ABSTRACT
The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAm 1), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 microM of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgene nature of plants. The analysis of seed of T1 oftransgene lines confirmed heterozygous character of inheritance of the resistance.
Subject(s)
Eleusine/drug effects , Eleusine/genetics , Herbicide Resistance/genetics , Herbicides/pharmacology , Plants, Genetically Modified , Trifluralin/pharmacology , Tubulin/genetics , Eleusine/growth & development , Genes, Plant , Genetic Vectors , Heterozygote , Transformation, GeneticABSTRACT
The data of Agrobacterium-mediated transformation of some Linum usitatissimum cultivars zoned on the territories of Belarus and Ukraine with the plasmid carrying chimeric GFP-TUA6 gene and nptII gene as selectable marker conferring resistance to kanamycin are presented in this study. Transformation was affected by a number of factors including optical density (OD600), time of inoculation of explants with Agrobacterium and co-culture conditions. Transgenic nature of obtained lines was confirmed by PCR analysis. Expression of GFP-TUA6 gene was detected with confocal laser scanning microscopy. The obtained transgenic lines can be used for further functional studies the role of microtubules in the processes of building the flax fibres and resistance to wind.
Subject(s)
Flax , Green Fluorescent Proteins/genetics , Microtubules/ultrastructure , Recombinant Fusion Proteins/genetics , Transformation, Genetic , Tubulin/genetics , Agrobacterium tumefaciens/genetics , Arabidopsis Proteins/genetics , DNA, Plant/genetics , Flax/genetics , Flax/ultrastructure , Genes, Plant , Microscopy, Confocal , Microtubules/genetics , Plants, Genetically Modified , Polymerase Chain ReactionABSTRACT
Data on selection and characterizing of finger millet Eleusine coracana (L.) Gaertn. somaclonal variants regenerated from callus culture are presented and described. Among the tested genetically stable lines the somaclonal variant SE-7 was the most interesting due to its acquired important agricultural traits, such as a higher seed and biomass yield, rapid seed germination at lower temperatures, shortening of the main plant development stages. Data analysis shows that somaclonal variability can be a source to obtain initial material for further selection of new finger millet varieties.
