Induction of relational schemas: common processes in reasoning and complex learning.

Five experiments were performed to test whether participants induced a coherent representation of the structure of a task, called a relational schema, from specific instances. Properties of a relational schema include: An explicit symbol for a relation, a binding that preserves the truth of a relation, potential for higher-order relations, omnidirectional access, potential for transfer between isomorphs, and ability to predict unseen items in isomorphic problems. However relational schemas are not necessarily coded in abstract form. Predictions from relational schema theory were contrasted with predictions from configural learning and other nonstructural theories in five experiments in which participants were taught a structure comprised of a set of initial-state,operator-->end-state instances. The initial-state,operator pairs were presented and participants had to predict the correct end-state. Induction of a relational schema was achieved efficiently by adult participants as indicated by ability to predict items of a new isomorphic problem. The relational schemas induced showed the omnidirectional access property, there was efficient transfer to isomorphs, and structural coherence had a powerful effect on learning. The "learning to learn" effect traditionally associated with the learning set literature was observed, and the long-standing enigma of learning set acquisition is explained by a model composed of relational schema induction and structure mapping. Performance was better after reversal of operators than after shift to an alternate structure, even though the latter entailed more overlap with previously learned tasks in terms of the number of configural associations that were preserved. An explanation for the reversal shift phenomenon in terms of induction and mapping of a relational schema is proposed. The five experiments provided evidence supporting predictions from relational schema theory, and no evidence was found for configural or nonstructural learning theories.

Individual differences in story comprehension and recall of poor readers.

This study sought to identify poor readers and characterise weaknesses in their knowledge and use of story structure in comprehension and recall. Eighty year 3 children, 20 good readers and 60 poor readers, were selected from an initial pool of children based on factor analysis of scores from three measures of reading ability. The poor readers were then divided into relatively homogeneous subgroups, using eight additional measures of language-reading comprehension, according to a numerical classification procedure. This procedure helped identify specific weaknesses in their language-reading comprehension. All children listened to three stores and retold the stories under free and probe recall conditions. Story recalls were analysed using the STein and Glenn story grammar. Comparison of recalls between the good readers and each of the subgroups of poor readers showed that poor readers in two subgroups evidenced reduced sensitivity to story structure. The children in these subgroups recalled less of the stories overall, recalled less information from story grammar categories to varying extents, and showed patterns of category recall that differed from those of normal readers. Children in one of the subgroups also displayed poor perception of causal relations across story episode boundaries. These results provide evidence of marked heterogeneity in poor readers' story comprehension and recall. Certain subgroups of poor readers may have qualitatively different problems processing stories, relative to other poor readers, which may require a more concerted approach to instruction in story structure.

Effects of closed-head injury on attentional processes: generality of Sternberg's additive factor method.

The present study examined the generality of Shum, McFarland, Bain, and Humphreys' (1990) findings that closed-head injury (CHI) selectively impairs different processes of attention (operationalized in terms of stages of information processing) depending on the severity of, and the time since, injury. The procedure of Shum et al. was based on Sternberg's additive factor method (AFM), with the mode of information processing involved being a physical-directional matching of visual stimuli. The present study followed a similar procedure except that a name-matching task was used. This task was administered to 16 first-year psychology students and two groups of CHI patients (viz., severe short-term (SS) and severe long-term (SL)) with matched control groups. The results obtained replicated the study by Shum in that the SS group was found to be impaired on the identification and response-selection stages of information processing whereas the SL group was found to be impaired only on the response-selection stage. The present study confirms that the AFM is not limited to one specific mode of information process and strengthens the validity of the conclusions made by Shum et al. regarding the effects of CHI on attention.

Assessment of attention: relationship between psychological testing and information processing approaches.

Examined the relationship between the psychological testing and information processing approaches in assessing attention. Eighty-seven subjects (57 females, 30 males) undertook eight psychological tests of attention and a visual-spatial reaction-time task. Using the cognitive-correlate method (Posner & McLeod, 1982), it was found that three components of attention (viz., visual-motor scanning, sustained selective processing, and visual/auditory spanning) derived from the psychological tests could be significantly predicted by specific, yet different, combinations of six indices of information processing (mean reaction time (RT), mean movement time (MT), feature extraction, identification, response selection, and motor adjustment): (a) mean RT and mean MT were found to be the most important indices for predicting performance on visual-motor scanning; (b) the motor-adjustment stage was found to be the most important index for predicting performance on sustained selective processing; (c) the response-selection stage was found to be the most important index for predicting performance on visual/auditory spanning. These relationships are important for supporting the construct-related validity of the psychological tests of attention and for extending the generality and applicability of the RT task.

Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.

The properties of naiveté and large diversity are considered to be essential starting features for combinatorial antibody libraries that eschew immunization by evolution in vitro. We have prepared large libraries with such properties by using random oligonucleotide synthesis, which has the potential to create approximately 10(20) complementarity-determining regions for antibody heavy chains. When combined with light chains and expressed on phage surfaces, high-affinity antibodies could be selected from 5.0 x 10(7) Escherichia coli transformants. Remarkably, antibodies selected only for binding displayed both general structural features known to be important in nature's own antibodies and specific consensus sequences thought to be critical for interaction with the hapten against which the library was selected. Semisynthetic and ultimately totally synthetic combinatorial libraries when coupled with mutation and selection procedures should replace immunization for generation of reagent, therapeutic, and catalytic antibodies.

Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code.

One serious limitation facing protein engineers is the availability of only 20 'proteinogenic' amino acids encoded by natural messenger RNA. The lack of structural diversity among these amino acids restricts the mechanistic and structural issues that can be addressed by site-directed mutagenesis. Here we describe a new technology for incorporating non-standard amino acids into polypeptides by ribosome-based translation. In this technology, the genetic code is expanded through the creation of a 65th codon-anticodon pair from unnatural nucleoside bases having non-standard hydrogen-bonding patterns. This new codon-anticodon pair efficiently supports translation in vitro to yield peptides containing a non-standard amino acid. The versatility of the ribosome as a synthetic tool offers new possibilities for protein engineering, and compares favourably with another recently described approach in which the genetic code is simply rearranged to recruit stop codons to play a coding role.

Site-specific incorporation of nonnatural residues during in vitro protein biosynthesis with semisynthetic aminoacyl-tRNAs.

A method is presented for the incorporation of nonnatural amino acids into proteins during in vitro cell-free translation. A combination of chemical synthesis and run-off transcription was employed to prepare a semisynthetic, nonhypermodified tRNA(Gly) nonsense suppressor acylated with L-3-[125I]iodotyrosine. The presence of this synthetic tRNA during in vitro translation of mRNA containing a nonsense suppression site (e.g., a UAG termination codon) results in the incorporation of the nonnatural amino acid L-3-iodotyrosine into the polypeptide exclusively at the position corresponding to that site. Incorporation of the nonnatural amino acid L-3-[125I]iodotyrosine into the model polypeptide was assessed by quantitative and unambiguous determination of suppression efficiency, read-through, and site specificity of incorporation. Minor modifications of the method employed in this initial experiment also allow the rapid analysis of unlabeled acylated tRNA analogues. Under optimum conditions, the unlabeled amino acid L-3-iodotyrosine was found to be incorporated with a suppression efficiency of 65%. Other nonnatural residues, including N-methylphenylalanine, D-phenylalanine, and phenyllactic acid, were tested in the assay under these same conditions. Suppression efficiencies for this series ranged from 0 to 72% depending on the structure of the residue incorporated. Several other aspects of this methodology, such as tRNA structure and context effects, are briefly discussed.

Effects of closed-head injury on attentional processes: an information-processing stage analysis.

The present study, based on Sternberg's (1969) additive-factor method, examined attentional processes in terms of four information-processing stages (feature extraction, identification, response selection, and motor adjustment). Four task variables were used to operationally define the stages (signal quality, signal similarity, signal-response compatibility, and foreperiod uncertainty). In two studies, a visuo-spatial reaction-time task was undertaken by a group of university subjects (Experiment 1) and by three groups of closed-head-injured patients (severe short-term, severe long-term, and mild short-term) and their corresponding matched controls (Experiment 2). The results indicated that both patients and normals exhibited a similar mode of linear information processing. In addition, it was found that the severe short-term group was impaired on the response-selection stage and response selection stage; the severe long-term group was impaired only on the response-selection; and no evidence of impairment was found for the mild short-term patients. The implications of these findings with reference to the study of attentional processes in closed-head injured patients and to neuropsychological assessment and rehabilitation are discussed.

A comparison of three methods of hydrolysis for estrogen conjugates.

The efficiencies for estrogen conjugate hydrolysis were compared between enzyme hydrolysis, acid solvolysis and a new method, ammonolysis. Samples included: 1) crystalline 1,3,5(10)-estratriene-3, 17 beta-diol disulfate (estradiol 3,17-disulfate), 2) squirrel monkey urine collected following an intravenous injection of [2,4,6,7-H] 1,3,5(10)-estratriene-3,17 beta-diol (estradiol) and 3) a pool of human pregnancy urine. Ammonolysis demonstrated a significant increase over the other techniques in "free" estrogen yields, specifically, from estradiol 3,17-disulfate.