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1.
IEEE Trans Biomed Circuits Syst ; 16(6): 1030-1043, 2022 12.
Article in English | MEDLINE | ID: mdl-36191107

ABSTRACT

This work reports the first CMOS molecular electronics chip. It is configured as a biosensor, where the primary sensing element is a single molecule "molecular wire" consisting of a ∼100 GΩ, 25 nm long alpha-helical peptide integrated into a current monitoring circuit. The engineered peptide contains a central conjugation site for attachment of various probe molecules, such as DNA, proteins, enzymes, or antibodies, which program the biosensor to detect interactions with a specific target molecule. The current through the molecular wire under a dc applied voltage is monitored with millisecond temporal resolution. The detected signals are millisecond-scale, picoampere current pulses generated by each transient probe-target molecular interaction. Implemented in a 0.18 µm CMOS technology, 16k sensors are arrayed with a 20 µm pitch and read out at a 1 kHz frame rate. The resulting biosensor chip provides direct, real-time observation of the single-molecule interaction kinetics, unlike classical biosensors that measure ensemble averages of such events. This molecular electronics chip provides a platform for putting molecular biosensing "on-chip" to bring the power of semiconductor chips to diverse applications in biological research, diagnostics, sequencing, proteomics, drug discovery, and environmental monitoring.


Subject(s)
Biosensing Techniques , Electronics , Oligonucleotide Array Sequence Analysis , Semiconductors , DNA/chemistry , Nanotechnology , Biosensing Techniques/methods
2.
J Bacteriol ; 194(10): 2767-8, 2012 May.
Article in English | MEDLINE | ID: mdl-22535943

ABSTRACT

Azospira suillum strain PS (formally Dechlorosoma suillum strain PS) is a metabolically versatile betaproteobacterium first identified for its ability to grow by dissimilatory reduction of perchlorate and chlorate [denoted (per)chlorate]. Together with Dechloromonas species, these two genera represent the dominant (per)chlorate-reducing bacteria in mesophilic freshwater environments. In addition to (per)chlorate reduction, A. suillum is capable of the anaerobic oxidation of humic substances and is the first anaerobic nitrate-dependent Fe(II) oxidizer outside the Diaphorobacter and Acidovorax genera for which there is a completed genome sequence.


Subject(s)
Betaproteobacteria/genetics , Genome, Bacterial , Perchlorates/metabolism , Anaerobiosis , Betaproteobacteria/classification , Betaproteobacteria/metabolism , Molecular Sequence Data
3.
J Bacteriol ; 194(9): 2400-1, 2012 May.
Article in English | MEDLINE | ID: mdl-22493205

ABSTRACT

Pseudogulbenkiania ferrooxidans strain 2002 was isolated as a lithoautotrophic, Fe(II)-oxidizing, nitrate-reducing bacterium. Phylogenetically, it is in a clade within the family Neisseriaceae in the order Nessieriales of the class Betaproteobacteria. It is anticipated that comparative genomic analysis of this strain with other nitrate-dependent, Fe(II)-oxidizing bacteria will aid in the elucidation of the genetics and biochemistry underlying this critically important geochemical metabolism.


Subject(s)
Genome, Bacterial , Neisseriaceae/genetics , Neisseriaceae/metabolism , Nitrates/metabolism , Anaerobiosis , Gene Expression Regulation, Bacterial/physiology , Molecular Sequence Data , Neisseriaceae/classification
4.
J Bacteriol ; 192(15): 4078-9, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20525829

ABSTRACT

"Thermincola potens" strain JR is one of the first Gram-positive dissimilatory metal-reducing bacteria (DMRB) for which there is a complete genome sequence. Consistent with the physiology of this organism, preliminary annotation revealed an abundance of multiheme c-type cytochromes that are putatively associated with the periplasm and cell surface in a Gram-positive bacterium. Here we report the complete genome sequence of strain JR.


Subject(s)
Genome, Bacterial , Gram-Positive Bacteria/genetics , Gram-Positive Bacteria/metabolism , Bioelectric Energy Sources , Molecular Sequence Data
5.
J Bacteriol ; 192(5): 1475-6, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20023012

ABSTRACT

Acidovorax ebreus strain TPSY is the first anaerobic nitrate-dependent Fe(II) oxidizer for which there is a completed genome sequence. Preliminary protein annotation revealed an organism optimized for survival in a complex environmental system. Here, we briefly report the completed and annotated genome sequence of strain TPSY.


Subject(s)
Comamonadaceae/genetics , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Genome, Bacterial , Sequence Analysis, DNA , Anaerobiosis , Iron/metabolism , Molecular Sequence Data , Nitrates/metabolism , Oxidation-Reduction
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