Incorporation of doxorubicin and CoFe2O4 nanoparticles into the cellulose acetate phthalate / polyvinyl alcohol (core)/ polyurethane (shell) nanofibers against A549 human lung cancer during chemotherapy/hyperthermia combined method.

Cellulose acetate phthalate (CAP)/polyvinyl alcohol (PVA)/polyurethane (PU) nanofibers were synthesized by simple and coaxial electrospinning (ES) processes. Doxorubicin (DOX) and the CoFe2O4 nanoparticles were loaded into the nanofibers. The performance of the prepared nanofibers was investigated for the sustained release of DOX against A541 lung cancer cells under chemotherapy/external magnetic field (EMF) and alternating magnetic field (AMF, hyperthermia treatment) combined methods in both the in vitro and in vivo conditions. The sustained release of DOX from core-shell nanofibers containing 5 wt% cobalt ferrite was obtained within 300, 600 h, at pH of 5.5 and 7.4 without AMF and 168, 360 h, under an alternating magnetic field (AMF). More than 98.3 ± 0.2 % of A549 cancer cells were killed in the presence of core-shell nanofibers containing 100 µg DOX and 5 % cobalt ferrite nanoparticles in the presence of AMF. The flowcytometric results indicated that only 19.1 and 8.85 % cancer cells remained alive under EMF and AMF, respectively. The in vivo results revealed in stopping the growth of tumor volume and decrease in the relative tumor volume up to 0.5 were obtained using magnetic core-shell nanofibers containing 100 µg DOX and 5 % cobalt ferrite nanoparticles in the presence of EMF and AMF, respectively.

Electrospun gold nanorods/graphene oxide loaded-core-shell nanofibers for local delivery of paclitaxel against lung cancer during photo-chemotherapy method.

The combinations of photothermal therapy (PTT) and chemotherapy (CHT) have attracted increasing attention for cancer therapy. In the present study, paclitaxel as an anticancer drug and graphene oxide/gold nanorods (GO/Au NRs) were simultaneously loaded into the poly (tetramethylene ether) glycol based-polyurethane (PTMG-PU) (core)/chitosan (shell) nanofibers prepared by the coaxial electrospinning method. The potential of the synthesized nanofiber as a pH/temperature dual responsive carrier was investigated for the controlled release of paclitaxel against A549 lung cancer during PTT/CHT combined method. The synthesized core-shell nanofibers were characterized using SEM, TEM and XRD analysis. The drug encapsulation efficiency, drug release and kinetic studies were carried out. The compatibility of the synthesized core-shell nanofibers was also investigated. The cell viability of the synthesized nanofibers treated with A549 lung cancer cells was investigated under alone CHT, alone PTT and PTT/CHT method. The in vivo studies indicated that the PTT/CHT method demonstrated an optimal therapeutic effect on tumor inhibition without change in body weight. The obtained results demonstrated that the synthesized core-shell nanofibers would be used for lung cancer treatment under NIR irradiation in the future.

Fabrication of novel chitosan-g-PNVCL/ZIF-8 composite nanofibers for adsorption of Cr(VI), As(V) and phenol in a single and ternary systems.

The chitosan-grafted-poly(N-vinylcaprolactam) (chitosan-g-PNVCL) nanofibers were synthesized via electrospinning method. ZIF-8 metal-organic frameworks nanoparticles were incorporated into the nanofibers for adsorption of Cr(VI), As(V) and phenol from water. The BET, FTIR, XRD and SEM analysis were carrfried out to obtain the characteristics of nanofibers. The optimum parameters of ZIF-8 content, pH, contact time, adsorbent dosage, and initial concentration of adsorbates on the Cr(VI), As(V) and phenol removal were studied. The reusability of synthesized nanofibers for five sorption-desorption cycles was also examined. The maximum experimental adsorption capacity of the chitosan-g-PNVCL/ZIF-8 nanofibers for Cr(VI), As(V) and phenol sorption were 269.2, 258.5 and 152.3 mg g-1, respectively under ZIF-8 concentration of 3 wt.%, adsorbent dosage of 0.5 g/L, pH of 3, equilibrium times of 30 min, and temperature of 25 °C. The kinetic, and isotherm parameters of adsorption process using chitosan-g-PNVCL/ZIF-8 nanofibers were evaluated. In ternary system, the central composite design was applied to investigate the interaction influence of initial concentrations of selected metal ions and phenol on the performance of nanofibrous adsorbent. The obtained results showed the high capability of nanofibers for the removal of heavy metals and organic pollutants from water.

Doxorubicin hydrochloride - Loaded electrospun chitosan/cobalt ferrite/titanium oxide nanofibers for hyperthermic tumor cell treatment and controlled drug release.

In the present study, the potential of doxorubicin hydrochloride (DOX)-loaded electrospun chitosan/cobalt ferrite/titanium oxide nanofibers was studied to investigate the simultaneous effect of hyperthermia and chemotherapy against melanoma cancer B16F10 cell lines. The cobalt ferrite nanoparticles were synthesized via microwave heating method. The titanium oxide nanoparticles were mixed with cobalt ferrite to control the temperature rise. The synthesized nanoparticles and nanofibers were characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and vibrating sample magnetometer (VSM) analysis. The DOX loading efficiency and in vitro drug release of DOX from nanofibers were investigated at both physiological and acidic conditions by an alternating of magnetic field and without magnetic field effect. The fastest release of DOX from prepared magnetic nanofibers was observed at acidic pH by alternating of magnetic field. The antitumor activity of synthesized nanofibers was also investigated on the melanoma cancer B16F10 cell lines. The obtained results revealed that the DOX loaded-electrospun chitosan/cobalt ferrite/titanium oxide nanofibers can be used for localized cancer therapy.