Screening of key modules and key genes in the prevention of skin cancer development based on gene volcano plot and WGCNA.

BACKGROUND: Skin cancer, a prevalent form of cancer that is on the rise worldwide, requires proactive prevention strategies to reduce the burden of screening, treatment, and mortality. The KEGG research highlighted the significant involvement of red module genes in protein digestion and absorption. These findings provide valuable insights into the underlying molecular mechanisms associated with skin cancer susceptibility, offering potential targets for further research and development of preventive strategies. MATERIALS AND METHODS: Hub genes numbered 130. "limma" in R found 600 DEGs from GSE66359 dataset. DEGs are enriched in BP: chromosome segregation, CC: chromosomal region, and MF: DNA replication origin binding, according to GO analysis. Cell cycle was enriched in DEGs by KEGG and GSEA. Finally, significant genes were COL5A1, CTHRC1, ECM1, FSTL1, KDELR3, and WIPI1. RESULTS: ECM1 and WIPI1 greatly prevented skin cancer. This study created a coexpression network using WGCNA to investigate skin cancer susceptibility modules and cardiovascular disease genes. CONCLUSION: Our study finds a module and many important genes that are essential building blocks in the etiology of skin cancer, which may help us understand the molecular mechanisms of disease prevention.

Evaluation of ALA-PDT combined with antibiotics for the treatment of skin ulcers with sinus tract formation: A pilot study.

BACKGROUND: Photodynamic therapy (PDT) is considered an important and innovative treatment for healing skin wounds. Skin ulcers with sinus tract formation are rare and difficult to treat in the field of dermatology. Herein, we utilized optical fibers as a light source to penetrate the sinus tract and applied PDT to treat the sinus tract. OBJECTIVES: To evaluate the efficacy and safety of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) and provide insight into the use of ALA-PDT in the treatment of skin ulcers with sinus tracts. METHODS: Seven patients with skin ulcers with sinus tract formation who were previously unsuccessfully treated with systemic antibiotics, debridement and dressing changes were examined. In the present study, these patients also received ALA-PDT treatment (the ulcers were irradiated with a red LED, and the sinus tracts were irradiated with semiconductor laser optical fibers), which was locally administered every 10 days with a total of 1-5 sessions. RESULTS: Six patients enrolled in the study were fully cured after receiving combination therapy with ALA-PDT and antibiotics for three months. The sinus tract of one patient was cured and the ulcer area of this patient was reduced. All patients experienced varying degrees of redness and pain during treatment but no severe discomfort, and all patients were satisfied with the outcome of the treatment. CONCLUSION: Local ALA-PDT combined with antibiotics could be a safe and effective treatment approach for skin ulcers with sinus tract formation.