Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Ann Clin Microbiol Antimicrob ; 9: 21, 2010 Aug 04.
Article in English | MEDLINE | ID: mdl-20684778

ABSTRACT

BACKGROUND: Pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification of P. aeruginosa from positive blood cultures (BCs). METHODS: Over the period August 2008 to June 2009, 100 BC bottles positive for gram-negative bacilli were tested in order to evaluate performances of the qPCR technique with conventional methods as gold standard (i.e. culture and phenotypic identification). RESULTS: Thirty-three strains of P. aeruginosa, 53 strains of Enterobactericaeae, nine strains of Stenotrophomonas maltophilia and two other gram-negative species were isolated while 3 BCs were polymicrobial including one mixture containing P. aeruginosa. All P. aeruginosa clinical isolates were detected by qPCR except a single strain in mixed culture. Performances of the qPCR technique were: specificity, 100%; positive predictive value, 100%; negative predictive value, 98.5%; and sensitivity, 97%. CONCLUSIONS: This reliable technique may offer a rapid (<1.5 h) tool that would help clinicians to initiate an appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of this novel strategy as compared to phenotypic methods.


Subject(s)
Blood/microbiology , DNA, Bacterial/analysis , Polymerase Chain Reaction/methods , Pseudomonas Infections/diagnosis , Pseudomonas aeruginosa/isolation & purification , Bacteriological Techniques/methods , Enterobacteriaceae/isolation & purification , Gram-Negative Bacteria/isolation & purification , Humans , Pseudomonas aeruginosa/genetics , Sensitivity and Specificity , Sequence Analysis, DNA , Stenotrophomonas/isolation & purification
2.
Ann Clin Microbiol Antimicrob ; 7: 19, 2008 Sep 25.
Article in English | MEDLINE | ID: mdl-18817558

ABSTRACT

Brachyspira pilosicoli is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. Here, we describe the case of a B. pilosicoli spirochetemia in a 53-year-old male patient suffering from cardiogenic shock. This fastidious bacterium was isolated from blood, likely after translocation from the intestinal tract. Blood cultures were positive after 5 days of incubation (one day after the patient's death), highlighting the problem of the recovery of such type of fastidious bacterium. Identification was achieved by molecular methods (16S rRNA sequencing). A review of the English literature found only 8 cases of bacteremia caused by B. pilosicoli, mostly in immunocompromised or critically ill patients. Finally, difficulties in rapid and accurate diagnosis of B. pilosicoli bloodstream infections, in vitro antimicrobial susceptibility of human clinical isolates, and therapeutic options are discussed.


Subject(s)
Bacteremia/microbiology , Brachyspira/isolation & purification , Gram-Negative Bacterial Infections/microbiology , Animals , Blood/microbiology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatal Outcome , Humans , Male , Middle Aged , Sequence Analysis, DNA , Shock, Cardiogenic/complications
SELECTION OF CITATIONS
SEARCH DETAIL
...