ABSTRACT
Three compounds were isolated from the ethyl acetate soluble fraction of the methanolic extract of the leaves of Catalpa ovata (Bignoniaceae) through repeated column chromatography. We investigated the effects of these compounds on T cell-mediated responses for tumor surveillance and proliferation in U937, HL60, and Molt-4 leukemia cells. Compounds 1-3 inhibited proliferation of those cells in a dose-dependent manner. Compound 3 showed mild effect in Molt-4 cell cytotoxicity. Compound 3 enhanced gene expressions of p53 and IL-4, but decreased IL-2 and IFN-Gamma genes in Molt-4 cell. Our findings indicate that compound 3 may enhance T cell-mediated immune responses and anticancer properties.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Bignoniaceae/chemistry , Immunity, Cellular/drug effects , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , T-Lymphocytes/drug effects , Antineoplastic Agents, Phytogenic/isolation & purification , Apoptosis/drug effects , Apoptosis/genetics , Apoptosis/immunology , Cell Proliferation/drug effects , Cell Survival/drug effects , Cytokines/biosynthesis , Cytokines/immunology , Dose-Response Relationship, Drug , Enzyme-Linked Immunosorbent Assay , Gene Expression/drug effects , HeLa Cells , Humans , Immunity, Cellular/immunology , Medicine, Korean Traditional , Molecular Structure , Plant Leaves/chemistry , Reverse Transcriptase Polymerase Chain Reaction , T-Lymphocytes/immunology , U937 CellsABSTRACT
Methanol extracts of the root of Dipsacus asper Wall (Dipsacaceae) were found to exhibit apoptosis-inducing activities in U937 (human monocyte-like histiocytic) cells. Investigation of the active n-BuOH fraction led to the isolation of akebia saponin D (ASD). Structure was established by spectroscopic methods. Treatment of U937 cells with ASD induced apoptosis in a dose dependent manner. ASD exerted strong cytotoxicity against human and murine leukemia cells. It is significantly increased the subG1 cell population and expression of p53 and Bax gene. And also ASD enhanced NO production from RAW264.7 macrophage cells. Taken together, these results strongly indicate that ASD may exert apoptosis-inducing activity via induction of apoptosis through activation chiefly via the nitric oxide and apoptosis-related p53 and Bax gene expression. These data provide scientific evidence that Dipsacus asper Wall can be useful as a chemopreventive agent.
