Neuronal Cell Death Induced by Mechanical Percussion Trauma in Cultured Neurons is not Preceded by Alterations in Glucose, Lactate and Glutamine Metabolism.

Traumatic brain injury (TBI) is a devastating neurological disorder that usually presents in acute and chronic forms. Brain edema and associated increased intracranial pressure in the early phase following TBI are major consequences of acute trauma. On the other hand, neuronal injury, leading to neurobehavioral and cognitive impairments, that usually develop months to years after single or repetitive episodes of head trauma, are major consequences of chronic TBI. The molecular mechanisms responsible for TBI-induced injury, however, are unclear. Recent studies have suggested that early mitochondrial dysfunction and subsequent energy failure play a role in the pathogenesis of TBI. We therefore examined whether oxidative metabolism of (13)C-labeled glucose, lactate or glutamine is altered early following in vitro mechanical percussion-induced trauma (5 atm) to neurons (4-24 h), and whether such events contribute to the development of neuronal injury. Cell viability was assayed using the release of the cytoplasmic enzyme lactate dehydrogenase (LDH), together with fluorescence-based cell staining (calcein and ethidium homodimer-1 for live and dead cells, respectively). Trauma had no effect on the LDH release in neurons from 1 to 18 h. However, a significant increase in LDH release was detected at 24 h after trauma. Similar findings were identified when traumatized neurons were stained with fluorescent markers. Additionally (13)C-labeling of glutamate showed a small, but statistically significant decrease at 14 h after trauma. However, trauma had no effect on the cycling ratio of the TCA cycle at any time-period examined. These findings indicate that trauma does not cause a disturbance in oxidative metabolism of any of the substrates used for neurons. Accordingly, such metabolic disturbance does not appear to contribute to the neuronal death in the early stages following trauma.

Neuron-glia interactions in glutamatergic neurotransmission: roles of oxidative and glycolytic adenosine triphosphate as energy source.

Glutamatergic neurotransmission accounts for a considerable part of energy consumption related to signaling in the brain. Chemical energy is provided by adenosine triphosphate (ATP) formed in glycolysis and tricarboxylic acid (TCA) cycle combined with oxidative phosphorylation. It is not clear whether ATP generated in these pathways is equivalent in relation to fueling of the energy-requiring processes, i.e., vesicle filling, transport, and enzymatic processing in the glutamatergic tripartite synapse (the astrocyte and pre- and postsynapse). The role of astrocytic glycogenolysis in maintaining theses processes also has not been fully elucidated. Cultured astrocytes and neurons were utilized to monitor these processes related to glutamatergic neurotransmission. Inhibitors of glycolysis and TCA cycle in combination with pathway-selective substrates were used to study glutamate uptake and release monitored with D-aspartate. Western blotting of glyceraldehyde-3-P dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) was performed to determine whether these enzymes are associated with the cell membrane. We show that ATP formed in glycolysis is superior to that generated by oxidative phosphorylation in providing energy for glutamate uptake both in astrocytes and in neurons. The neuronal vesicular glutamate release was less dependent on glycolytic ATP. Dependence of glutamate uptake on glycolytic ATP may be at least partially explained by a close association in the membrane of GAPDH and PGK and the glutamate transporters. It may be suggested that these enzymes form a complex with the transporters and the Na(+) /K(+) -ATPase, the latter providing the sodium gradient required for the transport process.

The FcgammaRIIa polymorphism in patients with chronic kidney graft rejection.

The FcgammaRIIa receptors, which provide a crucial link between cellular and humoral components of the immune response, display allelic polymorphism. Individuals are homozygous for either arginine 131 (RR131) or histidine 131 (HH131) or are heterozygous for these two alleles (RH131). The HH131 genotype binds human IgG2 with high RR131 with low, and RH131 with intermediate affinity. The aim of the study was to evaluate the FcgammaRIIa polymorphism in patients with chronic kidney graft rejection. The study included 121 renal transplant recipients: 53 patients with long-term stable graft function and 68 with chronic allograft rejection. The distribution of FcgammaRIIa genotypes in patients with chronic kidney graft rejection did not differ significantly from that in patients with stable graft function. The results suggest that the FcgammaRIIa polymorphism is not an important genetic risk factor for chronic rejection of kidney allografts.

Role of astrocytic transport processes in glutamatergic and GABAergic neurotransmission.

The fine tuning of both glutamatergic and GABAergic neurotransmission is to a large extent dependent upon optimal function of astrocytic transport processes. Thus, glutamate transport in astrocytes is mandatory to maintain extrasynaptic glutamate levels sufficiently low to prevent excitotoxic neuronal damage. In GABA synapses hyperactivity of astroglial GABA uptake may lead to diminished GABAergic inhibitory activity resulting in seizures. As a consequence of this the expression and functional activity of astrocytic glutamate and GABA transport is regulated in a number of ways at transcriptional, translational and post-translational levels. This opens for a number of therapeutic strategies by which the efficacy of excitatory and inhibitory neurotransmission may be manipulated.

The Fc gamma RIIa polymorphism in patients with acute kidney graft rejection.

The Fc gamma RIIa receptors are the most widely distributed of the three classes of Fc receptors and expresses an allelic polymorphism. This polymorphism may modulate the immune response and may be a factor for some diseases. The aim of the study was to evaluate the association between the Fc gamma RIIa polymorphism and acute kidney graft rejection. The study was carried out in 115 kidney allograft recipients. The frequency of acute kidney graft rejection was similar in relation to Fc gamma RIIa genotypes. We suggest that Fc gamma RIIa polymorphism is not important risk factor for acute kidney graft rejection susceptibility.

Incidence of nutritional risk and causes of inadequate nutritional care in hospitals.

BACKGROUND AND AIMS: Many patients in hospitals are undernourished and nutritional care is inadequate in most hospitals. The aim of this investigation was to gain insight into how this situation could be improved. METHODS: Seven hundred and fifty randomly selected patients were screened at admission in three hospitals and surveyed during their entire hospitalization. Each time a patient was not treated according to a clearly defined nutritional standard, the nurse responsible for the patient was interviewed about possible reasons according to preformed questionnaires. RESULTS: The investigators found that 22% of the patients were nutritionally at-risk, and that only 25% of these patients received an adequate amount of energy and protein. The departments had only screened for nutritional problems in 60% of the cases. Only 47% of the patients, who the departments judged to be at-risk patients, had a nutrition plan worked out, and only about 30% of the at-risk patients were monitored by the departments by recording of dietary intake and/or body weight. The main causes for inadequate nutritional care were lack of instructions to deal with these problems, and lack of basic knowledge with respect to dietary requirements and practical aspects of the hospital's food provision. Patient-related aspects and the system of food provision also contributed, but only to a small degree. CONCLUSIONS: These findings form the basis of the strategy to improve nutritional care in these hospitals.

Sensory and chemical analysis of cooked porcine meat patties in relation to warmed-over flavour and pre-slaughter stress.

Two independent sensory profiles were carried out to evaluate warmed-over flavour (WOF) development in cooked, chill-stored and reheated pork patties. The patties were derived from the Musculus semimembranosus of animals subjected to different pre-slaughter stress treatments. All patties were stored in oxygen permeable bags at 4°C for 0, 1, 2, 3 and 5 days to facilitate WOF development. In addition, thiobarbituric acid reactive substances (TBARS), conjugated dienes, pH, water content, total lipids and the fatty acid compositions of phosphatidyl choline (PC), phosphatidyl ethanolamine (PE) and total lipids, were measured in the cooked meat patties. A data analytical strategy involving Analysis of Variance-Partial Least Squares Regression (ANOVA-PLSR), to determine relationships between the design variables (WOF and pre-slaughter stress) and the sensory-chemical data, and PLSR to elucidate predictive links between the sensory and chemical data was utilised. WOF was found to involve the development of lipid oxidation derived nuance off-flavour and odour notes, e.g. rancid-like flavour and linseed oil-like odour, in association with a concurrent decrease in cooked pork meat-like flavour. The reduction in "meatiness", over the initial days, 0-2 of WOF development was attributed to the degradation of both, unstable sulfur-containing amino acids in meat proteins and sulfur-containing "meaty" aroma compounds. Whereas, at the later days, 3-5 of WOF development the "meaty" loss was ascribed to perceptual masking by lipid oxidation products. TBARS and conjugated dienes were found to be significant (P<0.05) predictors of the sensory terms related to the lipid oxidation aspect of WOF. Whilst the polyunsaturated fatty acid (PUFAs) contents of PE, PC and the total lipids were found to decrease with WOF development, reflecting their loss in lipid oxidation reactions. The sensory variation related to pre-slaughter stress appeared to be distinct from WOF variation and was described by a sour to sweet taste continuum. However, interactions were noted that indicated increasing pre-slaughter stress resulted in a decreased sensory perception of WOF. Moreover, pH and water content were found to significantly (P<0.05) predict the sensory effects resulting from pre-slaughter stress.

Warmed-over flavour in porcine meat - a combined spectroscopic, sensory and chemometric study.

The effectiveness of applying rapid spectral techniques in the prediction of meat quality in relation to pre-slaughter stress and warmed-over flavour (WOF) was investigated. The effect of pre-slaughter stress on the development of WOF is a relatively new area of interest in WOF research. The present study investigated the relationship between pre-slaughter stress and WOF characteristics (after 0 to 5 days' storage) in porcine meat as evaluated by chemical, sensory and spectroscopic methods. Sensory evaluation, visual reflectance spectroscopy (VIS) and low-field (1)H NMR (LF-NMR) proved efficient in describing the different stress groups and the storage period, whereas fluorescence spectroscopy and the TBARS test were only able to follow WOF during storage. Near Infrared Reflectance (NIR) and Raman scatter showed a very weak relationship to pre-slaughter stress and levels of WOF during storage. Good correlations (up to r=0.93) of sensory terms were achieved with VIS and fluorescence spectroscopy and LF-NMR.

MR imaging of the wrist in carpal tunnel syndrome.

PURPOSE: To determine whether specific parameters measured on MR images correlated to electrophysiological changes in carpal tunnel syndrome (CTS). MATERIAL AND METHODS: Prospective clinical examinations were made of 20 patients with suspected CTS. We performed bilateral electrophysiological examinations of the median nerve and bilateral MR imaging of the wrists. RESULTS: The electrophysiological examination suggested median nerve entrapment in 18 wrists. These wrists were compared to the remaining 22 electrophysiologically normal wrists. In addition, we compared both wrists in 12 patients with unilateral symptoms of CTS without reference to the electrophysiological findings. We found no difference in specific MR parameters between the 2 groups. CONCLUSION: Neither symptoms nor electrophysiological findings in CTS were related to specific MR parameters.

[Prevalence of risk factors in cerebral ischemia]. / Forekomst af risikofaktorer ved cerebral iskaemi.

The prevalence of risk factors for ischaemic stroke is described in 270 patients with transient ischaemic attacks or ischaemic stroke. Smoking was the most common risk factor (46%) followed by hypertension (30%) and increased levels of serum cholesterol (26%). Diabetes mellitus, atrial fibrillation and earlier myocardial infarction were less common risk factors. Ninety-one patients (34%) had two or more risk factors. In conclusion, vascular risk factors are frequently present in patients with transient ischaemic attacks or ischaemic stroke. It is still not yet clarified if risk factor modification is of any value in prevention of stroke recurrence.

[Symptomatic treatment of amyotrophic lateral sclerosis]. / Symptomatisk behandling af amyotrofisk lateralsklerose.

During a twelve year period 58 patients with motor neurone disease were admitted to the Department of Neurology, Odense University Hospital. The medical records were reviewed and different aspects of symptomatic treatment for these patients were recorded retrospectively. After the first admission 55% of the patients received out-patient treatment, while 31% had no further contact to the Department of Neurology. Forty-nine patients developed bulbar symptoms. Of these patients 41% were referred to a laryngologist, 27% were referred to a speech therapist and 10% to a nutritionist. Seven patients had a gastrostomy, while feeding tube was used by at least six patients. At the time of follow up 49 patients had died, 63% in hospital. At least 22 patients were treated with morphine in the last period of their lives. In order to improve the symptomatic treatment in motor neurone disease we suggest that these patients are treated at the neurological departments by interdisciplinary teams with particular interest in motor neurone disease.

[A case of nephrotic syndrome in chronic glomerulonephritis with coexistent diffuse thrombophlebitis]. / Przypadek zespolu nerczycowego w przebiegu przewleklego zapalenia klebków nerkowych ze wspólistniejaca rozsiana zakrzepica zylna.

A case is described of nephrotic syndrome with coexistent thrombosis of the splenic vein and the common iliac veins. The possible pathological mechanism of tge disease and the therapeutic and diagnostic difficulties during this disease superimposed upon chronic glomerulonephritis are discussed.

[Early detection of diseases of the circulatory system in vocational school students with particular reference to the prevention of coronary disease and arterial hypertension]. / Wczesne wykrywanie chorób ukladu krazenia u mlodziezy szkól zawodowych ze szczególnym uwzglednieniem prewencji choroby wiencowej i nadcisnienia tetniczego.

A group of 201 boys of vocational schools living in an area of developing industry (Belchatów) and another area of developed industry (Lódz) were examined twice: at the age of 16-17 (I examination) and 14-18 months later (II examination). Incomplete right bundle-branch block and intraventricular conduction impairment have been demonstrated mainly in the resting ECG tracings (Table I). The risk factors of coronary heart disease were increasing with age, especially in boys of Belchatów area (Table II). Five boys with risk factors of coronary heart disease exhibited ischaemic type of postexertional ST segment changes (Table III).