Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Appl Biochem Biotechnol ; 36(3): 199-210, 1992 Sep.
Article in English | MEDLINE | ID: mdl-1288409

ABSTRACT

A biosensor to quantify L-proline within 10(-5)-10(-3) mole/L concentration is described. Immobilized Pseudomonas sp. cells grown in a medium containing L-proline as the only source of carbon and nitrogen were used to create the biosensor. The cells oxidized L-proline specifically consuming O2 and did not react with other amino acids and sugars. The change in oxygen concentration was detected with a Clark oxygen membrane electrode. The cells were immobilized by entrapment in polyvinyl alcohol (PVA) cryogel. The resultant biocatalyst had a high mechanical strength and retained its L-proline-oxidizing ability for at least two months.


Subject(s)
Biosensing Techniques , Proline/analysis , Pseudomonas/chemistry , Adenosine Triphosphate/analysis , Bacteriological Techniques/instrumentation , Culture Media , Oxygen Consumption , Pseudomonas/growth & development
SELECTION OF CITATIONS
SEARCH DETAIL
...