ABSTRACT
Cryopreseration of erythrocytes at ultralow temperature combined with long-term storage of erythrocytes frozen in disposable polymer hemocontainers at moderately low temperature (-30 : -80 degrees C) inside freezers with cryopreservatives based on propyleneglycol and glycerin is now the most promising type of low-temperature blood bank in Russia.
Subject(s)
Blood Banks , Blood Preservation , Cryopreservation , Erythrocytes , Blood Preservation/methods , Cryopreservation/methods , Humans , RussiaABSTRACT
Cryopreservation is the method of choice in solution of the problem of the done in good time reservation of the components of blood and bone marrow for autotransfusion in patients referred to the contingent of particular risk. Freezing of autologous hemocomponents increases the infection safety and efficiency of therapy with hemocomponents. The wide introduction of using autologous hemocomponents into the everyday surgical practice and in the arsenal of means and methods of the medicine of extreme situations is an indispensable condition for increasing the effects of curative and medico-evacuational measures.
Subject(s)
Blood Banks , Blood Component Transfusion , Blood Preservation , Blood Transfusion, Autologous , Bone Marrow , Cryopreservation , Organ Preservation , Emergencies , Humans , Risk Factors , Time FactorsABSTRACT
The first Russian solution Krimolit designed for cryopreservation of platelet concentrates down -196 degrees C was clinically tested. The therapeutical efficacy of the cryopreserved platelets was evaluated on the basis of clinical and laboratory monitoring of transfusions in 20 cancer hematological patients. They were found to have the same therapeutical action as fresh cells. The introduction of cryopreserved platelets into clinical practice allows one to form stores and to transfuse autological cells. Krimolit is recommended for clinical application.
Subject(s)
Blood Platelets , Blood Preservation , Cryopreservation , Platelet Transfusion , Blood Preservation/methods , Blood Transfusion, Autologous , Cryopreservation/methods , Evaluation Studies as Topic , Humans , Solutions , Time FactorsABSTRACT
Transplantation of autologous bone marrow was performed in 62 patients with carcinoma of the thoracic portion of the esophagus for prophylactics of disturbance of hemopoiesis and severe infectious complications in the postoperative period. With the help of myelokariocytopheresis procurement of the bone marrow was made 2-3 days before starting preoperative radiotherapy. After exfusion of the bone marrow mixture erythrocytes were separated and returned completely and immediately to the patient, myelokaryocytes being frozen. The volume of the bone marrow suspension from 1 patient made up on the average from 300 to 800 ml, the amount of the bone marrow cells in it from 4.5 to 11.2.10(9). Studies of the dynamics of concentrated and volumetric parameters of blood were performed. The functional specific features of the bone marrow erythropoiesis were estimated in the sternal punctate. The use of automyelotransfusion substantially reduced the frequency of infectious complications and, in the author's opinion, it is a fairly effective method of prophylactics and treatment of postoperative anemia.
Subject(s)
Anemia/prevention & control , Blood Loss, Surgical , Bone Marrow Transplantation/methods , Erythropoiesis/physiology , Esophageal Neoplasms/surgery , Postoperative Complications/prevention & control , Anemia/etiology , Esophageal Neoplasms/blood , Humans , Postoperative Complications/etiology , Preoperative Care , Radiotherapy Dosage , Thorax , Transplantation, AutologousABSTRACT
The authors have shown hyporegeneratory character of anemia developing after operations on the lungs followed by massive blood loss which was adequately compensated by conserved homologous blood. The transfusion of the autologous bone marrow prepared before operation results in a pronounced stimulation of production of erythrocytes, contributing to the quickest recovery of the circulating erythron.
