Screening of antimicrobials, fragrances, UV stabilizers, plasticizers and preservatives in sewage treatment plants (STPs) and their risk assessment in India.

Community/industrial wastewater is the prime source of anthropogenic chemicals, its treatment is often a daunting task and unaffordable for many countries. Emerging Contaminants (ECs) have been drained into wastewater after continuous use/misuse and Conventional treatments in STPs do not remove them completely. ECs including antimicrobial agents, synthetic musks, Benzotriazole UV stabilizers (BUVSs), plasticizers, and preservatives are frequently reported in environment, and cause health effects to non-target organisms. Monitoring of ECs is important to understand their status in aquatic environment. Hence, it was aimed to monitor ECs (n = 21) from 11 STPs in Tamil Nadu, India. The detection frequency of most of these analytes was >90%. Antimicrobials ranged from 247 to 22,714 ng/L and 11-14,369 ng/L in influents and effluents, respectively. The synthetic musks were in the order of Tonalide > Galaxolide > Musk Ketone. BUVSs ranged from 4 to 1632 ng/L (influents) and < LOD to 29,853 ng/L (effluents). Concentration of phthalates in influents and effluents were < LOD - 11,311 ng/L and < LOD - 17,618 ng/L, respectively. Parabens were found in the order of Prophyl > Methyl > Ethyl > Butyl in influents and Methyl > Prophyl > Butyl > Ethyl in effluents. Mass loads of ECs through STPs were found as antimicrobials > plasticizers > fragrances > BUVSs > Preservatives. This study reveals increasing usage of ECs and inadequate treatment processes at STPs in India. Also helps to adopt suitable treatment processes to remove ECs from wastewater and to reuse the wastewater.

Urinary concentration of endocrine-disrupting phthalates and breast cancer risk in Indian women: A case-control study with a focus on mutations in phthalate-responsive genes.

BACKGROUND: Phthalates are known endocrine-disrupting chemicals used indiscriminately as constituents in consumer products including food processing, and packaging, cosmetics, personal care and household items. Although, few studies have assessed the risk of breast cancer on exposure to phthalates, their association with breast cancer risk in Indian women have not yet been evaluated. METHODS: We conducted a case-control study involving 171 participants. Urinary concentrations of six phthalate dieters; DMP (Dimethyl phthalate), DEP (Diethyl phthalate), DBP (Dibutyl phthalate), BBP (benzyl butyl phthalate), DEHP (Di-2-ethyl-hexyl phthalate), DINOP (Di-n-octyl phthalate) were estimated by GC-MS and geometric means were calculated. Univariate and multivariable logistic regression was performed to assess breast cancer risk on exposure to phthalates. Genes responsive to phthalates were identified through literature search and matched with NGS data, and gene-enrichment analysis was performed. RESULTS: Significant associations were observed between urinary phthalate concentrations and increased risk of breast cancer for di-butyl phthalate (OR=1.5, 95% CI; 1.06, 2.11, p = 0.002) and di-2-ethyl-hexyl phthalate (>median vs ≤ median; OR=2.97, 95% CI; 1.18, 7.47, p = 0.005) in multivariable analyses. We also found several phthalate-responsive gene mutations in paired breast tumor tissues, which include PTPRD (76.19%), AR (42.86%), CYP1A1 (42.86%), CYP19A1 (23.81%), AHRR (19.05%), PIK3CA (19.05%), CYP1B1 (9.52%), RB1 (9.52%) and MMP9 (9.52%). Gene-enrichment analysis revealed that these genes form a major part of ER/PR, PPAR and HIF-1α-TGF-ß signaling cascades involved in breast cancer CONCLUSION: Although the sample size is small, in this first case-control study from India, DBP and DEHP were found to be associated with increased risk of invasive breast cancer and tumor tissues revealed mutations in several phthalate-responsive genes. It is, therefore suggested that human biomonitoring in India and larger studies evaluating the early life genetic and epigenetic alterations on phthalates exposure are required to establish their role in breast carcinogenesis.

Synthetic musks in surface water and fish from the rivers in India: Seasonal distribution and toxicological risk assessment.

Synthetic musks (SMs), a class of organic compounds added to various personal care products (PCPs) to enhance aroma, are increasingly released into the environment and become one emerging contaminants of concern in India. Some SMs like Galaxolide, Tonalide and Musk Ketone (MK) are lipophilic and found ubiquitously in the environment, posing health and ecological risks, especially affecting aquatic organisms. Hence, monitoring the synthetic musks contamination in these rivers become environmentally inevitable. Consequently, three major rivers, the Kaveri (Cauvery), Vellar and Thamirabarani Rivers in Tamil Nadu, India, were investigated to understand the occurrence and fate of SMs. The concentration of Galaxolide, Tonalide and MK in surface water ranged as not detected (ND)-198, ND-77 and ND-62 ng/L, respectively. The levels of SMs in the Kaveri River were comparable with Vellar and Thamirabarani Rivers; however, the detection frequency was low in Thamirabarani river. Fish samples from the Kaveri river had higher concentrations of SMs (galaxolide 36-350 ng/g > MK 2-33 ng/g > Tonalide 1-9 ng/g ww (wet weight)) than in the Vellar River. Based on Hazard Quotient, SMs pose no risks to freshwater systems and the resident organisms in this study. In India, the dry season starts from March to July (35-42 °C) and wet season starts from November to February (25-35 °C). Bioconcentration factor (BCF) values for Galaxolide were found higher during the wet season and lower during the dry season, whereas it is reverse for Tonalide. Among fish Gebilion catla may be a good indicator species for SMs, despite the seasons, it accumulates more. This is the first study of SMs in surface water and fish from the rivers in India.

Occurrence of triclocarban and benzotriazole ultraviolet stabilizers in water, sediment, and fish from Indian rivers.

Triclocarban and benzotriazole ultraviolet stabilizers (BUVSs) are listed as high production volume synthetic chemicals, used extensively in personal care products. Many of these chemicals persist in the aquatic environment as micropollutants. Knowledge on their fate in freshwater ecosystems is still lacking, especially in the Indian Rivers. Our intention is to study the seasonal distribution, hazard quotient, risk assessment, and bioaccumulation of triclocarban and BUVSs (UV-9, UV-P, UV-326, UV-327, UV-328, and UV-329) during wet and dry seasons in water, sediment and fish from the Kaveri, Vellar, and Thamiraparani rivers in Tamil Nadu State, India. Triclocarban and BUVSs were identified in all matrices analysed. Triclocarban was found in water, sediment, and fish up to 1119ng/L, 26.3ng/g (dry wt.), and 692ng/g (wet wt.), respectively. Among BUVSs, UV-329 was found up to 31.3ng/L (water samples), UV-327 up to 7.3ng/g (sediment samples), and UV-9 up to 79.4ng/g (fish samples). The hazard quotient (HQenv.) for triclocarban in surface water was found to be at risk level (HQenv. >1) in the Kaveri, and Thamiraparani rivers during dry season. Bioaccumulation factors indicate that target compounds (triclocarban and BUVSs) could bio-accumulate in organisms.

GC-MS determination of phthalate esters in human urine: A potential biomarker for phthalate bio-monitoring.

Six phthalic acid esters (PAEs) in human urine sampled randomly from three districts (Erode, Thanjavur, and Perambalur) in Tamil Nadu State and a Union Territory (Pondicherry) in India were quantified. We determined gender-wise, age-wise and location-wise distribution of PAEs and measured estrogenic activity of urine by molecular docking. Bis(2­ethylhexyl) phthalate (DEHP) was the predominant phthalic acid ester found and had a recovery of 104.5% (ultrasonic extraction at 15 min). Gender-wise (pregnant women: 185 ng/mL, children: 156 ng/mL, female: 151 ng/mL, and male: 138 ng/mL), age-wise (1-20 y: 157 ng/mL, 21-40 y: 156 ng/mL, and >40 y: 146 ng/mL), location-wise (urban: 154 ng/mL, and rural: 151 ng/mL), and region-wise (Erode district: 185 ng/mL, Thanjavur district: 155 ng/mL, Perambalur district: 117 ng/mL, and Pondicherry: 135 ng/mL) differences with total mean of Σ6 PAEs were found. The molecular docking study showed a high negative binding energy of PAEs with microbial receptors. Based on the results we conclude that urine could be used as an ideal biomarker to understand PAEs exposure in humans.

Detection of metal induced cytopathological alterations and DNA damage in the gills and hepatopancreas of green mussel Perna viridis from Ennore Estuary, Chennai, India.

This study report the impact of heavy metals on cytopathology and DNA damage in the gills and hepatopancreas of Perna viridis collected from Ennore estuary and the Kovalam coastal waters. Principal Component Analysis (PCA) showed significant differences among all variables at the scale of plots. The ultrastructural alterations such as lack of microvilli, distorted mitochondria, electron dense particles and the presence of large mucous droplets were common in the gill and hepatopancreatic cells of mussels from Ennore estuary. However, the gill and hepatopancreatic cells of P. viridis from Kovalam revealed normal compartmentalization of cells. The percentage of tail DNA in the mussels from Ennore estuary was recorded as 12.44 and 10.14% in the gills and hepatopancreas respectively. Overall, it has been demonstrated that the Comet and cytopathological assays are useful biomarkers to assess the level of pollution and it provide reliable information on ecotoxicology and genotoxicology of coastal waters.