Antiulcer action of the hydroalcoholic extract and fractions of Davilla rugosa Poiret in the rat.

Davilla rugosa Poiret ("Cipó- Caboclo") is commonly used in Brazilian folk medicine. The hydroalcoholic extract of the stems (HE) was fractionated with chloroform (CF), chloroform/ethyl acetate (CAF), ethyl acetate (AF), ethyl acetate/ethanol (AEF), ethanol (EF), and ethanol/water (EWF). The hydroalcoholic extract (HE) and fractions of the stems of D. rugosa Poiret were investigated for possible anti gastric ulcer properties. These extracts were shown to protect rats from developing gastric ulcers induced by two acute models: HCl/ethanol (400 mg/kg i.p.) and immersion-restraint stress (15 and 30 mg/kg of the HE and 15 mg/kg either of the ethanol or the ethanol/water fractions, p.o.). The daily oral dose of 800 mg/kg of HE for 30 consecutive days was tested for possible toxic effects. There were no modifications in body weight, water or food intake or in the external aspect of kidneys, spleen, lungs and liver. The only difference observed was a decrease of liver weight. These results suggest that the D. rugosa Poiret HE has an antiulcer activity in rats and the active components are in the two more polar fractions.

Antiulcer action of Microgramma squamulosa (Kaulf.) Sota.

Microgramma squamulosa (Kaulf.) Sota (Polypodiaceae) is commonly used as an antiulcer agent in the state of São Paulo, mainly in the upper land. The present work aims to study the antiulcer action of the crude extract of the plant rhizome and its toxicity. The effective dose was determined through acute ulcer induction test by stress. Using a determined dose, we performed a test against ulcer through acute induction by ethanol and hydrochloric acid, using cimetidine and misoprostol as reference drugs in both tests. The same extract, its ethanol and ethanol + water (1:1) fractions and the reference drug cimetidine were tested through subchronic induction test with acetic acid. The subchronic toxicity test was performed using a dose of 800 mg/kg of the crude extract, orally administered for 30 days. Finally the extracts and fractions were analysed in order to determine the main chemical groups of substances.

Antiulcer action and toxicity of Styrax camporum and Caesalpinia ferrea.

Oral administration of Styrax camporum Pohl and Caesalpinia ferrea Martius stem extracts, atropine, and cimetidine, during 15 days, decreased the ulceration size, gastric secretion volume, and increased collagen fibre number of chronic ulcer induced by acetic acid in rats. The pH increased and the concentration of hydrochloric acid decreased after administration of atropine and cimetidine. The ethyl acetate fraction, obtained by chromatography from Styrax camporum Pohl extracts, showed antiulcer activity. The subchronic toxicity was studied during 30 days, through weight variation, water and food consumption and spleen, lung, kidney and liver relative weights. No difference to the control-lot was observed.

Antiulcer action of Styrax camporum and Caesalpinia ferrea in rats. '.

Stem extracts of Styrax camporum Pohl and Caesalpinia ferrea Martius were administered orally and intraperitoneally to rats and effected a decrease in the acute ulceration number. The volume, pH, and hydrochloric acid concentration of gastric secretions were measured after administration of both extracts, atropine, and cimetidine. After oral administration there was a decrease in the gastric secretion volume without modification of the other gastric secretion parameters studied. There were modifications, however, when the extracts were administered intraperitoneally. Analgesy, influence on sleeping time, and spontaneous motor activity test results indicate a probable absence of central action components in the extracts.