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2.
J Anim Sci ; 91(9): 4366-73, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23825340

ABSTRACT

Two experiments were conducted to evaluate the effect of feeding corn modified wet distillers grain plus solubles (MWDGS; 48% DM) co-ensiled with chopped whole plant corn (WC) on growth performance, dietary intake, and nutrient digestibility of beef cattle. In Exp. 1, 96 Angus-crossed heifers (2 yr old; 522 ± 49.1 kg BW; 5.3 ± 0.1 BCS) were stratified and blocked according to BW and stratified by BCS in each block in a randomized complete block design (24 pens; 4 heifers/pen; 6 treatment replications). Groups were assigned to 1 of 4 dietary treatments for a 62 d trial. Treatments were 1) corn silage (CS) and soybean meal (CON), 2) MWDGS co-ensiled with chopped whole plant corn (WC; CO-EN), 3) CS mixed with MWDGS at feeding (CS+WDG), and 4) CS mixed with dry distillers grain plus solubles (DDGS) at feeding (CS+DDG). In Exp. 2, 4 crossbred beef steers (initial BW = 278 ± 18 kg) fitted with permanent ruminal cannulas were used in a balanced 4 × 4 Latin square to test the effects of feeding MWDGS co-ensiled with WC on DM intake, ruminal fermentation characteristics, and total tract digestibility. There were four 14-d periods, with 10 d for diet adaptation and 4 d for samples collection. Orthogonal contrasts were used and compared CON vs. diets containing distillers grains (DGD), CO-EN vs. diets where distillers grains were mixed at feeding (MIX), and CS+WDG vs. CS+DDG. In Exp. 1, the CON fed heifers resulted in greater G:F (P = 0.04) compared with those fed DGD. However, ADG (P = 0.03), final BW (P = 0.04), and BW gain (P = 0.03) were greatest for DGD diets compared with CON and greatest (P = 0.04) for CO-EN when compared with MIX. Apart from a slightly greater acetate concentration (P = 0.05), which resulted in a greater acetate to propionate ratio (P = 0.03) for the CON diet compared with DGD, no important differences were observed on intake, diet digestibility, or fermentation characteristics when comparing the CON treatment with DGD or when comparing CS+WDG with CS+DDG. However, the CO-EN diet resulted in decreased DMI (P = 0.05) and consequently decreased OM (P = 0.05), N (P = 0.04), and NDF (P = 0.02) intakes compared with MIX. Data from these studies suggest that MWDGS co-ensiled with WC can successfully replace traditional corn-based diets supplemented with soybean meal and corn silage diets supplemented with distillers grains without compromising digestibility and growth performance in pregnant heifers.


Subject(s)
Cattle/physiology , Digestion/drug effects , Energy Intake/drug effects , Feeding Behavior/drug effects , Glycine max/chemistry , Zea mays/chemistry , Animal Husbandry , Animal Nutritional Physiological Phenomena , Animals , Cattle/growth & development , Diet/veterinary , Dietary Supplements/analysis , Female , Fermentation/drug effects , Rumen/drug effects , Rumen/metabolism , Silage/analysis
3.
J Anim Sci ; 90(10): 3574-83, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22665664

ABSTRACT

Two experiments were conducted to evaluate the effects of feeding corn modified wet distillers grain plus solubles (MWDGS) co-ensiled with direct-cut forage (DC) to beef steers, on feedlot performance and total tract digestibility. In Exp. 1, sixty-four crossbred Angus steers (n = 64; 329 ± 43 kg) were blocked by BW and randomly assigned to 1 of 4 dietary treatments: 1) corn-silage and soybean meal (CON), 2) DC co-ensiled with MWDGS (CO-EN), 3) haylage mixed with MWDGS at feeding (H+WDG), and 4) haylage mixed with dry distillers grains plus solubles at feeding (H+DDG). Steers were harvested when they reached an endpoint of 1.1 cm of 12th-rib back fat. In Exp. 2, four ruminally cannulated beef steers (initial BW = 556 ± 31 kg) were used in a 4 × 4 Latin square to evaluate digestibility of diets used in Exp. 1. Experimental periods were 14 d in length with 10 d for diet adaptation and 4 d for sample collection. Three linear orthogonal contrasts were used to compare effects of 1) CON vs. diets containing distillers grains (DGD), 2) CO-EN vs. diets where distillers grains were mixed at feeding (MIX), and 3) H+WDG vs. H+DDG. In Exp. 1, DMI was less (P < 0.001) for steers fed CON compared with those fed DGD and greatest (P < 0.001) for steers fed CO-EN compared with MIX. Corn-silage- and soybean-meal-fed steers had more days on feed than those fed DGD (P = 0.001). Body weight gain (P = 0.02) was greater for CO-EN compared with MIX. Fat thickness at the 12th and 13th ribs was greater (P = 0.03) for DGD steers compared with CON steers. Hot carcass weight was greater (P = 0.03) for steers fed CO-EN compared with those fed MIX. Steers fed CON resulted in greater marbling scores (P = 0.01) compared DGD-fed steers. Longissimus muscle area was greater (P = 0.01) for CON-fed steers compared with all other treatments. In Exp. 2, no differences (P > 0.10) in DM, OM, or N intakes were observed. Apparent total tract DM (P = 0.01) and N (P = 0.02) digestibility were greatest in steers fed CON compared with all others. Steers fed DGD had less (P = 0.02) rumen NH(3) concentrations compared with CON. The CON diet resulted in greater molar concentrations of VFA (P = 0.03) and decreased acetate to propionate ratio (P = 0.03) compared with all DGD. These data suggest that feeding MWDGS co-ensiled with direct-cut forage results in reduced diet digestibility but equal or greater feedlot performance and similar carcass and characteristics when compared with a corn diet supplemented with soybean meal and diets where MWDGS and DDGS were added at the mixer.


Subject(s)
Cattle/physiology , Diet/veterinary , Dietary Supplements , Digestion , Silage , Animals , Cattle/growth & development , Eating , Male , Random Allocation , Zea mays
5.
N Z Vet J ; 56(5): 202-9, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18836499

ABSTRACT

AIM: To characterise the prevalence and distribution of radiographic changes in the hocks and stifles of Thoroughbred yearling colts and fillies in New Zealand and compare them with other populations of young horses. METHODS: Repository radiographs taken in New Zealand for the 2003-2006 Thoroughbred national yearling sales were evaluated by two individual readers. The distribution of radiographic changes was classified as left side only, right side only, or bilateral. Lesions were categorised by type, location, and sex of the yearling. Complete sets of hock and stifle radiographs of 1,505 yearlings were evaluated. RESULTS: Osteophytes or enthesophytes were seen radiographically in the distal tarsal joints of 460/1,505 (31%) horses. Osteochondrosis was seen in the tibiotarsal joint of 66/1,505 (4%) horses, and in the femoropatellar joint of 40/1,505 (3%) horses. Radiographic lucency in the distal or axial aspect of the medial femoral condyle was seen in 247/1,505 (16%) horses, and lucencies consistent with subchondral cyst-like lesions were seen in 26/1,505 (2%) horses. No significant difference was seen in the proportion of colts and fillies with radiographic changes in the hock or stifle. The prevalence of osteochondrosis and subchondral cyst-like lesions in the stifles of the yearlings examined were similar to those reported in Thoroughbred yearling sale horses in the United States of America (USA). The prevalence of changes in the distal tarsal joints was similar to those reported in Standardbred and Thoroughbred yearlings from Scandinavia and the USA. CONCLUSIONS: There was no significant difference in the prevalence of osteochondrosis in the hock and stifle, lucencies in the distal medial femoral condyle, or radiographic changes in the distal tarsal joints between colts and fillies. CLINICAL RELEVANCE: Establishment of the normal prevalence and distribution of radiographic changes in the hocks and stifles of Thoroughbred yearlings in New Zealand will allow comparison with populations of young horses in other countries. Knowledge of the normal prevalence will assist veterinarians to identify abnormally high or low prevalences on individual farms, to further investigate the pathogenesis of the lesions.


Subject(s)
Horse Diseases/diagnostic imaging , Horses , Stifle/diagnostic imaging , Tarsus, Animal/diagnostic imaging , Animals , Animals, Newborn , Commerce , Female , Horse Diseases/diagnosis , Horse Diseases/pathology , Horses/anatomy & histology , Horses/physiology , Male , New Zealand , Prevalence , Radiography , Stifle/pathology , Tarsus, Animal/pathology
6.
J Am Vet Med Assoc ; 224(2): 275-9, 2004 Jan 15.
Article in English | MEDLINE | ID: mdl-14736074

ABSTRACT

OBJECTIVE: To determine the outcome and subsequent fertility of sheep and goats undergoing a cesarean section because of dystocia. DESIGN: Retrospective study. ANIMALS: 85 sheep and 25 goats. PROCEDURE: Medical records were reviewed, and information was obtained on signalment, history, physical examination findings, anesthesia protocol, surgical technique, number of lambs or kids delivered, pre- and postoperative treatments, duration of hospitalization, and postoperative complications. Follow-up information was obtained through telephone conversations with owners. RESULTS: The proportion of sheep admitted to the veterinary teaching hospital during the study period that underwent a cesarean section (4.4%) was significantly higher than the proportion of goats that did (2.2%). Pygmy goats were overrepresented, compared with the hospital population. The most common reason for cesarean section was inadequate dilatation of the cervix. The most common surgical approach was via the left paralumbar fossa. Two hundred one lambs and kids were delivered, of which 116 were dead at delivery or died shortly afterward. Forty-two of the 65 dams with 1 or more dead fetuses had been in stage-2 labor for > 6 hours, and fetal death was significantly associated with a prolonged duration of dystocia. The most common complication following surgery was retained placenta (n = 49). Use of antimicrobials was associated with a lower rate of complications. All 16 dams that were rebred became pregnant and had no problems with dystocia. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that cesarean section is an effective method of resolving dystocia in sheep and goats and does not adversely affect subsequent fertility.


Subject(s)
Cesarean Section/veterinary , Dystocia/veterinary , Fertility , Goats/surgery , Sheep/surgery , Animals , Cesarean Section/methods , Delivery, Obstetric/methods , Delivery, Obstetric/veterinary , Dystocia/surgery , Female , Fetal Death/veterinary , Goats/physiology , Placenta, Retained/epidemiology , Placenta, Retained/veterinary , Postoperative Complications/epidemiology , Postoperative Complications/veterinary , Pregnancy , Pregnancy Outcome , Retrospective Studies , Sheep/physiology
7.
Anat Histol Embryol ; 31(2): 72-7, 2002 Apr.
Article in English | MEDLINE | ID: mdl-12047242

ABSTRACT

Beta-endorphin and substance P were immunolocalized in the articular cartilage, synovial membrane and fibrous joint capsule of dogs. Twelve adult greyhounds were randomly assigned to one of three groups: control, limited exercise, or regimented exercise. On day 0, biopsies of articular cartilage and joint capsule were obtained from the left shoulder joints of dogs receiving limited and regimented exercise. On day 72, biopsies of joint capsule from right and left shoulders and articular cartilage from the right shoulder joint were analysed for the presence of glycosaminoglycans (GAG) and for immunolocalization of substance P and beta-endorphin. Regimented exercise increased the presence of GAGs and immunolocalization of substance P and beta-endorphin in articular cartilage and synovial membrane compared to day 0 biopsies and untreated controls. Localization of beta-endorphin became prominent in and around the chondrocytes. Substance P was increased in chondrocytes and extracellular matrix. Concomitant changes in localization of beta-endorphin and substance P may have a role in the modulation of the microphysiological environment, metabolism, or function of joint tissues in response to low-impact exercise.


Subject(s)
Physical Conditioning, Animal/physiology , Shoulder Joint/chemistry , Substance P/analysis , beta-Endorphin/analysis , Animals , Cartilage, Articular/chemistry , Cartilage, Articular/pathology , Dogs , Female , Immunohistochemistry/veterinary , Male , Random Allocation , Shoulder Joint/pathology , Shoulder Joint/physiology , Synovial Membrane/chemistry , Synovial Membrane/pathology
8.
J Am Vet Med Assoc ; 216(8): 1291-3, 2000 Apr 15.
Article in English | MEDLINE | ID: mdl-10767973

ABSTRACT

OBJECTIVE: To determine clinical and radiographic findings, treatment, and outcome for llamas with long-bone fractures. DESIGN: Retrospective study. ANIMALS: 6 llamas. PROCEDURE: Medical records of llamas admitted between 1993 and 1998 because of long-bone fractures were reviewed. Data collected included age, sex, type of fracture, method of fracture repair, and postoperative complications. The Fisher exact test was used to compare age and sex of the llamas with long-bone fractures with those of the hospital population of llamas. All owners were contacted by telephone to determine perceived postoperative problems and whether the llamas were able to perform as expected. RESULTS: Mean age was 160.8 days (range, 23 to 365 days). There was 1 male and 5 females. Fractures were more likely to occur in young llamas (< or = 1 year old) than in adults. Five of the fractures were attributed to traumatic episodes. Long bones affected included the tibia (n = 2), radius (2), femur (1), and humerus (1). Internal fixation with lag screws, plating, or both was performed on fractures of all llamas except 1; that llama was treated by use of confinement to a stall. None of the llamas had intraoperative complications, but postoperative complications were reported in 2 llamas. All fractures healed eventually, and clients were pleased with outcomes. CONCLUSIONS AND CLINICAL RELEVANCE: Long-bone fractures in llamas are uncommon. Several types of long bone fractures can be successfully repaired by use of internal fixation, resulting in few complications and minimal convalescent time.


Subject(s)
Camelids, New World/injuries , Fractures, Bone/veterinary , Age Factors , Animals , Bone Plates/veterinary , Bone Screws/veterinary , Female , Femoral Fractures/diagnostic imaging , Femoral Fractures/therapy , Femoral Fractures/veterinary , Fracture Fixation, Internal/veterinary , Fracture Healing , Fractures, Bone/diagnostic imaging , Fractures, Bone/therapy , Humeral Fractures/diagnostic imaging , Humeral Fractures/therapy , Humeral Fractures/veterinary , Male , Postoperative Complications/veterinary , Radiography , Radius Fractures/diagnostic imaging , Radius Fractures/therapy , Radius Fractures/veterinary , Records/veterinary , Retrospective Studies , Tibial Fractures/diagnostic imaging , Tibial Fractures/therapy , Tibial Fractures/veterinary , Treatment Outcome
9.
Vet Surg ; 27(6): 555-60, 1998.
Article in English | MEDLINE | ID: mdl-9845219

ABSTRACT

OBJECTIVE: To evaluate the seasonal influence, signalment, type of hay consumed, clinical examination findings, and outcome after surgery for horses with ileal impaction. STUDY DESIGN: A retrospective study. RESULTS: Between 1988 and 1993, 28 horses had surgical correction of ileal impaction. There was a significantly higher rate of ileal impaction during the Fall (September-November, P = .0041 ). Mean duration of clinical signs of abdominal pain before referral was 15 hours. Transrectal palpation was used to localize the impaction in 11 horses. The ileal impaction was reduced by extraluminal massage aided by admixing of intestinal fluid oral to the impaction or injection of fluids intraluminally and then movement of the ingesta into the cecum alone in 24 horses. A total of 26 horses recovered from surgery; 24 horses were discharged from the hospital and eventually returned to previous use. Two horses had fatal postoperative complications: jejunocecostomy dehiscence and development of extensive small intestine adhesions after manual reduction of the impaction. One horse initially treated by manual reduction required jejunocecostomy twice for management of recurrent ileal impaction. Follow-up information was obtained for 21 horses, of which 20 were alive 1 year or longer after surgery. A total of 27 of 28 horses were fed Coastal Bermudagrass hay as the primary type of hay consumed. A total of 9 horses continued to be fed Coastal Bermudagrass hay as the only roughage source, whereas 6 horses were fed Coastal Bermudagrass with at least 50% other hay, and in 6 horses, Coastal Bermudagrass hay was entirely eliminated from the diet. CONCLUSION AND CLINICAL RELEVANCE: Ileal impactions can be successfully reduced by celiotomy and extraluminal massage and injection techniques to soften the ingesta for passage into the cecum without enterotomy or bypass techniques in most horses. Changes in weather and feeding practices in the Fall may account for an increased risk of ileal impaction in horses in the southeastern United States at that time of year.


Subject(s)
Horse Diseases/surgery , Ileal Diseases/veterinary , Intestinal Obstruction/veterinary , Animals , Colic/etiology , Colic/surgery , Colic/veterinary , Female , Horse Diseases/epidemiology , Horses , Ileal Diseases/epidemiology , Ileal Diseases/surgery , Intestinal Obstruction/epidemiology , Intestinal Obstruction/surgery , Male , Retrospective Studies , Risk Factors , Seasons , Southeastern United States/epidemiology , Treatment Outcome
10.
Vet Surg ; 27(4): 331-6, 1998.
Article in English | MEDLINE | ID: mdl-9662775

ABSTRACT

OBJECTIVE: To evaluate a technique for laparoscopic ovariectomy by a ventral abdominal approach in the llama. STUDY DESIGN: Prospective evaluation. ANIMAL POPULATION: Six healthy adult female llamas, aged 2 to 6 years. METHODS: Laparoscopic ovariectomy was performed with the llamas in a 30-degree Trendelenburg position. The mesovarium and proper ligament of the ovary were isolated, and two Hulka clips were placed in opposite directions across each mesovarium and proper ligament of the ovary. Ovaries were removed through either the instrument cannula or instrument portal. RESULTS: The ovaries, mesovarium, and proper ligaments of the ovaries were easily identified using laparoscopy by a ventral abdominal approach. The Hulka Clip Applicator was easy to use, and the Hulka Clips provided secure hemostasis of the vessels within the mesovarium. Mean duration of surgery was 35 minutes (range, 17 to 85 minutes). Two llamas were depressed after surgery but improved within 24 hours. No other complications occurred during an 18-month follow-up period. CONCLUSIONS: Laparoscopic ovariectomy in the llama was facilitated by the use of Hulka Clips placed across the mesovarium and proper ligament of the ovary. CLINICAL RELEVANCE: Laparoscopic ovariectomy in the llama can be accomplished easily with few complications.


Subject(s)
Camelids, New World/surgery , Laparoscopy/veterinary , Ovariectomy/veterinary , Animals , Evaluation Studies as Topic , Female , Follow-Up Studies , Head-Down Tilt , Intraoperative Complications/veterinary , Ovariectomy/methods , Postoperative Complications/veterinary , Prospective Studies , Surgical Instruments/veterinary
11.
J Comp Pathol ; 118(1): 51-5, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9500238

ABSTRACT

This report describes a malignant odontogenic neoplasm in a 7-year-old bull. The mass, involving the right mandible, was locally invasive and destructive. Histologically, it consisted of islands and cords of benign odontogenic epithelium, entrapped in a population of malignant mesenchymal cells. These morphological features are characteristic of ameloblastic fibrosarcoma in man, an odontogenic tumour not previously described in animals.


Subject(s)
Fibrosarcoma/veterinary , Mandibular Neoplasms/veterinary , Odontogenic Tumors/veterinary , Animals , Cattle , Fatal Outcome , Fibrosarcoma/pathology , Male , Mandibular Neoplasms/pathology , Odontogenic Tumors/pathology
12.
Vet Surg ; 26(5): 444-50, 1997.
Article in English | MEDLINE | ID: mdl-9381667

ABSTRACT

OBJECTIVE: To study the combined effects of intra-abdominal CO2 insufflation with changes in body position during laparoscopy in xylazine-ketamine-halothane anesthetized llamas. STUDY DESIGN: Prospective, controlled study. ANIMALS: Nine castrated, male llamas weighing 114 +/- 23 kg, 3 to 13 years old. METHODS: Three llamas (preliminary study [PS] group) were used to study the effect of right, lateral, dorsal, and left lateral recumbency on gas exchange and acid-base status. The other six (experimental study [ES] group) were used to study the combined effects of changes in body position and CO2 insufflation to an intraabdominal pressure of 10 to 12 mm Hg. Heart rate, respiratory rate, and indirect arterial blood pressures (systolic [SAP], mean [MAP], and diastolic [DAP]) were recorded every 5 minutes during anesthesia. Arterial blood gases (PaO2 and PaCO2) and acid-base status (pHa and HCO3-) were measured immediately after induction of anesthesia and before each change of position. RESULTS: In the PS group, significant decreases in SAP, MAP and PaCO2 and increases in PaO2 and pHa were observed when the llamas were turned from right lateral to dorsal recumbency. Values for HCO3- were lower than the postinduction values, but they remained unaffected by the changes in position. In the ES group, values for MAP were significantly lower when the llamas were placed in dorsal and left lateral recumbency than those observed during right lateral recumbency. Arterial O2 tension during right lateral recumbency was lower but returned to preinsufflation values when the llamas were placed in the dorsal position. All llamas recovered uneventfully within 30 minutes after termination of anesthesia. CONCLUSIONS: Insufflation of CO2 and changing body position induce minor and transient changes in cardiovascular and respiratory function. CLINICAL RELEVANCE: Laparoscopy with mild intra-abdominal CO2 insufflation (10 to 12 mm Hg) can be used safely in spontaneously breathing llamas anesthetized with xylazine, ketamine, and halothane.


Subject(s)
Acid-Base Equilibrium/physiology , Camelids, New World/physiology , Carbon Dioxide/blood , Carbon Dioxide/pharmacology , Insufflation/veterinary , Oxygen/blood , Posture/physiology , Unconsciousness/veterinary , Acid-Base Equilibrium/drug effects , Anesthesia/methods , Anesthesia/veterinary , Anesthetics/pharmacology , Anesthetics, Dissociative/pharmacology , Anesthetics, Inhalation/pharmacology , Animals , Bicarbonates/blood , Blood Gas Analysis/methods , Blood Gas Analysis/veterinary , Blood Pressure/drug effects , Blood Pressure/physiology , Camelids, New World/blood , Carbon Dioxide/administration & dosage , Halothane/pharmacology , Heart Rate/drug effects , Heart Rate/physiology , Hydrogen-Ion Concentration , Insufflation/methods , Ketamine/pharmacology , Laparoscopy/methods , Laparoscopy/veterinary , Male , Prospective Studies , Pulmonary Ventilation/physiology , Respiration/drug effects , Respiration/physiology , Unconsciousness/blood , Unconsciousness/physiopathology , Xylazine/pharmacology
13.
Vet Clin North Am Equine Pract ; 13(2): 377-92, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9290190

ABSTRACT

Rectal tears have important medicolegal implications, and severe tears have a poor prognosis. Prompt diagnosis, immediate application of first aid measures, early referral, use of appropriate definitive treatments, and aggressive aftercare will improve the prognosis. The grade of rectal injury will determine the definitive treatment, but there are few guidelines to determine which treatment is best in each case. Bypass procedures, such as colostomy and indwelling rectal liner, have their own advantages and disadvantages, but can facilitate healing and prevent life-threatening complications if they are used with minimum delay. Suture of the tear can be difficult, but should be attempted to hasten healing and to prevent progression of the tear to a more severe injury.


Subject(s)
Horses/injuries , Rectum/injuries , Rectum/surgery , Animals , Colostomy/veterinary , First Aid/veterinary , Palpation/adverse effects , Palpation/veterinary , Postoperative Care/veterinary , Postoperative Complications/veterinary , Prognosis , Wounds and Injuries/etiology , Wounds and Injuries/surgery , Wounds and Injuries/veterinary
14.
Am J Vet Res ; 57(10): 1488-96, 1996 Oct.
Article in English | MEDLINE | ID: mdl-8896690

ABSTRACT

OBJECTIVE: To evaluate the ability of hyaluronic acid (HA), with and without transforming growth factor beta (1GF-beta), to stabilize the catabolic processes associated with atrophy of articular cartilage. ANIMALS: 20 adult, skeletally normal, hound-type dogs. PROCEDURE: Dogs (20 to 30 kg) were randomly assigned to 1 of 5 groups. One group served as untreated controls. Bivalve casts were placed on the left hind limbs of the remaining 16 dogs to limit weightbearing and motion of the limb for 92 days. One group served as the cast control. Beginning on day 56, 3 groups received aseptic intra-articular injections in the left stifles of either 5 mg of HA or 5 mg of HA containing either 20 or 50 micrograms of TGF-beta. Intraarticular injections were repeated at 4-day intervals until the end of the study. Or day 92, stifles were harvested at necroscropy. Medial femoral condyle were histologically processed, and the articular cartilage was stained for the presence of proteoglycans, stromelysin, tumor necrosis facto (TNF) alpha, and TNF receptors (p55 and p75). RESULTS: Decreased metachromasia was evident in the cartilage matrix of all cast groups, with the smallest decrease in the HA-treated group. Stromelysin was immunolocalized in articular cartilage of the cast (left) limbs of cast control and both HA/TGF-beta-treated groups. TNF-alpha was localized in articular cartilage of all cast (left) and right limbs, except those of the HA-treated group. Receptors for TNF were observed in both limbs of untreated control and cast control groups and cast limbs of HA/TGF-3-treated groups. The receptors were not localized in the right limbs of the HA with or without TGF-beta-treated groups. TGF-beta did not decrease stromelysin or TNF-alpha or receptors at the doses used. CONCLUSIONS: HA may mediate a chondrostabilizing influence on articular cartilage by down-regulating TNF-alpha importantly. HA appeared to exert its inhibitory influence on TNF-alpha, as well as stromelysin and TNF receptors, on a systemic basis. CLINICAL RELEVANCE: Results provide insight into the mode of action of HA as a therapeutic agent for arthritis and its stabilizing influence on cartilage metabolism.


Subject(s)
Cartilage, Articular/pathology , Hyaluronic Acid/pharmacology , Matrix Metalloproteinase 3/metabolism , Receptors, Tumor Necrosis Factor/metabolism , Transforming Growth Factor beta/pharmacology , Tumor Necrosis Factor-alpha/metabolism , Animals , Antibodies , Atrophy , Cartilage, Articular/drug effects , Cartilage, Articular/metabolism , Dogs , Enzyme-Linked Immunosorbent Assay , Female , Hindlimb , Immobilization , Immunohistochemistry , Male , Matrix Metalloproteinase 3/analysis , Receptors, Tumor Necrosis Factor/analysis , Stifle , Tumor Necrosis Factor-alpha/analysis
15.
J Am Vet Med Assoc ; 209(3): 650-2, 1996 Aug 01.
Article in English | MEDLINE | ID: mdl-8755990

ABSTRACT

A 14-month-old 40-kg Anglo-Nubian buck was referred for erection failure after recurrent bouts of obstructive urolithiasis. Previous episodes of obstructive urolithiasis were treated successfully with amputation of the urethral process, followed by an exploratory celiotomy and insertion of an indwelling Foley catheter into the bladder. The buck had been capable of intromission prior to the most recent episode of urethral obstruction. The results of physical examination and electroejaculation did not reveal any abnormalities, except failure to achieve erection. Contrast radiography of the corpus cavernosum penis (CCP), however, revealed failure of contrast media to migrate proximal to the sigmoid flexure. A diagnosis of obstruction of the CCP caused by cavernositis and fibrosis secondary to urolithiasis was made. A poor prognosis was given for breeding soundness. Although breeding soundness following obstructive urolithiasis may be enhanced by surgical intervention, impotence may result from obstruction of blood flow through the CCP secondary to urethral rupture or urethritis.


Subject(s)
Goat Diseases/etiology , Impotence, Vasculogenic/veterinary , Urinary Calculi/veterinary , Amputation, Surgical/veterinary , Animals , Goat Diseases/diagnostic imaging , Goat Diseases/surgery , Goats , Impotence, Vasculogenic/etiology , Male , Penis/diagnostic imaging , Radiography , Recurrence , Urethra/surgery , Urinary Calculi/complications , Urinary Calculi/surgery , Urinary Catheterization/veterinary
16.
Am J Vet Res ; 57(7): 1080-93, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8807026

ABSTRACT

OBJECTIVES: To use lipopolysaccharide (LPS) to create synovitis in the midcarpal joint of ponies, and to assess the morphologic, histochemical, and immunohistochemical effects of synovitis on articular cartilage of the third carpal bone. ANIMALS: 2- to 3-year-old ponies, 6 control (group 1) and 6 treated (group 2). PROCEDURE: Synovitis was induced in 1 midcarpal joint of group-2 ponies by intra-articular injections of LPS (0.02 micrograms/kg of body weight), morphine (0.1 mg/kg), and saline solution (group 2a) and a morphine and saline solution alone in the contralateral midcarpal joint (group 2b). Articular cartilage sections and attached synovial membrane from the third carpal bones were examined by immunohistochemical distribution of interleukin 1 beta, tumor necrosis factor (TNF)-alpha, TNF receptors (P55, P75) and 3-B-3(-) epitopes, and by localization of proteoglycans (metachromatic staining). Proteoglycan extracts were assessed by metachromatic staining or western blotting and immunohistochemical staining, using anti-3-B- antibodies. RESULTS: Enhanced immunoreactivity for the cytokines and receptors was found in inflamed synovial membrane and noncalcified cartilage (group 2a more than 2b). Metachromasia of the noncalcified cartilage was greater in group-1 than in group-2a and group-2b specimens. In group 2a, chondrocyte hypertrophy and enhanced immunoreactivity for 3-B-3(-) epitope in areas of increased cytokine immunoreactivity suggested possible phenotypic change of the chondrocytes in response to synovitis. Immunohistochemical analysis by western blotting of proteoglycan extracts indicated strong 3-B-3(-) epitope immunolocalization in group-2a, weaker staining in group-2b, and barely detectable stain in group-1 specimens, which correlated with in situ immunolocalization. CONCLUSIONS: Intra-articular administration of LPS may be used to induce a synovial environment conductive to increased immunoreactivity of interleukin 1 beta, TNF-alpha, and its receptors in equine synovial membrane and articular cartilage. These cytokines may be involved in the early phenotypic change of chondrocytes that is believed to occur in osteoarthritis and is characterized in this study by enhanced 3-B-3(-) epitope immunoreactivity and chondrocyte hypertrophy.


Subject(s)
Arthritis, Rheumatoid/pathology , Cartilage, Articular/pathology , Synovial Membrane/pathology , Synovitis/pathology , Animals , Antigens, CD/analysis , Arthritis, Rheumatoid/chemically induced , Blotting, Western , Carpal Bones , Cartilage, Articular/immunology , Epitopes , Horses , Hypertrophy , Immunohistochemistry/methods , Interleukin-1/analysis , Joints/immunology , Joints/pathology , Lipopolysaccharides , Receptors, Tumor Necrosis Factor/analysis , Receptors, Tumor Necrosis Factor, Type I , Receptors, Tumor Necrosis Factor, Type II , Synovial Membrane/immunology , Synovitis/immunology , Tumor Necrosis Factor-alpha/analysis
17.
J Am Vet Med Assoc ; 208(2): 261-2, 1996 Jan 15.
Article in English | MEDLINE | ID: mdl-8567386

ABSTRACT

OBJECTIVE: To compare a prescrotal castration technique with the conventional bilateral scrotal incision technique for castration of llamas. DESIGN: Prospective randomized controlled trial. ANIMALS: 10 clinically normal, sexually intact male llamas. PROCEDURE: Five llamas were castrated by use of a 5-cm skin incision located 2 to 3 cm lateral to the ventral midline and approximately 15 cm cranial to the scrotum, which was closed with absorbable suture material to allow primary healing. Five other llamas were castrated via a more conventional technique, with a 5-cm scrotal incision positioned directly over each testis, which was allowed to heal by second intention. RESULTS: The prescrotal technique required significantly more time to complete; however, no additional anesthesia was required to complete the longer procedure. Llamas castrated with the prescrotal technique required less aftercare and had less incisional pain when the area was palpated. CLINICAL IMPLICATIONS: Both techniques are safe and effective. Some clients, however, find the prescrotal technique more aesthetically acceptable. The prescrotal technique may be more clinically important where fly control is difficult.


Subject(s)
Camelids, New World/surgery , Orchiectomy/veterinary , Animals , Male , Orchiectomy/economics , Orchiectomy/methods , Pain, Postoperative/veterinary , Postoperative Care/veterinary , Prospective Studies , Scrotum/surgery , Suture Techniques/veterinary , Time Factors
18.
J Am Vet Med Assoc ; 208(1): 111-6, 1996 Jan 01.
Article in English | MEDLINE | ID: mdl-8682698

ABSTRACT

OBJECTIVE: To describe 3 laparoscopic approaches for, and the normal laparoscopic anatomy of, the abdomen in adult llamas and to evaluate the effects of laparoscopy in those llamas. DESIGN: Prospective clinical trial. ANIMALS: Six adult castrated male llamas. PROCEDURE: After induction of general anesthesia, 3 surgical approaches to the abdomen were performed: left paralumbar, ventral midline, and right paralumbar. The abdomen was systematically examined, and anatomic features described. After recovery from anesthesia, all llamas were examined daily for 10 days and CBC was repeated 24, 72, and 120 hours after laparoscopy. RESULTS: Laparoscopy was successfully performed in all llamas by use of the ventral midline and right paralumbar approaches. The laparoscope was inadvertently placed into the left retroperitoneal space in 1 of the 6 llamas when the left paralumbar approach was used. Also, hemorrhage into the abdomen limited the view from the left side in another llama. Various approaches allowed viewing of the first and third forestomach compartments, liver, spleen, kidneys, small intestine, ileum, proximal loop of the ascending colon, spiral colon, and urinary bladder. Postoperative findings included subcutaneous emphysema and edema. Mean WBC count peaked 24 hours after surgery (mean, 23,500 cells/microliter). Generally, neutrophil count increased and lymphocyte count decreased during the 120 hours after surgery. CLINICAL IMPLICATIONS: Laparoscopy may be used for differentiation of medical and surgical lesions in the abdomen of llamas. The site for laparoscopy should be chosen on the basis of the most likely site of the suspected lesion.


Subject(s)
Abdomen/anatomy & histology , Camelids, New World/surgery , Laparoscopy/veterinary , Abdomen/surgery , Animals , Camelids, New World/anatomy & histology , Laparoscopy/methods , Male
20.
J Am Vet Med Assoc ; 204(3): 420-1, 1994 Feb 01.
Article in English | MEDLINE | ID: mdl-8150700

ABSTRACT

Bilateral fractures of the mandible were identified in a 9-month-old mixed-breed beef steer, used for show, that had been hit by a car 8 hours prior to admission. The fractures involved the left interdental space and the premolar region of the right hemimandible. For maximal cosmesis, fractures were repaired via internal fixation by use of a medially positioned broad dynamic-compression plate and cerclage wire. A medial approach served to protect more laterally located vascular and nervous structures. The wider intermandibular space in cattle, compared with that in horses, made the medial approach and plate placement feasible.


Subject(s)
Cattle/injuries , Fracture Fixation, Internal/veterinary , Mandibular Fractures/surgery , Accidents, Traffic , Animal Feed , Animals , Bone Plates/veterinary , Bone Screws/veterinary , Bone Wires/veterinary , Cattle/surgery , Eating , Male
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