[The effects of different exercise modes on Rab5 protein and glucose metabolism in skeletal muscle of type 2 diabetic mellitus rats].

Objective: To investigate the effects of continuing exercise and load-bearing interval exercise on skeletal muscle tissue cell morphology, Ras-related proteins 5 (Rab5) mRNA and protein expression and glucose metabolism in skeletal muscle of type 2 diabetic mellitus (T2DM) rats. Methods: Eight SD rats were selected as controls group (CR), the others SD rats were fed with high fat and high sugar diet for 6 weeks before injecting STZ (35 mg/kg) to construct the T2DM model. Twenty-four T2DM rats were randomly devided into T2DM model group (DRM), continuing exercise group (DCRE) and load-bearing interval exercise group (DWRE), 8 rats in each group. DCRE exercise protocol, that was 15 m/min (10 min), 20 m/min (40 min), 15 m/min (10 min), during the first 1～2 weeks, and 18 m/min (10 min), 25 m/min (40 min), 15 m/min (10 min), during the second 3～8 weeks. DWRE exercise protocol: load weight 15% / 1～2 weeks, 30% / 3～4 weeks, 45% / 5～8 weeks, with 15 m/min (5 min), 12 groups and 3 min rest between groups. After 8 weeks, pathological and morphological changes of skeletal muscle were observed by HE. Rab5 and Glucose transporte 4 (GLUT4) mRNA expressions of skeletal muscle were tested by qRT-PCR. Rab5 protein expression in skeletal muscle was tested by immunofluorescence histochemistry and Western blot, and plasma Rab5 and Glycosylated Hemoglobin (GHb) concentrations were detected by ELISA. Results: Comparison with CR, DRM showed pathological damage of skeletal muscle, the expressions of Rab5 mRNA, protein and GLUT4 mRNA were all decreased in skeletal muscle (P＜0.01), the serum levels of Rab5 and GHb were both significantly elevated (P＜0.01). Comparison with DRM, both DCRE and DWRE significantly improved pathological damages of skeletal muscle, the expressions of Rab5 mRNA, protein and GLUT4 mRNA were all increased in skeletal muscle (P＜ 0.05, P＜0.01), the serum levels of Rab5 and GHb were decreased (P＜0.05, P＜0.01), and there was no statistical difference between DCRE and DWRE groups (P＞0.05). Conclusion: Two exercise modes can improve the pathological injury of skeletal muscle in type 2 diabetic rats, and enhance GLUT4 transport capacity by improving the expression of Rab5 gene and protein in skeletal muscle, and alleviate the imbalance of glucose metabolism homeostasis in skeletal muscle. However, there was no significant difference between the effects of two exercise modes on Rab5 protein and glucose metabolism in skeletal muscle.

[Effect of exercise on adiponectin in aged obese rats].

OBJECTIVE: To explore effects of exercise on the expression of adiponectin mRNA and protein in visceral adipose tissue, plasma adiponectin concentration, and insulin resistance of aged obese rats. METHODS: Male SD rats age to 21 days old were fed with high-fat diet (fat percentage was 36.3% to 40.0%) for three stages of adolescence, maturity and old age to establish elderly obesity rats model. When the rats aged to 60 weeks old, natural growing elderly rats were randomly divided into control group (C) and aged exercise group (AE), n=6; elderly obesity rats were randomly divided into obesity control group (OC) and obesity exercise group (OE), n=6. The treadmill grade was 0°, the exercise speed and time were 15 m/min×15 min, 4 groups each time, between consecutive groups the rats had 5 minutes rest, the rats were exercised for 60 minutes every day, five days a week, continuous exercise for 8 weeks. Then, the expressions of adiponectin mRNA and protein in visceral adipose tissue were determined. The concentrations of blood glucose, plasma adiponectin and insulin were measured. Insulin resistance was calculated. RESULTS: Comparison with control group, the expressions of adiponectin mRNA and protein were obviously decreased, the concentration of blood glucose and insulin resistance were significantly increased in obesity control group, while the expressions of adiponectin mRNA and protein were obviously increased. Comparison with obesity control group, the expressions of adiponectin mRNA and protein, the concentration of plasma adiponectin were obviously increased, the concentration of blood glucose and insulin resistance were significantly decreased in obesity exercise group. CONCLUSIONS: Adiponectin mRNA and protein expression in visceral adipose tissue is decreased and accompanied by high blood glucose and insulin resistance in elderly obesity rats. Exercise can increase the adiponectin mRNA and protein expression in visceral adipose tissue, elevate levels of plasma adiponectin, and decrease the level of blood glucose and insulin resistance in elderly obesity rats.

[Effect of different intensity exercise prescription on visceral adipose Vaspin gene, protein expression and plasma Vaspin concentration in aged obese rats].

OBJECTIVE: To investigate effects of different intensity exercise prescription on visceral adipose tissue-derived serine protease inhibitor( Vaspin) mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats. METHODS: SD rats as three stage of fattening divided the growth period, middle-aged and elderly, to establish rats model of elderly obesity, 6 natural growing aged rats were selected as blank control group, 18 elderly obese rats were randomly divided into 3 groups, 6 rats in each group: obese control group, low intensity exercise group, moderate intensity exercise group. Exercise prescription were 12 m /min × 15 min for each group, 4groups / times, rest between groups 5min, 60 min/time. 15 m/min×15 min for each group, 4 groups / times, rest between groups 5min, 60 min / time. Vaspin mRNA expression was measured by qRT-PCR and Vaspin protein expression was measured by WB in visceral adipose tissue and plasma Vaspin concentration was measured by ELISA after exercise. RESULTS: After exercise intervention, (1) Vaspin mRNA(×103) expression:Obese control group(25. 761±11. 581) was higher than the control group(12. 161±5. 648). (P<0. 01), low intensity exercise group and moderate intensity exercise group((10. 482±2. 668), (13. 478±5. 115)) were lower than obese control group (P<0. 01), moderate intensity exercise group was higher than low intensity exercise group, but the difference was not statistically significant (P>0. 05). (2) Vaspin protein expression: Obese control group was higher than the control group (P<0. 01), low intensity exercise group was lower than obese control group (P<0. 05), but moderate intensity exercise group was higher than obese control group (P<0. 01). (3) Plasma Vaspin concentration: Obese control group((25. 000±3. 767) ng/mL) was higher than the control group((23. 765±3. 045) ng/m L), low intensity exercise group and moderate intensity exercise group((25. 637±5. 240) ng/mL and (26. 422±3. 4175) ng/mL) was higher than obese control group, but the difference were not statistically significant( P >0. 05). ( 4) Blood glucose and insulin sensitivity index: Blood glucose of obese control group was higher than the control group( P < 0. 01). Insulin sensitivity index of obese control group was lower than the control group, but the difference was not statistically significant( P > 0. 05). Blood glucose of low and moderate intensity exercise groups were lower than obese control group( P < 0. 05), but insulin sensitivity index of low and moderate intensity exercise groups were higher than obese control group( P < 0. 01). CONCLUSION: Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats are higher. Low intensity exercise can reduce Vaspin mRNA and protein expression, but increase exercise intensity may improve Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats.

[Effects of exercise and resveratrol on retinol binding protein 4, blood glucose and insulin sensitivity in aged obese rats].

OBJECTIVE: To explore the effects of exercise and resveratrol on retinol binding protein 4( RBP4) mRNA and protein expression in visceral adipose tissue, plasma RBP4 concentration and blood glucose and insulin sensitivity of aged obese rats. METHODS: To establish elderly obese SD rat model, 6 aged rats of natural growth were selected normal group, 24 obese rats were randomly divided into four groups: obesity control group, exercise group, resveratrol group, exercise combined with resveratrol group. For Resveratrol treatment, each rat was fed 52. 5 mg/( kg d), 5 times/week, continuous intervention for 8 weeks. After intervention, measured blood glucose, insulin sensitivity, visceral adipose tissue RBP4 mRNA expression( qRT-PCR), protein expression( Western blot) and plasma RBP4 concentration( ELISA). RESULTS: After 8week intervention, ( 1) RBP4 mRNA expression: The obesity group( 2. 63 ± 0. 45) was higher than that of the normal group( 2. 10 ± 0. 15)( P < 0. 05). The resveratrol group( 1. 84 ± 0. 33), exercise group( 1. 91 ± 0. 15), and exercise combined with resveratrol group( 2. 131 ± 0. 111) were lower than that of the obesity group( P < 0. 05, P < 0. 01). ( 2) RBP4 protein expression: The obesity group( 1. 346 ± 0. 025) was higher than that of the normal group( 1. 196 ± 0. 017)( P < 0. 01). The exercise group( 1. 025 ± 0. 006)was lower than that of the obesity group( P < 0. 01), The resveratrol group( 0. 735 ±0. 015) and exercise combined with resveratrol group( 0. 701 ± 0. 018) were lower than that of the exercise group( P < 0. 01). The exercise combined with resveratrol group was lower than that of resveratrol group( P < 0. 05). ( 3) Plasma RBP4 concentration: The obesity group [( 16. 00 ± 1. 54) µg/L]was higher than that of the normal group [( 13. 02± 2. 20) µg/L]( P < 0. 01). The exercise group [( 14. 76 ± 1. 56) µg/L] was lower than that of the obesity group, but the difference was not statistically significant. The resveratrol group [( 13. 59 ± 0. 07) µg/L]and exercise combined with resveratrol group[( 12. 98 ± 1. 69) µg/L] were lower than that of the obesity group( P < 0. 05, P <0. 01). ( 4) Blood glucose: The obesity group [( 17. 93 ± 6. 09) mmol/L]was higher than that of the normal group [( 11. 64 ± 3. 57) mmol/L ]( P < 0. 01). The exercise group [( 13. 36 ± 1. 82) mmol/L]was lower than that of the obesity group( P < 0. 05), the resveratrol group [( 15. 24 ± 2. 19) mmol/L] and exercise combined with resveratrol group [( 13. 95 ± 2. 26) mmol/L] were lower than that of the obesity group, but the difference was not statistically significant( P > 0. 05). ( 5) Insulin sensitivity: The obesity group( 0. 37 ± 0. 02) was lower than that of the normal group( 0. 39 ± 0. 02)( P < 0. 05). The resveratrol group( 0. 38 ± 0. 01) and the exercise group( 0. 39 ± 0. 02)were higher than that of the obesity group, but the difference was not statistically significant, the exercise combined with resveratrol group( 0. 39 ± 0. 15) was higher than that of the obesity group( P < 0. 05). Above indexes( except protein expression) were no significant difference between the each intervene groups( P > 0. 05). CONCLUSION: RBP4 mRNA expression and protein expression in the visceral adipose tissue of aged obese rats, plasma RBP4 concentration and blood glucose were increased, and insulin sensitivity was decreased. Exercise and resveratrol could reduce the expression of RBP4 mRNA and protein expression and plasma RBP4 concentration. The simple exercise could significantly reduce the blood glucose and exercise combined with resveratrol could significantlyimprove insulin sensitivity. The decrease in protein expression, plasma RBP4 concentration and improve insulin sensitivity, exercise combined with resveratrol showed synergy.

[Effects of different intensities exercise combined with resveratrol on RBP4 in aged obese rats].

OBJECTIVE: To investigate the effects of different intensity exercise combined with resveratrol on retinol binding protein 4(RBP4) mRNA and protein expression in visceral adipose tissue and plasma RBP4 concentration of aged obese rats. METHODS: Eighty male SD rats aged 3 weeks were randomly divided into the control group and the experimental group. Rats of the control group(C) were fed with ordinary feed of 6% fat (n=12). The experimental group consisted of 68 rats, which were fed with 36%~40% high fat feed in three stages. The rat model of elderly obesity was established successfully. Twenty-four obese rats were selected and randomly divided into 4 groups, including obese group (CO), resveratrol obese group (RO), low intensity exercise combined with resveratrol group (LRO) and moderate intensity exercise combined with resveratrol group (MRO). There were 6 rats in each group. The exercise intensities of the LRO group and MRO group were (12 m/min×15 min) and (15 m/min×15 min). Every day was exercised for 60 minutes. The rats in RO, LRO or MRO were treated with resveratrol at the dose of 52.5 mg/kg·d, while the rats in the control group were fed with the same amount of purified water, continued intervention for 8 weeks. After 8 weeks, samples of blood, adipose tissue of around the kidney, testicle, vessel and visceral were collected. Blood glucose and plasma RBP4 concentration were measured. Insulin sensitivity(ISI) was calculated. The expressions of RBP4 mRNA and protein were also determined. RESULTS: Compared with normal (C) group, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the model (CO) group were increased obviously (P<0.05, P<0.01). ISI was decreased obviously (P<0.05). Compared with model (CO) group, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the RO and LRO and MRO groups were decreased significantly(P<0.05, P<0.01), while ISI was increased markedly (P<0.05). Compared with the RO and LRO groups, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the MRO group were decreased, while ISI was increased, but there was no significant difference. CONCLUSIONS: Different intensities exercise combined with resveratrol could reduce the RBP4 mRNA and protein expression in visceral adipose tissue and plasma RBP4 concentrations of aged obese rats, but less affected by exercise intensity.

[Effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats].

OBJECTIVE: To exploer effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats. METHODS: SD rats as three stage of fattening divided the growth period, middle-aged and elderly, toestablish rats model of elderly obesity. 6 natural growing aged rats were selected as blank control group( C). 24 elderly obese rats were randomly divided into 4 groups, 6 rats in each group: obese control group( OC), supplementary resveratrol group( OR), exercise group( OE), exercise combined with resveratrol group( OER). Running speed and time of animal were 15 m/min×15 min for each group, 4 groups/times, rest between groups5 min, 60min/time. For resveratrol treatment, each rat was fed 52. 5mg/kg, one time/day, 5 times/week, for 8 weeks. The content of blood glucose was measured, the insulin resistance was calculated, testing the expression of resistin mRNA in visceral adipose tissue and plasma Resistin concentration. RESULTS: Body weight, visceral fat mass, body fat ratio, and blood glucose, and insulin resistance of OC group were higher than C group( P < 0. 01, P < 0. 05), Resistin mRNA×10~3 expression in visceral adipose tissue and plasma concentration of OC group(( 29. 80 ± 4. 91) µg/L and( 4. 30 ± 1. 93) µg/L, repectively) were higher than C group(( 23. 09 ± 6. 12) µg/L and( 3. 49 ± 0. 42) µg/L, repectively), but the difference was not statistically significant. Body weight, visceral fat mass, body fat rate, and blood glucose, and insulin resistance of OE group and OER group were lower than OC group( P < 0. 01, P < 0. 05), of OR group were lower than OC group, of OER group were lower than OE group, but the difference was not statistically significant. Resistin mRNA×10~3 expression in visceral adipose tissue of OR group( 21. 63 ±4. 91) was lower than OC group( P < 0. 05), of OE group and OER group(( 28. 21 ± 4. 80), ( 25. 22 ± 11. 12), respectively) were lower than OC group, but the difference was not statistically significant. Plasma resistin concentration of OR group and OE group and OER group(( 3. 71 ± 1. 01), ( 3. 29 ± 1. 19) and( 2. 99 ± 0. 44) µg/L, respectively), were lower than OC group, of OER group were lower than OE group, of OE group were lower than OR group, but the difference was not statistically significant. CONCLUSION: Elderly obesity result in insulin resistance and high blood glucose, resistin expression in visceral adipose tissueand and plasma resistin concentration is higher. In elderly obese rats, simple exercise and exercise combined with resveratrol can significantly reduce weight, visceral fat mass, body fat ratio, and blood glucose content, and insulin resistance, but that are less affected on resistin expression in visceral fat and plasma resistin concentration. Simple add resveratrol can reduce resistin mRNA expression in visceral adipose tissue.

[Effects of conjugated linoleic acid and exercise on RBP4 of liver and adipose tissues in adolescent obese rats].

OBJECTIVE: To exploer effects of conjugated linoleic acid and exercise on RBP4 of liver and adipose tissues in adolescent obese rats. METHODS: 8 adolescent SD rats were selected as blank control (C) with normal diet. Obese rats were modeled with high fat feeding for 7 weeks. 32 obese rats were selected and randomly divided into 4 groups: control group (OC), CLA group ( OCC), exercise group (OM), exercise combined with CLA group (OMC). Exercise treatment were performed on animal treadmill with running speed of 21 - 25 m/min, 60 min/every time. For CLA treatment, each rat was fed 1.6 g/kg, one time/day, 5 times/week, for 8 weeks. Animals were anesthesia treated after experiment, blood and body tissues were collected. The content of blood glucose was measured, the insulin sensitivity was calculated, testing the expression of RBP4 mRNA in visceral adipose tissue, expression of RBP4 protein in liver cells and plasma RBP4 concentration. RESULT: (1) Weight, weight growth rate and body fat percentage of OM, OMC groups were lower than OC, OCC groups (P < 0.01). (2) Blood glucose content of OM, OMC groups were significantly lower than OC, OCC groups (P < 0.01), while insulin sensitivity of OM, OMC groups was higher than OC, OCC groups (P < 0.01). (3) Expression of RBP4 mRNA of OM, OMC groups in visceral adipose tissue was lower than OC, OCC groups (P < 0.01). Expression of RBP4 protein of OM, OMC groups in liver cells was significantly lower than OC, OCC groups. RBP4 concentration in plasma of OM, OMC groups was lower than OC, OCC groups (P < 0.01). (4) Weight, weight growth rate, insulin sensitivity of OMC group were higher than OM group, but the percentage of body fat, blood glucose content and the expression of RBP4 were lower than OM group, there were no significant differences between them. CONCLUSION: Simple exercise and exercise combined with CLA conld significantly reduced adolescent obese rats weight, weight growth rate, percentage of body fat, blood glucose content and also reduced RBP4 mRNA expression in visceral adipose tissue, RBP4 protein expression in liver tissue, RBP4 level in plasma,but can increase sensitivity to insulin, in a word, the treatment of exercise and exercise combine with CLA is better than the simple complement of CLA to adolescent obese rats.

[Effects of exercise and conjugated linoleic acid on PPARγ in adolescent obese rats].

OBJECTIVE: To explore effects of exercise and conjugated linoleic acid (CLA) on PPARy in adolescent obese male SD rats. METHODS: obese rats were modeled with high fat feeding, 32 obese rats were selected and randomly divided into control group, static + CLA group, exercise-treated group, exercise + CLA treated group. Blood and adipose tissue. were collected after 8 weeks, and blood lipid was measured. PPARγ mRNA gene expression in adipose tissue was tested using qRT-PCR, PPARγ protein expression in adipose tissue by immunohistochemistry, concentration of PPARγ in plasma by ELISA. RESULTS: (1) TC level of static + CLA group and exercise group were lower than the control group (P <0. 05). TG level of the exercise group and exercise group + CLA were lower than the control group, static + CLA group (P <0. 01). LDL-c level of exercise group and exercise + CLA group was higher than the control group (P <0. 05), HDL-c level have no difference in groups. (2) PPAR-γ concentration in plasma in exercise group, exercise + CLA group was higher than the control group and static + CLA group (P <0. 01). Expression of PPARy mRNA in adipose tissue in exercise group, exercise + CLA group was higher than the control group and static + CLA group (P <0. 01). PPARγ concentration in plasma and expression of PPARy mRNA in adipose tissue in static + CLA group were higher than control group but with no statistical significance. (3) The situation of PPARγ protein expression was the same with the expression of PPARγ mRNA. CONCLUSION: 8 weeks of different intervention methods can reduce the concentration of TG and TC in blood lipid index in adolescent obese rats, TG of exercise and exercise + CLA is better decreasing than simply add CLA. Exercise and exercise + CLA can improve the expression of PPARy mRNA and protein in adipose tissue and plasma PPARγ concentration in rats.

[Effects of different exercise intensity on PPARγ and relative index in adolescent obesity rats].

OBJECTIVE: Discuss the effects of low, medium, high intensity exercise induced by PPAR gamma and related indexes of diet in obsess SD rats. METHODS: Male SD rats are selected to establish experimental obesity model of SD rats, selecting 32 rats of model successfully and randomly divided into control group, low intensity group, moderate intensity group and high strength group. Each exercise group is trained by treadmill training for 8 weeks. After 8 weeks, their blood and adipose tissue are collected and the body fat and blood lipid are measured. The qRT-PCR method is used to measure the expression of PPAR gamma mRNA in adipose tissue, and immunohistochemical staining method are used to measure the protein expression of PPAR gamma, and ELIASA is used to determine the plasma concentrations of PPARgamma. RESULTS: After the exercise, the exponent of rats' weight and body fat have reduced (P <0. 01), and the exponent groups of lee' s index are indiscrimination(P > 0. 05) , TC expressed as moderate intensity and high intensity decreased compared with the control group (P <0. 05). TG levels in low, medium and high intensity group are lower than those in the control group (P. < 0.01), LDL levels in medium and high intensity groups are higher than the control group (P < 0.05).The concentrations of PPAR plasma in low, medium and high intensity group are higher than in the control group (P <0.05). PPARy mRNA expression in adipose tissue of low, medium and high intensity group are enhanced than the control group. Immunohistochemical results of PPARy is in consistent with the expression of PPARgamma CONCLUSION: Method of different intensity of exercise for 8 weeks have play a significant effect on weight loss and body fat is significantly lower. Besides, it can also improve blood lipid effectively and significantly increases the expression of PPARy in plasma and adipose tissue.

[Effects of different intensity exercise on blood glucose, adolescent obesity rats insulin sensitivity and RBP4].

OBJECTIVE: To discuss the effects of different intensity exercise on blood glucose, adolescent obesity rats insulin sensitivity and RBP4. METHODS: The model of rats after 7 weeks, 32 rabbits of obesity prone rats were randomly divided into 4 groups, 8 rats in each group, respectively, control group, low intensity exercise group, moderate intensity exercise group, high intensity exercise group. High strength low in animal treadmill running speed respectively for 15 - 18, 21 - 25 and 28 - 32 m/min, 5 times/week, 60 min/times, for 8 consecutive weeks. Anesthesia for blood and tissue collection after exercise intervention, blood glucose, blood insulin, blood RBP4 concentration, liver RBP4 protein expression and visceral adipose tissue RBP4 mRNA expression. RESULT: The 8 week exercise intervention, low, high strength, in the sugar group was significantly lower than the control group (P < 0.01). Insulin sensitivity was higher than that of the control group (P < 0.01), insulin was lower than the control group difference. Expression of visceral adipose tissue RBP4 mRNA was low, medium and high strength group was lower than the control group, the expression was significantly down regulated (P < 0.01), in the high intensity group, low intensity group lowered significantly (P < 0.01). RBP4 higher than exercise group as control group in the expression of liver tissue protein, in the high intensity group, less the amount of expression. Plasma RBP4 concentration of high strength group was significantly lower than that of the control group (P < 0.01), high strength, low intensity group were lower than those in group (P < 0.01). CONCLUSION: Through the different intensity exercise intervention can reduce blood glucose in obese rats, the plasma RBP4 concentration, improve insulin sensitivity, lower expression of RBP4 mRNA in adipose tissues, liver protein expression decreased, with the exercise intensity increased significantly.

[Effects of the body fat mass and blood sugar and plasma resistin to slim exercise prescription for overweight and obesity students].

OBJECTIVE: To explore the influences of slim exercise prescription on body fat mass, blood sugar and plasma resistin for overweight and obesity students. METHODS: Subjects were 9 males and 13 females for simple overweight and obesity students of freshman and junior. The function capacity (FC) were defined after examine of body shape, physical function and exercise capacity. The slim goals and exercise projects were determined according to different objects. The exercise intensity was 60%-70% of FC and 13-15 levels of RPE. Exercise with each time was 60 min, exercise frequency was 5 times perweek, energy metabolism was 500-600 kcal at a time. The relative indexes were detected after 8 weeks. RESULTS: Implementing programmes of slim exercise prescription for 8 weeks, before and after the experiment in the males and females group. The weight, BMI, percentage of body fat (FAT%), waist and hip circumference ratio (WHR), body surface area (BS), fat indexes, the density of body for overweight and obesity the male and female students were significantly decreased (P < 0.01). Body fat mass (FM) and blood sugar were significantly decreased (P < 0.01). Plasma resistin of the male students were significant different (P < 0.01), but the female students were significant different (P < 0.05). Analysis of Bivariate Correlation was Pearson Correlation, plasma resistin and BMI, WHR the male students had correlation, but the female had no correlation. CONCLUSION: The exercise prescription was safe and sure, and could improve weight, BMI, FAT%, FM, WHR, BS, fat indexes, the density of body, blood sugar, plasma resistin in obesity without the diet control.