Meta-Analysis of Efficacy and Safety of Prone Enteral Nutrition in Critically Ill Ventilated Patients.

Objective: Assessing the safety and efficacy of enteral nutrition in critically ill patients receiving prone position ventilation is essential to optimize treatment strategies for critically ill patients. Systematically evaluate the effectiveness and safety of prone position enteral nutrition in critically ill ventilated patients, providing a reference for clinical decision-making. Methods: We conducted a comprehensive search for relevant studies on the safety and efficacy of enteral nutrition in prone ventilation patients. Our search encompassed randomized controlled trials, quasi-experimental studies, and cohort studies, utilizing databases including PubMed, Embase, and Scopus. The search duration spanned from May 2000 to May 2023. Inclusion and exclusion criteria were applied to select eligible literature, followed by data extraction and quality assessment. We employed specific keywords and filters in our search strategy to ensure a robust selection of studies. Subsequently, statistical analysis was performed utilizing RevMan 5.2 software to synthesize and interpret the findings effectively. Result: Five articles were ultimately included, with a total of 372 patients undergoing prone ventilation. The meta-analysis results showed that patients receiving enteral nutrition during prone and supine ventilation had higher levels of gastric residue incidence [RR = -0.01, 95% CI: (-0.08, 0.06), P = .77]. There was no significant difference in the incidence of vomiting/reflux between the prone position group and the control group [RR = 0.60, 95%CI: (0.15-2.45), P = .48]. Prone position ventilation had no significant effect on the incidence of ventilator-associated pneumonia (VAP) [RR = 1.00, 95%CI: (0.14-6.90), P = 1.00]. There was no significant difference in the rate of enteral nutrition interruption between the prone position group and the control group [RR = 0.65, 95%CI: (0.28-1.52), P = .32]. Conclusion: Enteral nutrition in critically ill patients receiving prone position ventilation was not associated with high levels of gastric residual, vomiting or reflux, ventilator-associated pneumonia, or increased incidence of enteral nutrition interruption.

A comparative study for accessing primary healthcare between planning assessment and actual utilization for older adults: a case from Dalian City, China.

Introduction: As China has rapidly evolved into an aging society, the Chinese government has developed a community-oriented primary healthcare system to vigorously expedite the transfer of primary health care (PHC) from higher-level hospitals to community health centers (CHCs). However, current planning standards for CHCs have not considered the heterogeneity of older adults in supply-demand services, such that the areas with severe aging may comprise of underestimated levels of accessibility. Methods: This study focuses on the gap in PHC access between planning assessment and actual utilization for older adults. We conducted an empirical study in the city area of Dalian based on the check-in and survey data from CHCs during the COVID-19 pandemic. A comparison model was built to calculate matching probability using a modified Gaussian Two-Step Floating Catchment Area (G2SFCA) method. Results: As indicated by the results, the communities in the primary healthcare shortage area (PHCSA) increased 6.8% by considering the heterogeneity of older adults; these communities with underserved PHC were ignored by the current planning assessment. Based on the comparison of actual and theoretical accessibility for older adults, we found that the average matching probability was about 76.6%, which means approximately a quarter of older adults have been misestimated the accessibility of PHC. Discussion: Further analysis for the older adults with mismatched accessibility showed two causes of the gap, one is the lack of connection between the spatial distribution of facilities and the allocation of service supply, and the other is the subjective cross-catchment visit to CHCs for older adults.

Symptom cluster trajectories and sentinel symptoms during the first cycle of chemotherapy in patients with lung cancer.

PURPOSE: To investigate symptom severity, symptom cluster trajectories and sentinel symptoms during chemotherapy cycle 1 in patients with lung cancer. METHODS: Patients with lung cancer were recruited to complete the MD Anderson Symptom Inventory (MDASI) and First Appearance of Symptoms Time Sheet daily during the first week of chemotherapy cycle 1. Latent class growth analysis was performed to examine symptom cluster trajectories. The Apriori algorithm combined with the time of the first appearance of symptoms after chemotherapy was used to determine the sentinel symptoms of each symptom cluster. RESULTS: A total of 175 lung cancer patients participated in the study. Five symptom clusters were identified: class 1 (difficulty remembering-numbness-hemoptysis-weight loss), class 2 (cough-expectoration-chest tightness-shortness of breath), class 3 (nausea-sleep disturbance-drowsiness-constipation), class 4 (pain-distress-dry mouth-sadness-vomiting), class 5 (fatigue-lack of appetite). Sentinel symptoms were found to be cough (class 2) and fatigue (class 5), while none were found for other symptom clusters. CONCLUSION: The trajectories of five symptom clusters were observed during the first week of chemotherapy cycle 1 and the sentinel symptoms of each cluster were explored. The study has important significance for the effective management of symptoms and the quality of nursing care for patients. At the same time, alleviating sentinel symptoms may reduce the severity of the whole symptom cluster, reducing medical resources and improving quality of life for lung cancer patients.

APPLICATION OF CT PULMONARY ANGIOGRAPHY WITH "ULTRA-DOUBLE-LOW" AND ITERATIVE MODEL RECONSTRUCTION FOR ACUTE PULMONARY EMBOLISM.

The study is to investigate the feasibility of computed tomography pulmonary angiography (CTPA) with iterative model reconstruction (IMR) and "Ultra-double-low" (Ultra-low dose, Ultra-low contrast agent volume). Thirty-six patients who tested positive for pulmonary embolism in CTPA were enrolled in the study. Another CTPA was performed 1 week after thrombolytic therapy. The first examination was routine CTPA (Routine Group) with the parameters as follows: automatic mA scanning, 120 kV and image reconstruction by using iDose4 iterative reconstruction (Lever 4), iodine concentration and dose of contrast agent: 300 mgI/ml and 0.5 gI/kg, respectively. The latter one was ultra-low dose CTPA examination (Ultra-low Group): 40 mAs, 80 kV and IMR (Lever 3), contrast agent: 300 mgI/ml and 15 mL, respectively. Effective dose (ED), CT dose index volume (CTDIvol), dose length product (DLP), attenuation of pulmonary artery, contrast noise ratio (CNR) and signal noise ratio (SNR) were recorded and calculated. The imaging qualities were subjectively assessed. The Eds/CTDIvols/DLPs of Ultra-low Group are lower than the Routine Group (P < 0.05). The differences in attenuation between the two groups are not significant (P > 0.05). The differences in CNR and SNR between the two groups are significant (P < 0.05). The differences in imaging qualities between the two groups when subjectively assessed are not significant (P > 0.05). The 256-slice spiral CT combined with IMR and "Ultra-double-low" is feasible for the acute pulmonary embolism examination and the radiation dose and the volume of contrast agent can be greatly reduced.

Corrigendum: A comparative study for accessing primary healthcare between planning assessment and actual utilization for older adults: a case from Dalian City, China.

[This corrects the article DOI: 10.3389/fpubh.2023.1207098.].

Guben Tongluo Formula Protects LPS-induced Damage in Lamina Propria B Lymphocytes Through TLR4/MyD88/NF-κB Pathway.

OBJECTIVE: The main pathological feature of immunoglobulin A nephropathy (IgAN), an autoimmune kidney disease, is the deposition of IgA immune complexes, accompanied by mesangial cell proliferation and elevated urine protein. The Guben Tongluo formula (GTF) is a traditional Chinese medicine prescription, which has predominant protective effects on IgAN. However, the therapeutic mechanism of the GTF in IgAN remains elusive. The present study aimed to determine the effects of GTF in treating IgAN via regulating the TLR4/MyD88/NF-κB pathway. METHODS: In the present study, lamina propria B lymphocytes were treated with different concentrations of lipopolysaccharide (LPS) (0, 1, 5, 10 and 20 ng/mL). Flow cytometry was used to define positive CD86+CD19+ cells. CCK-8 assay was used to examine cell proliferation. RNAi was used to induce TLR4 silencing. qRT-PCR and Western blotting were used to determine gene expression. RESULTS: It was found that the LPS dose-dependently increased the content of IgA and galactose-deficient IgA1 (Gd-IgA), the levels of TLR4, Cosmc, MyD88 and phosphorylated (p)-NF-κB, and the ratio of CD86+CD19+ and IgA-producing B cells. However, the TLR4 knockdown reversed the role of LPS. This suggests that TLR4 mediates the effects of LPS on lamina propria B lymphocytes. Furthermore, the GTF could dose-dependently counteract the effects of LPS and TLR4 overexpression on lamina propria B lymphocytes through the TLR4/MyD88/NF-κB pathway. CONCLUSION: Collectively, these results demonstrate that the GTF can regulate the TLR4/MyD88/NF-κB pathway to treat IgAN model lamina propria B lymphocytes stimulated by LPS.

TRIM40 inhibits IgA1-induced proliferation of glomerular mesangial cells by inactivating NLRP3 inflammasome through ubiquitination.

IgA nephropathy, as the most common type of glomerulonephritis, causes chronic renal disease and progresses into kidney failure. Aberrant IgA deposition in the glomerular mesangium induces NLRP3 inflammasome activation for massive local inflammation, and is recognized as the primary pathogenesis in IgA nephropathy. Tripartite motif (TRIM)-containing proteins are E3 ubiquitin ligases that possess crucial regulatory functions in innate immunity, but their functional roles in IgA nephropathy are still unclear. Here, we aimed to identify TRIM-containing proteins that regulate IgA nephropathy and their underlying mechanisms. An in vitro IgA1-induction model was established in glomerular mesangial cells (GMCs) and showed that IgA1 could promote GMC proliferation by activating NLRP3 inflammasome. TRIM40, which was downregulated by IgA1 and interacted with NLRP3, was recognized as a promising candidate. In addition, TRIM40 could suppress IgA1-induced GMC proliferation by inhibiting the activation of NLRP3 inflammasome. Based on coimmunoprecipitation and ubiquitination assays, we confirmed that TRIM40 could mediate the ubiquitination of NLRP3, which explained its regulatory effects on NLRP3 inflammasome and GMC proliferation. More importantly, a dominant-negative mutant of TRIM40 lacking the RING domain (ΔRING) did not affect NLRP3 ubiquitination, and had no effects on IgA1-induced GMC proliferation or NLRP3 inflammasome activation. This study revealed the biological functions of TRIM40 in IgA nephropathy, facilitating its application as therapeutic target for IgA nephropathy and other NLRP3 inflammasome-relevant diseases.

Radiomics Based on Multimodal MRI for the Differential Diagnosis of Benign and Malignant Breast Lesions.

BACKGROUND: MRI-based radiomics has been used to diagnose breast lesions; however, little research combining quantitative pharmacokinetic parameters of dynamic contrast-enhanced MRI (DCE-MRI) and diffusion kurtosis imaging (DKI) exists. PURPOSE: To develop and validate a multimodal MRI-based radiomics model for the differential diagnosis of benign and malignant breast lesions and analyze the discriminative abilities of different MR sequences. STUDY TYPE: Retrospective. POPULATION: In all, 207 female patients with 207 histopathology-confirmed breast lesions (95 benign and 112 malignant) were included in the study. Then 159 patients were assigned to the training group, and 48 patients comprised the validation group. FIELD STRENGTH/SEQUENCE: T2 -weighted (T2 W), T1 -weighted (T1 W), diffusion-weighted MR imaging (b-values = 0, 500, 800, and 2000 seconds/mm2 ) and quantitative DCE-MRI were performed on a 3.0T MR scanner. ASSESSMENT: Radiomics features were extracted from T2 WI, T1 WI, DKI, apparent diffusion coefficient (ADC) maps, and DCE pharmacokinetic parameter maps in the training set. Models based on each sequence or combinations of sequences were built using a support vector machine (SVM) classifier and used to differentiate benign and malignant breast lesions in the validation set. STATISTICAL TESTS: Optimal feature selection was performed by Spearman's rank correlation coefficients and the least absolute shrinkage and selection operator algorithm (LASSO). Receiver operating characteristic (ROC) curves were used to assess the diagnostic performance of the radiomics models in the validation set. RESULTS: The area under the ROC curve (AUC) of the optimal radiomics model, including T2 WI, DKI, and quantitative DCE-MRI parameter maps was 0.921, with an accuracy of 0.833. The AUCs of the models based on T1 WI, T2 WI, ADC map, DKI, and DCE pharmacokinetic parameter maps were 0.730, 0.791, 0.770, 0.788, and 0.836, respectively. DATA CONCLUSION: The model based on radiomics features from T2 WI, DKI, and quantitative DCE pharmacokinetic parameter maps has a high discriminatory ability for benign and malignant breast lesions. LEVEL OF EVIDENCE: 3 TECHNICAL EFFICACY STAGE: 2 J. Magn. Reson. Imaging 2020;52:596-607.

Histogram analysis of diffusion kurtosis imaging in the differentiation of malignant from benign breast lesions.

OBJECTIVE: To assess the diagnostic accuracy of histogram analysis of diffusion kurtosis imaging (DKI) in breast lesions. MATERIALS AND METHODS: Our institutional review board approved this retrospective study. Seventy-two breast lesions (30 benign and 42 malignant) in 71 patients were histopathologically confirmed. All breast lesions were evaluated by 3.0-T diffusion-weighted imaging (DWI) with 4 b-values of 0, 500, 800, and 2000s/mm2 and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Histogram analyses of conventional DWI and DKI were performed using FireVoxel software for whole lesions. The parameters included apparent diffusion coefficient (ADC), diffusivity (D), and kurtosis (K). The metrics of ADC and DKI parameters (D and K) for benign lesions were compared with those for malignant lesions. The effectiveness of the ADC and DKI parameters (D and K) for diagnosing breast lesions was analysed using receiver operating characteristic (ROC) regression models. RESULTS: For the malignant breast lesions, the mean, median, and 10th/25th/75th percentile values of ADC and D were significantly lower, while the skewness of ADC and D were significantly higher in comparison of the benign lesions(all p < 0.05). The malignant lesions had significantly higher mean, median, and 10th/25th/75th/90th percentile K values than did the benign lesions (all p < 0.05). Within each set of parameters, the 10th percentile ADC (Az = 0.752) and D, (Az = 0.834) coupled with the 75th percentile K (Az = 0.904) were the best metrics for differentiating benign from malignant breast lesions. After comparing the parameters in pairs, the Az for the 75th percentile K was significantly higher than that for the 10th percentile ADC (p = 0.0321) in differentiating benign from malignant breast lesions. When comparing the combination of the 75th percentile K and the 10th percentile D (Az = 0.937) with the 10th percentile D, 75th percentile K and the mean K, a significantly higher Az was observed for the combination than that for the 10th percentile D and the mean K (p = 0.0097 and p = 0.0431, respectively). The diagnostic sensitivity and specificity of the combination of the 75th percentile K and the 10th percentile D were 85.71% and 93.33%, respectively. CONCLUSION: Histogram analysis of DKI can accurately reflect the histologic characteristics and heterogeneity and is a reliable method for diagnosing breast lesions.