[Seronin syndrome and cardiac arrest caused by high-dose moclobemide (case report)].

Serotonin syndrome is the syndrome resulting from brain tissue serotonin accumulation and accompanying by central nervous system dysfunction and circulatory collapse, which leads to a serious mortal danger to life. A female patient aged 31 years, diagnosed as having chronic psychosis in the history, was admitted to an intensive care unit in a critical state for having taking an increased moclobemide dose. The patient developed cardiac arrest and cardiopulmonary resuscitation (CPR) was initiated. A 15-minute CPR recovered sinus rhythm and pulse on the peripheral arteries of the limbs. When consciousness and respiration improved, the patient was weaned from resuscitation and extubated on the second day. On day 4, the patient was transferred from the intensive care unit to the department of psychiatry. The authors consider that patients with overdosage of antipsychotic agents at a risk for such serious complications, such as cardiac arrest, should be necessarily monitored in the intensive care unit.

Functional expression in vitro of bovine visual rhodopsin.

Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. The recombinant rhodopsin has functional, spectral and immunochemical properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins, Cys316----Ser and Asp330----Asn, Asp331----Asn, were produced by oligonucleotide-directed mutagenesis. The first mutation does not affect rhodopsin's ability to stimulate transducin GTPase and visual cGMP phosphodiesterase activities, while the second double mutation leads to a sharp decrease in rhodopsin activity.

[Synthesis of visual rhodopsin in a cell-free translation system. II. Functional properties of recombinant rhodopsin and its mutant forms]. / Sintez zritel'nogo rodopsina v beskletochnoi sisteme transliatsii. II. Funktsional'nye svoistva rekombinantnogo rodopsina i ego mutantnykh form.

Functional expression of bovine visual rhodopsin in the cell-free translation system with cotranslational insertion of the protein into phosphatidylcholine liposomes is described. The recombinant rhodopsin has spectral and functional properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins with amino acid substitutions in the hydrophilic C-terminal domain were obtained using oligonucleotide-directed mutagenesis. It was found that substitution Cys-316----Ser does not affect rhodopsin's ability to activate the visual amplification cascade, whereas double mutation Asp-330----Asn, Asp-331----Asn dramatically lowers the rhodopsin functional activity.

[Use of the UKKM-1M apparatus for the cryopreservation of bone marrow and leukocytes]. / Ispol'zovanie ustanovki UKKM-1M dlia kriokonservirovanii kostnogo mozga i leikotsitov.

The make-up of an installation for programmed congelation of the bone marrow and nuclei-containing blood cells with liquid nitrogen acting the part of a refrigerant is described. Experience gained as a result of a three-year long operation of the unit helped reveal additional conditions of its use that expand the field of its application in freezing other kinds of biological tissues and cells. An important feature of the installation includes the possibility of recording the temperature directly of the tissue undergoing congelation and not that in the freezing chamber.