ABSTRACT
Helichrysum sanguineum (L.) Kostel (H. sanguineum), a member of the Asteraceae family, has been traditionally employed for various medicinal purposes owing to its rich phytochemical composition. This study investigates the anticancer properties of various extracts of H. sanguineum (ethanol, acetonitrile, hexane, and chloroform) against breast cancer cells, shedding light on its chemical constituents and their potential therapeutic effects. In vitro assays demonstrate the profound inhibitory effects of H. sanguineum extract on human fibroblast and breast cancer cells. Furthermore, we elucidate the underlying mechanisms of action, revealing its ability to induce apoptosis and cell cycle arrest in breast cancer cells. The cytotoxicity and apoptosis outcomes in breast cancer cells varied across different extracts, yet no adverse effects were observed on healthy cells at equivalent concentrations. Furthermore, all extracts initially promoted breast cancer cell proliferation, with the chloroform extract notably reducing cancer cell proliferation even at low concentrations. GC-MS analysis identifies the major chemical constituents of the extract, including flavonoids, terpenoids, and phenolic compounds, which likely contribute to its anticancer activity. Our findings highlight the potential of H. sanguineum extract as a natural agent for breast cancer treatment and the need for further exploration of its mechanisms and clinical applications.
