ABSTRACT
Human decidua tissue releases immunoactive prolactin into the medium upon incubation in vitro. The prolactin secreted is indistinguishable from pituitary prolactin in its binding and displacement characteristics, using two different antisera. By gel chromatographic criteria more than 90% of the prolactin is monomeric.
Subject(s)
Decidua/metabolism , Prolactin , Chromatography, Gel , Female , Humans , Pregnancy , Prolactin/immunology , RadioimmunoassaySubject(s)
Phosphatidylethanolamines , Anticoagulants/chemical synthesis , Blood Coagulation/drug effects , Chemical Phenomena , Chemistry , Esters , Formates , Glycols/chemical synthesis , Glycols/pharmacology , Oleic Acids , Phosphatidylethanolamines/chemical synthesis , Phosphatidylethanolamines/pharmacology , Propylene Glycols , SerineABSTRACT
The phthalimidomethyl ester ofN-anisyloxycarbonyl-hydroxy-L: -proline was combined with phosphorus oxychloride andrac-1,2-diolein. The diolein was made by large-scale preparative application of the method of Krabisch and Borgström (1). The protected phosphatide, obtained by the phosphorylation reaction, was stripped of its protective groups under mild conditions. The phosphatidyl(dioleoyl)-hydroxy-L: -proline was purified by TEAE cellulose (acetate) chromatography, as developed by Rouser (6), also by silicic acid chromatography. Aqueous dispersions of the material were tested for anticoagulant activity in the antithrom-boplastin test and the Hicks-Pitney test. The new phosphatide had about one-tenth of the activity of beef brain phosphatidylserine.
ABSTRACT
An unsaturated phosphonolipid analogous to phosphatidylethanolamine,rac-dioleoylglyceryl(2-aminoethyl)phosphonate, was synthesized by a general method introduced by Baer for similar saturated substances. An improvement was made in the preparation of the phthalimidoethyl-phosphonic acid precursor.The phosphonolipid was purified by DEAE cellulose and silicic acid chromatography. It was tested by comparison with synthetic phosphatidyl (dioleoyl) ethanolamine and phosphatidyl(dilinoleoyl) ethanolamine in the Hicks-Pitney test and in a test for prothrombin conversion by using purified blood coagulation factors. In both tests it had more acceleratory activity than the synthetic phosphatidylethanolamines.
ABSTRACT
A total synthesis ofDL: -phosphatidyl-(dilinoleoyl)-L: -serine was achieved by the acylation of the barium salt of the phthalimidomethyl ester of glycerophosphoryl-N: -anisyloxycarbonyl-L: -serine. The dilinoleoyl intermediate was treated with hydrazine to remove the phthalimidomethyl group and with hydrogen chloride to remove the anisyloxycarbonyl protecting group. The resulting phosphatidylserine was purified by Rouser's methods, solubilized, and tested for biological activity in the antithromboplastin, recalcification, and Hicks-Pitney tests. It was found to have about the same anticoagulant activity as beef brain phosphatidylserine and hence was more active than the less unsaturated phosphatidylserine synthesized earlier.
