[Transient form of serum albumin in gynecologic cancer]. / Tranzitornaia forma al'bumina krovi pri onkoginekologicheskikh zabolevaniiakh.

The frequency of diagnostic errors in examination of 3827 patients with gynecological cancer diseases accounted for 21.6 up to 35%. The conformation of serum albumin in these patients was studied by means of different biochemical assays. The determination of the amount of the transitory form of albumin by reprecipitation with trichloroacetic acid appeared the most valuable for practical uses. Structural injuries to albumin depended on the localization, pattern and stage of the disease, and features of tumor metastatic spreading. The amount of the transitory form in patients with benign tumors and tumor-like processes in the ovaries (4.5-23.5%), hydatidiform mole (25%), uterine myoma (2-12.6%) rose insignificantly (5 +/- 2% in control) and increased in patients with initial stages of malignant tumors of the ovaries and uterus (28.9-43.7%). In uterine cervix carcinoma and uterine body sarcoma (stage II and higher stages) as well as in ovarian carcinoma, stages III and IV, its amount reached 57-100%.

[Studies on influence of detergents on human serum albumin using temperature-perturbation difference spectrophotometry]. / Izuchenie vliianiia detergentov na syvorotochnyi al'bumin cheloveka metodom temperaturno-perturbatsionnoi differentsial'noi spektrofotometrii.

The temperature-perturbation difference spectra (TPDS) of human serum albumin (NSA) were studied after incubation with different synthetic detergents--anionic, cationic, amphoteric, nonionogenic--in various molar ratios. It is shown that the detergents studied have a different effect on the perturbation of tyrosine residua spectra. Under these conditions the rigidity of their environment depending on the nature and concentration of the mentioned detergents is supposed to change.

[Serum albumin fucose in cancer patients]. / Soderhzhanie fukozy al'bumina krovi u onkologicheskikh bol'nykh.

The level of blood albumin fucose in healthy subjects and cases of non-tumor pathology and benign tumors of the uterus and ovaries was found to be I nmol of fucose/10 nmol of human serum albumin or lower, whereas in patients with malignant tumors of different localizations it was 4-7 times as high. The highest levels were recorded in patients with primary hepatic cancer and hepatic metastases. The test may be used in differential diagnosis of tumor lesions of different localization as well as for assessment of tumor progression.

[Use of the physicochemical characteristics of serum albumin for diagnosing ovarian and uterine cancer]. / Ispol'zovanie fiziko-khimicheskikh kharakteristik syvorotochnogo al'bumina dlia diagnostiki raka iaichnikov i matki.

The frequency and causes of diagnostic errors are discussed on the basis of 2,099 case histories of ovarian and uterine tumors. The use of physico-chemical characteristics of serum albumin as an additional means of diagnosis of these diseases in 16 patients and 20 healthy subjects was studied. Patients revealed changes in dispersion of optical rotation of serum albumin which suggest its despiralization. Although being nonspecific, changes in the extent of despiralization vary depending on tumor advancement and gravity of patient's condition. The level of modified serum albumin is also in correlation with tumor process and the patient's state, the highest values being registered at later stages of the disease.

[Characteristics of isoelectric fractions of human serum albumin]. / Kharakteristika izoelektricheskikh fraktsii syvorotochnogo al'bumina cheloveka.

Amino acidic composition, dispersion of optic rotation, differential temperature-perturbation spectra, molecular weight and terminal amino acids are studied for certain isoelectric fractions of human serum albumin obtained by means of isoelectric focusing in the borate-polyol system. The isolated three isoelectric fractions with pI 4.7, 4.9 and 5.1 revealed no significant differences in the composition of amino acids. They also did not differ in molecular weight and N- and C-terminal amino acids. However certain differences in the conformation and number of perturbed thyrosils are detected by means of spectral methods.

[Peculiarities of blood serum albumin transport function during physical exercise]. / Osobennosti transportnoi funktsii al'bumina syvorotki krovi pri fizicheskikh nagruzkakh.

Physicochemical properties of blood serum albumin were studied in sportsmen during physical exercises. The "carbohydrate and lipid capacity' of blood serum albumin is found to increase under physical loads. Albumin is supposed to incorporate more actively into the transport of carbohydrates or lipids used as source of energy in the muscular work. No noticeable changes in the structure of albumin are found by the optical rotation dispersion technique.

[Isolation and purification of an unspecific component from human serum albumin, typical for pathology]. / Vydelenie i ochistka nespetsificheskogo komponenta syvorotochnogo al bumina cheloveka, kharakternogo dlia patologii.

Albumin from blood serum of healthy persons and from patients with various pathologies and different severity of diseases was characterized using isoelectric focusing in borate-polyol system. In all the pathologies studied a new component occurred, which had an isoelectric point at pH 5.5 and which was not found in fresh albumin preparations isolated from healthy persons. This fraction was isolated and purified. Its isoelectric point was near pH 4.5-4.6 in isoelectrofocusing with ampholines. The divergences were due to complex formation of albumin with ampholines. The modified protein corresponded immunochemically to the human blood serum albumin, did not contain polymers, had a decreased amount of alpha-helix structures as shown by dispersion of optic rotation and its molecular mass was similar to the mass of native albumin.

[Quantitative method of estimating the conformation changes in blood serum albumin]. / Kolichestvennyi metod otsenki konformatsionnykh izmenenii al'bumina syvorotki krovi.

The method of residementation with trichloracetic acid followed by dissolution in ethanol of preparations of the human serum albumin with changed conformation under conditions of pathology was suggested for determining the amount of modified albumin. The conformationally changed albumin is separated by the mentioned method and it changes to the insoluble form. According to the content of albumin in the sediment it is possible to estimate the amount of the modified part of this protein which was determined previously by the labour-consuming method of optical rotation dispersion.

[Studies of rabbit serum albumin with fatty degeneration of liver by thermal perturbation spectroscopy]. / Izuchenie Syvorotochnogo al'bumina krolikov pri zhirovoi distrofii pecheni metodom differentsial'noi temperaturno-perturbatsionnoi spektrofotometrii.

Accessibility of tyrosiles in serum albumin of healthy rabbits and those with experimental fatty degeneration of the liver was studied by the termal parturbation spectroscopy. The method determined about 50% of perturbed tyrosiles in albumin of healthy rabbits. Serum albumin of rabbits with fatty degeneration of the liver perturbed 30% of tyrosiles, that evidences for conformational transformations of the rabbit albumin in this pathology.

[Change in the optical rotatory dispersion characteristics of serum albumin of patients with appendicitis and cholecystitis]. / Izmeneniia kharakteristik dispersii opticheskogo vrascheniia syvorotochnogo al'bumina bol'nykh ostrym appenditsitom i kholetsistitom

The authors studied the optic rotation dispersion of serum albumin in patients suffering from cholecystitis and acute appendicitis. Conforming changes in these forms of pathology characterized by despiralization processes were established. A method of purification of albumin from its modified forms, possibly causing the mentioned changes in the albumin structure is suggested.