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J Biomed Mater Res A ; 95(4): 1233-43, 2010 Dec 15.
Article in English | MEDLINE | ID: mdl-20939049

ABSTRACT

Five blends of poly(L-lactic acid) (PLLA) with poly(D,L-lactic-co-glycolic acid) (PLGA) were used to prepare microcarriers by a solvent evaporation technique. Microcarriers were evaluated as suitable scaffolds to facilitate retinal pigment epithelium (RPE) cell transplantation. The blend ratios were 90:10, 75:25, 50:50, 25:75, 10:90 (PLLA:PLGA). Samples of each microcarrier blend were coated with an extracellular matrix protein. Coated and uncoated microcarriers were seeded with a human RPE cell line. As the lactide unit content increased the size of microcarriers generally became larger and the surface more irregular. Cells remained proliferative and retained phenotype as confirmed by immunocytochemistry. Blends rich in PLLA were superior for maintenance of RPE cell viability. This study demonstrates for the first time the feasibility of using microcarriers as a vehicle for retinal cell transplantation for ocular disease.


Subject(s)
Biocompatible Materials/pharmacology , Esters/pharmacology , Lactic Acid/pharmacology , Microspheres , Polymers/pharmacology , Retinal Pigment Epithelium/cytology , Retinal Pigment Epithelium/transplantation , Tissue Scaffolds/chemistry , Cell Death/drug effects , Cell Line , Cell Survival/drug effects , Coated Materials, Biocompatible/pharmacology , Humans , Immunohistochemistry , In Situ Nick-End Labeling , L-Lactate Dehydrogenase/metabolism , Particle Size , Polyesters , Polyglycolic Acid/pharmacology , Polylactic Acid-Polyglycolic Acid Copolymer , Retinal Pigment Epithelium/enzymology , Retinal Pigment Epithelium/ultrastructure , Surface Properties/drug effects
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