ABSTRACT
INTRODUCTION: Antimicrobial-resistant Escherichia coli and others pathogens bacteria can be transferred from animals to humans through consumption of contaminated food and foods products and thus present a public health risk. The increase in E. coli resistance to commonly used antimicrobials both in the public health and veterinary sectors is one of the major threats of health care worldwide. The present study was undertaken to estimate the antimicrobial resistance of E. coli isolates from raw chicken meat in Dakar. MATERIAL AND METHOD: Levying of skin and muscle have been carried out on 120 chicken carcasses bought from 13 sale points and 23 flocks beetween November 2003 and April 2004. 102 Escherichia coli strains have been isolated, among which, 90 were tested for their susceptibilities to 16 selected antibiotics by agar diffusion method. RESULTS: All Escherichia coli strains (100%), were resistant to one or more antibiotic; 60 strains (66.66%) being resistant to more than five antibiotics. Those frequently encountererd are: ampicillin, trimethoprim, trimethoprim-sulfametoxazole, tetracycline, sulfonamides, streptomycin, nalidixic acid. Multiple resistances to 12 antibiotics were also observed. The lowest resistances were noted with gentamicin (3.33%) and neomycin (5.56%). CONCLUSION: This study showed the significance of chicken meat as source of Escherichia coli strains with a simple or multiple resistance to various antibiotics tested. Further studies are necessary in order to determine bacterium mechanisms of resistance.
Subject(s)
Anti-Bacterial Agents/pharmacology , Drug Resistance, Multiple, Bacterial , Escherichia coli/drug effects , Meat Products/microbiology , Animals , Chickens , SenegalABSTRACT
INTRODUCTION: Antimicrobial-resistant Escherichia coli and others pathogens bacteria can be transferred from animals to humans through consumption of contaminated food and foods products and thus present a public health risk. The increase in E. coli resistance to commonly used antimicrobials both in the public health and veterinary sectors is one of the major threats of health care worldwide. The present study was undertaken to estimate the antimicrobial resistance of E. coli isolates from raw chicken meat in Dakar. MATERIAL AND METHOD: Levying of skin and muscle have been carried out on 120 chicken carcasses bought from 13 sale points and 23 flocks beetween November 2003 and April 2004. 102 Escherichia coli strains have been isolated, among which, 90 were tested for their susceptibilities to 16 selected antibiotics by agar diffusion method. RESULTS: All Escherichia coli strains (100%), were resistant to one or more antibiotic; 60 strains (66.66%) being resistant to more than five antibiotics. Those frequently encountererd are: ampicillin, trimethoprim, trimethoprim-sulfametoxazole, tetracycline, sulfonamides, streptomycin, nalidixic acid. Multiple resistances to 12 antibiotics were also observed. The lowest resistances were noted with gentamicin (3.33%) and neomycin (5.56%). CONCLUSION: This study showed the significance of chicken meat as source of Escherichia coli strains with a simple or multiple resistance to various antibiotics tested. Further studies are necessary in order to determine bacterium mechanisms of resistance.
Subject(s)
Anti-Bacterial Agents/pharmacology , Drug Resistance, Multiple, Bacterial , Escherichia coli/drug effects , Meat/microbiology , Animals , Chickens/microbiology , Escherichia coli/isolation & purification , Humans , SenegalABSTRACT
Two antigens of polysaccharidic nature were evaluated for their specificity in serodiagnosis of porcine pleuropneumonia (serotype 5). Recently, long chains lipopolysaccharides (LC-LPS) was shown to increase the specificity of the ELISA test for A. pleuropneumoniae serotype 5 compared to a saline extract of boiled-formalinized whole cells. Nevertheless, some cross reactions were noticed with LC-LPS. We have thus cleaved the LPS molecule at the ketosidic bond between the lipid A and the core. The lipid A was separated from the polysaccharide (PS) by centrifugation. With an indirect sandwich ELISA, it was possible to demonstrate that the PS yielded positive responses with homologous sera (serotype 5) and negative responses with heterologous sera (serotype 3 and undeterminated serotype). Overall, the use of the PS did not notably increase the specificity of the ELISA test. Since production of PS from LC-LPS and its separation from the lipid A is not simple, we do not recommend its use in serodiagnosis.
