a 30 µm long device, which highlights the efficiency of our approach in achieving all-optical modulation through the optical pumping of an ITO-based vertically coupled ring resonator. The proposed all-optical modulator has outperformed as compared to conventional waveguide-based modulators in terms of extinction ratio and footprint. This novel technique holds immense potential for advancing high-speed data communication systems in the future. As the demand for advanced processing capabilities, such as artificial intelligence, continues to grow, all-optical modulation emerges as a groundbreaking technology poised to revolutionize the next generation of computing and communication systems.

Homophilic and heterophilic cadherin bond rupture forces in homo- or hetero-cellular systems measured by AFM-based single-cell force spectroscopy.

Cadherins enable intercellular adherens junctions to withstand tensile forces in tissues, e.g. generated by intracellular actomyosin contraction. In-vitro single molecule force spectroscopy experiments can reveal cadherin-cadherin extracellular region binding dynamics such as bond formation and strength. However, characterization of cadherin-presenting cell homophilic and heterophilic binding in the proteins' native conformational and functional states in living cells has rarely been done. Here, we used atomic force microscopy (AFM) based single-cell force spectroscopy (SCFS) to measure rupture forces of homophilic and heterophilic bond formation of N- (neural), OB- (osteoblast) and E- (epithelial) cadherins in living fibroblast and epithelial cells in homo- and hetero-cellular arrangements, i.e., between cells and cadherins of the same and different types. In addition, we used indirect immunofluorescence labelling to study and correlate the expression of these cadherins in intercellular adherens junctions. We showed that N/N and E/E-cadherin homophilic binding events are stronger than N/OB heterophilic binding events. Disassembly of intracellular actin filaments affects the cadherin bond rupture forces suggesting a contribution of actin filaments in cadherin extracellular binding. Inactivation of myosin did not affect the cadherin rupture force in both homo- and hetero-cellular arrangements, but particularly strengthened the N/OB heterophilic bond and reinforced the other cadherins' homophilic bonds.

Nano-mechanical mapping of interdependent cell and ECM mechanics by AFM force spectroscopy.

Extracellular matrix (ECM), as a dynamic component of the tissue, influences cell behavior and plays an important role in cell mechanics and tissue homeostasis. Reciprocally, this three-dimensional scaffold is dynamically, structurally and mechanically modified by cells. In the field of biophysics, the independent role of cell and ECM mechanics has been largely investigated; however, there is a lack of experimental data reporting the interdependent interplay between cell and ECM mechanics, measured simultaneously. Here, using Atomic Force Microscopy (AFM) we have characterized five different decellularized matrices diverse in their topography, ECM composition and stiffness and cultured them with normal and pathological fibroblasts (scar and Dupuytren's). We investigated the change in topography and elasticity of these matrices due to cell seeding, by using AFM peak force imaging and mechanical mapping, respectively. We found normal fibroblasts soften these matrices more than pathological fibroblasts, suggesting that pathological fibroblasts are profoundly influencing tissue stiffening in fibrosis. We detected different ECM composition of decellularized matrices used here influences fibroblast stiffness, thus highlighting that cell mechanics not only depends on ECM stiffness but also on their composition. We used confocal microscopy to assess fibroblasts invasion and found pathological fibroblasts were invading the matrices deeper than normal fibroblasts.

Mechanics of Brain Tissues Studied by Atomic Force Microscopy: A Perspective.

Tissue morphology and mechanics are crucial to the regulation of organ function. Investigating the exceptionally complex tissue of the brain at the sub-micron scale is challenging due to the complex structure and softness of this tissue, despite the large interest of biologists, medical engineers, biophysicists, and others in this topic. Atomic force microscopy (AFM) both as an imaging and as a mechanical tool provides an excellent opportunity to study soft biological samples such as live brain tissues. Here we review the principles of AFM, the performance of AFM in tissue imaging and mechanical mapping of cells and tissues, and finally opening the prospects and challenges of probing the biophysical properties of brain tissue using AFM.

Mechanical and migratory properties of normal, scar, and Dupuytren's fibroblasts.

Mechanical properties of myofibroblasts play a key role in Dupuytren's disease. Here, we used atomic force microscopy to measure the viscoelastic properties of 3 different types of human primary fibroblasts derived from a same patient: normal and scar dermal fibroblasts and palmar fascial fibroblasts from Dupuytren's nodules. Different stiffness hydrogels (soft ~1 kPa and stiff ~ 50 kPa) were used as cell culture matrix to mimic the mechanical properties of the natural tissues, and atomic force microscopy step response force curves were used to discriminate between elastic and viscous properties of cells. Since transforming growth factor-ß1 (TGF-ß1) is known to induce expression of α-smooth muscle actin positive stress fibers in myofibroblasts, we investigated the behavior of these fibroblasts before and after applying TGF-ß1. Finally, we performed an in vitro cell motility test, the wound healing or scratch assay, to evaluate the migratory properties of these fibroblasts. We found that (1) Dupuytren's fibroblasts are stiffer than normal and scar fibroblasts, the elastic modulus E ranging from 4.4, 2.1, to 1.8 kPa, for Dupuytren's, normal and scar fibroblasts, respectively; (2) TGF-ß1 enhances the level of α-smooth muscle actin expression and thus cell stiffness in Dupuytren's fibroblasts (E, ~6.2 kPa); (3) matrix stiffness influences cell mechanical properties most prominently in Dupuytren's fibroblasts; and (4) Dupuytren's fibroblasts migrate slower than the other fibroblasts by a factor of 3. Taking together, our results showed that mechanical and migratory properties of fibroblasts might help to discriminate between different pathological conditions, helping to identify and recognize specific cell phenotypes.

Preconcentrative separation of palladium(II) using palladium(II) ion-imprinted polymer particles formed with different quinoline derivatives and evaluation of binding parameters based on adsorption isotherm models.

Palladium(II) ion-imprinted polymer (IIP) materials were synthesized by thermally polymerizing the ternary complexes of palladium(II) with amino (AQ) or hydroxy (HQ) or mercapto (MQ) derivatives of quinoline and 4-vinyl-pyridine. The functional and crosslinking monomers used during polymerization were 2-hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA). 2,2'-Azobisisobutyronitrile (AIBN) and 2-methoxy ethanol were used as the initiator and porogen, respectively. The resulting polymer materials were dried in an oven at 80 degrees C, ground and sieved to obtain IIP particles which were then subjected to leaching with 50% (v/v) HCl to obtain the leached palladium(II) IIP particles. Control polymer (CP) particles were also prepared by following the above procedure described for IIP particles. The CP particles, unleached and leached AQ-based IIP particles were then characterized by IR, XRD and microanalysis studies. Analytical studies such as preconcentration of palladium(II) from dilute aqueous solutions and separation studies in the presence of selected noble and base metals which co-exist with palladium(II) in its ore or mineral deposits were systematically studied using CP and IIP particles and are compared. AQ-based IIP particles gave higher percent extraction and selectivity coefficients compared to HQ- or MQ-based IIP particles. Five replicate determinations of 25mug of palladium(II) present in 500ml of aqueous solution, when subjected to preconcentration and determination by iodide-Rhodamine 6G procedure gave a mean absorbance of 0.104 with a relative standard deviation of 2.25%. The detection limit corresponding to three times the standard deviation of the blank was found to be 5.0mug of palladium(II) per litre. The rebinding studies using AQ-, HQ- and MQ-based IIPs were carried out and were fitted to the different adsorption isotherm models, viz. Langmuir (L), Freundlich (F) and Langmuir-Freundlich (LF). These adsorption models were used for the evaluation of binding parameters and in elucidating the nature and type of bonding in the IIPs. The results of rebinding experiments showed discrimination between the three IIPs based on the donor atoms of the ligands.