[Changes in the migration and adhesive activity of polymorphonuclear leukocytes as 1 of the characteristics of Gram-negative bacteria isolated from patients with suppurative lung destruction]. / Izmenenie migratsionnoi i adgezivnoi aktivnosti polimorfnoiadernykh leikotsitov, kak odna iz kharakteristik gramotritsatel'nykh bakterii, vydelennykh ot bol'nykh s gnoinymi destruktsiiami legkikh.

The influence of Gram-negative bacteria on the migratory and adhesive activity of polymorphonuclear leukocytes (PMNL) in the peripheral blood of clinically normal donors has been studied by the specially developed method with the use of Boyden chambers. Pseudomonas and enterobacteria have been found to produce complex and various effects on the above-mentioned properties of PMNL. When incubated in fresh serum, Gram-negative bacteria are capable of enhancing the migratory activity of PMNL, this property being least pronounced in P. aeruginosa. The incubation of live bacteria from the authors' collection in the patients' sera or in sera obtained from normal donors and inactivated by heating induces no hemotaxis of PMNL, and P. aeruginosa strains even suppress it under such conditions. The isolated Gram-negative bacteria under study increase the number of highly adhesive PMNL in the population used in this investigation, but P. aeruginosa cultures do not produce such effect.

[Immunobiologic study of the role of heterogenetic antigens of uropathogenic strains of E. coli]. / Immunobiologicheskoe izuchenie roli geterogennykh antigenov uropatogennykh shtammov E. coli.

Analysis of 140 strains of E. coli isolated from patients with pyelonephritis has demonstrated heterogeneous antigens (in 44.3% of cases) related to ABO antigens against human red blood cells. 0(H)-like antigen occurred most frequently. The data obtained suggest that the presence of heterogeneous antigens in E. coli is associated with the formation of a latent subclinical form of pyelonephritis and with the decrease titers of antibacterial antibodies in patients.

[Microflora and its antibiotic sensitivity in chronic pharyngitis treated by cryotherapy]. / Issledovanie mikroflory i ee chuvstvitel'nosti k antibiotikam u bol'nykh khronicheskim faringitom, lechennykh kriovozdeistviem

Antibiotic sensitivity of the pharynx mucosa microflora in patients with chronic pharyngitis subjected to cryotherapy was studied. Examination of 36 patients showed that cryotherapy at a temperature of -1950 markedly decreased the number of the grown colonies, their qualitative characteristics being changed. In 10 days both the quantitative and the qualitative characteristics of the flora completely reduced. The cryotherapy did not change the virulence of the flora and its sensitivity to antibiotics.

[Comparative study of mycoplasmae and stable L-forms of bacteria]. / Sravnitel'noe izuchenie mikoplazm i stabil'nykh L-form bakterii

A comparative study of mycoplasmae and L-form bacteria was carried out. Both of them were well passaged on a special nutrient medium with the addition of thallium acetate, i.e. they were resistant to this factor. But, in difference from the L-form bacteria, mycoplasmae failed to be passaged on the serum-free medium; the cultures of the L-form bacteria were highly resistant to the action of osmotic stabilizers and to the pressure of the nutrient medium of 15 and 30 atm; as to mycoplasmae--they perished at such a high pressure. The majority of mycoplasmae were resistant to the action of osmotic shock, and L-form bacteria lost their viability in distilled water as soon as in 2 hours.

[Isolation of Mycoplasma from the urine of patients with chronic pyelonephritis]. / Vydelenie mikoplazm iz mochi bol'nykh khronicheskim pielonefritom

Bacteriological analysis of urine of 150 patients with chronic pyelonephritis was performed. As a result mycoplasma was isolated from urine of 25 patients. Mycoplasma and Coli bacteria or Proteus were isolated simultaneously from urine of 10 patients. Biochemical properties and sensitivity to antibiotics of 9 isolates were studied. The data provided recommendation of the urine analysis for the presence of mycoplasma.

[L-forms of bacteria in the urine of patients with chronic pyelonephritis]. / L-formy bakterii v moche bol'nykh khronicheskim pielonefritom

The authors present the data concerning the study of 200 patients suffering from chronic pyelonephritis; in 28 of these L-forms of bacteria were revealed in the urine. Of 46 L-cultures isolated from these patients 13 reversed into bacterial forms, 8 failed to reverse and were referred to the stable L-forms; the rest 25 L-cultures perished during the 8th--10th passage. This led to a supposition that the relapses and exacerbations of the infectious process in pyelonephritis were associated with the change of the L-forms into bacterial ones, and that the persistence of the L-forms in the kidney tissue promoted the maintenance of the chronic process.