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1.
Biomolecules ; 7(3)2017 08 01.
Article in English | MEDLINE | ID: mdl-28763009

ABSTRACT

The cytoplasmic polyadenylation element binding protein (CPEB) homologue Orb2 is a functional amyloid that plays a key regulatory role for long-term memory in Drosophila. Orb2 has a glutamine, histidine-rich (Q/H-rich) domain that resembles the Q/H-rich, metal binding domain of the Hpn-like protein (Hpnl) found in Helicobacter pylori. In the present study, we used chromatography and isothermal titration calorimetry (ITC) to show that the Q/H-rich domain of Orb2 binds Ni2+ and other transition metals ions with µM affinity. Using site directed mutagenesis, we show that several histidine residues are important for binding. In particular, the H61Y mutation, which was previously shown to affect the aggregation of Orb2 in cell culture, completely inhibited metal binding of Orb2. Finally, we used thioflavin T fluorescence and electron microscopy images to show that Ni2+ binding induces the aggregating of Orb2 into structures that are distinct from the amyloid fibrils formed in the absence of Ni2+. These data suggest that transition metal binding might be important for the function of Orb2 and potentially long-term memory in Drosophila.


Subject(s)
Drosophila Proteins/genetics , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Histidine/metabolism , Nickel/metabolism , Transcription Factors/genetics , Transcription Factors/metabolism , mRNA Cleavage and Polyadenylation Factors/genetics , mRNA Cleavage and Polyadenylation Factors/metabolism , Animals , Binding Sites , Calorimetry , Circular Dichroism , Drosophila Proteins/chemistry , Drosophila melanogaster/genetics , Glutamine/metabolism , Microscopy, Electron , Mutagenesis, Site-Directed , Protein Binding , Transcription Factors/chemistry , mRNA Cleavage and Polyadenylation Factors/chemistry
2.
Biophys J ; 113(1): 37-47, 2017 Jul 11.
Article in English | MEDLINE | ID: mdl-28700922

ABSTRACT

Lipid membranes interact with and influence the aggregation of many amyloid-forming proteins. Orb2 is a cytoplasmic polyadenylation element-binding protein homolog in Drosophila melanogaster that forms functional amyloids necessary for long-term memory. One isoform, Orb2A, has a unique N-terminus that has been shown to be important for the formation of amyloid-like aggregates and long-term memory in vivo. Orb2A is also found enriched in the synaptic membrane fraction. Our sequence and hydropathy analysis suggests that it can form an amphipathic helix, which is ideal for lipid membrane interaction. We used circular dichroism and site-directed spin labeling coupled with electron paramagnetic resonance to test the first 88 amino acids of Orb2A for lipid interaction. We show that Orb2A1-88 interacts with anionic lipid membranes using an amphipathic helix at its unique N-terminus. This interaction depends on the charge of the lipid membrane and the degree of membrane curvature. We used transmission electron microscopy and electron paramagnetic resonance to show that the presence of anionic small unilamellar vesicles inhibits amyloid fibril formation by Orb2A. This inhibition by anionic membranes could be a potential mechanism regulating Orb2A amyloid formation in vivo.


Subject(s)
Amyloid/metabolism , Drosophila Proteins/metabolism , Phosphatidylcholines/chemistry , Phosphatidylglycerols/chemistry , Phosphatidylserines/chemistry , Transcription Factors/metabolism , Unilamellar Liposomes/chemistry , mRNA Cleavage and Polyadenylation Factors/metabolism , Amino Acid Sequence , Amyloid/chemistry , Animals , Binding Sites , Circular Dichroism , Drosophila Proteins/chemistry , Drosophila Proteins/genetics , Drosophila melanogaster , Electron Spin Resonance Spectroscopy , Escherichia coli , Microscopy, Electron, Transmission , Protein Isoforms/chemistry , Protein Isoforms/genetics , Protein Isoforms/metabolism , Protein Structure, Secondary , Surface Properties , Transcription Factors/chemistry , Transcription Factors/genetics , mRNA Cleavage and Polyadenylation Factors/chemistry , mRNA Cleavage and Polyadenylation Factors/genetics
3.
Sci Rep ; 6: 38265, 2016 12 06.
Article in English | MEDLINE | ID: mdl-27922050

ABSTRACT

Orb2 is a functional amyloid that plays a key role in Drosophila long-term memory formation. Orb2 has two isoforms that differ in their N-termini. The N-terminus of the A isoform (Orb2A) that precedes its Q-rich prion-like domain has been shown to be important for Orb2 aggregation and long-term memory. However, besides the fact that it forms fibrillar aggregates, structural information of Orb2 is largely absent. To understand the importance of the N-terminus of Orb2A and its relation to the fibril core, we recorded solid-state NMR and EPR data on fibrils formed by the first 88 residues of Orb2A (Orb2A88). These data show that the N-terminus of Orb2A not only promotes the formation of fibrils, but also forms the fibril core of Orb2A88. This fibril core has an in-register parallel ß-sheet structure and does not include the Q-rich, prion-like domain of Orb2. The Q-rich domain is part of the unstructured region, which becomes increasingly dynamic towards the C-terminus.


Subject(s)
Drosophila Proteins/chemistry , Drosophila Proteins/metabolism , Transcription Factors/chemistry , Transcription Factors/metabolism , mRNA Cleavage and Polyadenylation Factors/chemistry , mRNA Cleavage and Polyadenylation Factors/metabolism , Amyloid/chemistry , Amyloid/metabolism , Drosophila Proteins/genetics , Electron Spin Resonance Spectroscopy , Magnetic Resonance Spectroscopy , Protein Domains , Protein Isoforms , Transcription Factors/genetics , mRNA Cleavage and Polyadenylation Factors/genetics
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