ABSTRACT
Identification of a bacterial strain, designated CJ71(T), was carried out using a polyphasic taxonomic approach. Strain CJ71(T) was isolated from sediment from the estuarine wetland of the Han River, South Korea, by enrichment culture using pyrene as the sole carbon and energy source. The isolate was white-pigmented, rod-shaped, Gram-positive, strictly aerobic and motile. 16S rRNA gene sequence analysis revealed that strain CJ71(T) had the highest sequence similarity (96.9 %) to Brevibacillus formosus DSM 9885(T). The predominant cellular fatty acids in strain CJ71(T) were anteiso-C(15 : 0) (49.5 %), iso-C(15 : 0) (16.9 %), iso-C(14 : 0) (16.9 %) and iso-C(16 : 0) (4.9 %). The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 52.4 mol%. Results from the polyphasic taxonomic study suggest that strain CJ71(T) represents a novel species of the genus Brevibacillus for which the name Brevibacillus fluminis sp. nov. is proposed; the type strain is CJ71(T) (=KACC 13381(T)=JCM 15716(T)).
Subject(s)
Geologic Sediments/microbiology , Gram-Positive Endospore-Forming Rods/classification , Wetlands , Base Sequence , Fatty Acids/analysis , Gram-Positive Endospore-Forming Rods/genetics , Gram-Positive Endospore-Forming Rods/isolation & purification , Gram-Positive Endospore-Forming Rods/physiology , Molecular Sequence Data , PhylogenyABSTRACT
A novel bacterial strain designated CJ25(T) was isolated from the estuarine wetland of the Han river in Korea. Identification of this strain was carried out on the basis of polyphasic taxonomy. The isolate was Gram-staining-positive, rod-shaped, non-pigmented and motile. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to Paenibacillus chondroitinus DSM 5051(T) with 96.1 % similarity. The predominant fatty acids were anteiso-C(15 : 0) (50.25 %), iso-C(16 : 0) (18.54 %) and iso-C(15 : 0) (10.00 %). The major isoprenoid quinone was MK-7. The G+C content of genomic DNA was 50 mol%. According to physiological data and 16S rRNA gene sequence analysis, the isolate was discriminated from related members of the genus Paenibacillus. Therefore, strain CJ25(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus aestuarii sp. nov. is proposed. The type strain is CJ25(T) (=KACC 13125(T) =JCM 15521(T)).
