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1.
PMC Biophys ; 2(1): 7, 2009 Aug 24.
Article in English | MEDLINE | ID: mdl-19703298

ABSTRACT

Exposure of human erythrocytes to elevated intracellular calcium causes fragments of the cell membrane to be shed as microvesicles. This study tested the hypothesis that microvesicle release depends on microscopic membrane physical properties such as lipid order, fluidity, and composition. Membrane properties were manipulated by varying the experimental temperature, membrane cholesterol content, and the activity of the trans-membrane phospholipid transporter, scramblase. Microvesicle release was enhanced by increasing the experimental temperature. Reduction in membrane cholesterol content by treatment with methyl-beta-cyclodextrin also facilitated vesicle shedding. Inhibition of scramblase with R5421 impaired vesicle release. These data were interpreted in the context of membrane characteristics assessed previously by fluorescence spectroscopy with environment-sensitive probes such as laurdan, diphenylhexatriene, and merocyanine 540. The observations supported the following conclusions: 1) calcium-induced microvesicle shedding in erythrocytes relates more to membrane properties detected by diphenylhexatriene than by the other probes; 2) loss of trans-membrane phospholipid asymmetry is required for microvesicle release.PACS Codes: 87.16.dj, 87.16.dt.

2.
Biophys J ; 96(7): 2709-18, 2009 Apr 08.
Article in English | MEDLINE | ID: mdl-19348753

ABSTRACT

During apoptosis, physical changes in the plasma membrane prepare the cell for clearance by phagocytes and hydrolysis by secretory phospholipase A(2) (sPLA(2)). The relationships among these changes have not been adequately established, especially for hormone-stimulated apoptosis. This study addresses these issues for glucocorticoid-induced apoptosis in S49 lymphoma cells. Flow cytometry, microscopy, and fluorescence spectroscopy were used to assess merocyanine 540 emission, laurdan generalized polarization, phosphatidylserine exposure, caspase activation, and membrane permeability to propidium iodide in the absence and presence of sPLA(2). The earliest event observed was activation of cellular caspases. Results with membrane probes suggest that interlipid spacing also increases early during apoptosis and precedes transbilayer migration of phosphatidylserine, DNA fragmentation, and a general increase in lipid order associated with blebbing and dissolution of the cells. The activity of sPLA(2) appeared to be linked more to lipid spacing than to loss of membrane asymmetry. The early nature of some of these events and their ability to promote activity of a proinflammatory enzyme suggests the possibility of an inflammatory response during T-lymphocyte apoptosis.


Subject(s)
Apoptosis/drug effects , Cell Membrane/drug effects , Cell Membrane/metabolism , Glucocorticoids/pharmacology , Lymphoma/pathology , Animals , Cell Line, Tumor , Cell Survival/drug effects , Enzymes/metabolism , Flow Cytometry , Fluorescent Dyes/metabolism , Hydrolysis , Lipid Metabolism/drug effects , Lymphoma/metabolism , Microscopy , Phosphatidylserines/metabolism , Phospholipases A2/metabolism , Pyrimidinones/metabolism , Spectrometry, Fluorescence , Time Factors , Water/metabolism
3.
Biophys J ; 93(7): 2350-62, 2007 Oct 01.
Article in English | MEDLINE | ID: mdl-17545239

ABSTRACT

During apoptosis, changes occur in lymphocyte membranes that render them susceptible to hydrolysis by secretory phospholipase A(2) (sPLA(2)). To study the relevant mechanisms, a simplified model of apoptosis using a calcium ionophore was applied. Kinetic and flow cytometry experiments provided key observations regarding ionophore treatment: the initial rate of hydrolysis was elevated at all enzyme concentrations, the total amount of reaction product was increased fourfold, and adsorption of the enzyme to the membrane surface was unaltered. Analysis of these results suggested that susceptibility during calcium-induced apoptosis is limited by availability of substrate rather than adsorption of enzyme. Fluorescence experiments identified three membrane alterations during apoptosis that might affect substrate access to the sPLA(2) active site. First, intercalation of merocyanine 540 into the membrane was improved, suggesting an increase in lipid spacing. Second, laurdan detected increased solvation of the lower headgroup region of the membrane. Third, the rate at which fluorescent lipids could be removed from the membrane by albumin was enhanced, implying greater vertical mobility of phospholipids. Thus, it is proposed that the membranes of apoptotic cells become susceptible to sPLA(2) through a reduction in lipid-neighbor interactions that facilitates migration of phospholipids into the enzyme active site.


Subject(s)
Apoptosis , Biophysics/methods , Ionophores/pharmacology , Phospholipases A/chemistry , Animals , Binding Sites , Cell Line, Tumor , Cell Membrane/metabolism , Cell Nucleus/metabolism , Flow Cytometry , Group II Phospholipases A2 , Hydrolysis , Kinetics , Mice , Models, Chemical , Phospholipases A2 , Pyrimidinones/pharmacology
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