Antibiofilm and Protein-Repellent Polymethylmethacrylate Denture Base Acrylic Resin for Treatment of Denture Stomatitis.

Candida albicans (C. albicans) biofilm is a common etiological factor in denture stomatitis. The purpose of this study was to investigate the effects of incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC) as a protein repellent into a new high-impact denture acrylic (HIPA) resin on the surface roughness, solution pH, and C. albicans biofilm adhesion to the denture base. The new acrylic denture resin base was formulated by mixing MPC into HIPA resin at mass fractions of 1.5%, 3%, and 4.5%. Surface roughness was measured using a Mitutoyo surface roughness tester. C. albicans biofilm growth and viability were assessed via colony forming unit counts. The pH of the biofilm growth medium was measured using a digital pH meter. Adding MPC to the HIPA resin at percentages of 1.5% and 3% increased the roughness values significantly (p < 0.05), while adding 4.5% MPC resulted in no difference in roughness values to that of the control group (p > 0.05). All experimental groups demonstrated neutral pH values (pH ≅ 7) and were not significantly different from each other (p > 0.05). Incorporating 2-methacryloyloxyethyl phosphorylcholine at 4.5% resulted in a significant (≅1 log) colony-forming unit reduction compared with the control group with 0% MPC (p < 0.05). A fungal-retarding denture acrylic resin was developed through the incorporation of MPC for its protein-repelling properties. This newly developed denture acrylic material has the potential to prevent oral microbial infections, such as denture stomatitis.

The Fit Accuracy of Removable Partial Denture Metal Frameworks Using Conventional and 3D Printed Techniques: An In Vitro Study.

AIM: To evaluate the accuracy of removable partial denture (RPD) metal frameworks fabricated by the conventional lost-wax (CLW) technique and those made by the selective laser melting (SLM). MATERIALS AND METHODS: A dentoform of a mandibular Kennedy class III, modification 1 dental arch were surveyed, and rest seats were prepared on the abutment teeth. The dentoform was then duplicated into a metal die which was used as a reference model. Thirty RPD metal frameworks were fabricated by two techniques; fifteen for each technique. Polyvinyl siloxane (PVS) impression material was painted on the intaglio surface of the rests of each framework which is then seated on the reference die. PVS specimens that represent the gap under the rest were measured in four zones: buccal, lingual, marginal and central by a single examiner using a digital microscope at 50x in micrometers. RESULTS: Comparison between the two techniques for each abutment tooth revealed that the CLW technique had better fit in one tooth, while the SLM technique showed a better fit in two teeth. Regarding the edentulous span length within the SLM technique, the long edentulous span had a significantly better fit. When comparing the four measured rest zones, it was found that in the CLW technique group, the marginal zone had the highest fit accuracy while the lingual zone showed the lowest fit accuracy. In the SLM group, the central zone had the best fit and the buccal zone had the worst fit. CONCLUSION: RPD frameworks fabricated using the SLM technique showed better fit accuracy than those made by the CLW technique, however, the difference was not statistically significant. CLINICAL SIGNIFICANCE: SLM is a promising technique for the fabrication of RPD frameworks in routine clinical practice.