Soil microbial improvement using enriched vinasse as a new abundant waste.

This study proposes the use of vinasse, an inexpensive and readily available waste biopolymer, as a fundamental component of a waste culture medium that can enhance the effectiveness and cost-efficiency of the microbial-induced calcite precipitation (MICP) method for sustainable soil improvement. Vinasse enriched with urea, sodium caseinate, or whey protein concentrate is employed to optimize bacterial growth and urease activity of Sporosarcina pasteurii (S. pasteurii) bacterium. The best culture medium is analyzed using Taguchi design of experiments (TDOE) and statistical analysis, considering the concentration of vinasse and urea as effective parameters during growth time. To test the best culture medium for bio-treated soil, direct shear tests were performed on loose and bio-treated sand. The results demonstrate a substantial cost reduction from $0.455 to $0.005 per liter when using the new culture medium (vinasse and urea) compared to the conventional Nutrient Broth (NB) culture medium. Additionally, the new medium enhances soil shear strength, increasing the friction angle by 2.5 degrees and cohesion to 20.7 kPa compared to the conventional medium. Furthermore, the recycling of vinasse as a waste product can promote the progress of a circular economy and reduce environmental pollution. As ground improvement is essential for many construction projects, especially those that require high shear strength or are built on loose soil, this study provides a promising approach to achieving cost-effective and sustainable soil microbial improvement using enriched vinasse.

Photogrammetric Method to Determine Physical Aperture and Roughness of a Rock Fracture.

Rock discontinuities play an important role in the behavior of rock masses and have a high impact on their mechanical and hydrological properties, such as strength and permeability. The surfaces roughness and physical aperture of rock joints are vital characteristics in joint shear strength and fluid flow properties. This study presents a method to digitally measure the physical aperture of a rock fracture digitized using photogrammetry. A 50 cm × 50 cm rock sample of Kuru grey granite with a thoroughgoing fracture was digitized. The data was collected using a high-resolution digital camera and four low-cost cameras. The aperture and surface roughness were measured, and the influence of the camera type and 3D model rasterization on the measurement results was quantified. The results showed that low-cost cameras and smartphones can be used for generating 3D models for accurate measurement of physical aperture and roughness of rock fractures. However, the selection of appropriate rasterization grid interval plays a key role in accurate estimations. For measuring the physical aperture from the photogrammetric 3D models, reducing rasterization grid interval results in less scattered measurement results and a small rasterization grid interval of 0.1 mm is recommended. For roughness measurements, increasing the grid interval results in smaller measurement errors, and therefore a larger rasterization grid interval of 0.5 mm is recommended for high-resolution smartphones and 1 mm for other low-cost cameras.