ABSTRACT
The results of the examination of association of polymorphisms of DNA repair genes and chromosomal aberrations in lung cancer patients are discussed. A significant positive association between the hOGG1 G/G genotypes, XPD G/G genotype and lung cancer was found. The hOGG1 C/C genotypes were significantly negatively associated with lung cancer. The patient chromosomal aberration frequencies were significantly higher than in control. Carriers of all APE1 and XPD genotypes, XRCC1 G/G genotype, ADPRT T/T genotype, hOGG1 C/C and Ser/Cys genotypes had statistically significant differences in the level of the chromosomal aberrations between patient and control groups. Statistically significant differences in the level of chromosomal aberrations between XPD T/T and G/G genotype of lung cancer patients were observed.
Subject(s)
Adenocarcinoma/genetics , Chromosome Aberrations , DNA Repair/genetics , Lung Neoplasms/genetics , Adenocarcinoma/pathology , DNA Glycosylases/genetics , DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics , DNA-Binding Proteins/genetics , Genetic Association Studies , Genotype , Humans , Lung Neoplasms/pathology , X-ray Repair Cross Complementing Protein 1 , Xeroderma Pigmentosum Group D Protein/geneticsABSTRACT
The results of the investigation of the interrelationship between frequency of chromosomal aberrations and detoxification enzymes (GSTM1, GSTT1) and DNA repair (hOGG1, XPD) genes in the employees of fuel energy complex in Kemerovo are presented In the group of the workers frequency of metaphases with aberrations (3,9 +/- 0,2%: n = 288) was shown to be significantly higher than in the comparison group (2,1 0, 2%: n = +/- 141). In the group of workers and control donors statistically significant differences were revealed in the frequency of distribution of the GSTT1 and hOGG1 genes. The level of chromosomal aberrations was established to be higher in patients with GSTM1 genotype "0/0" in the group of control donors.
Subject(s)
Air Pollutants, Occupational/toxicity , Chromosome Aberrations/chemically induced , DNA Repair Enzymes/genetics , Polymorphism, Genetic , Xenobiotics/toxicity , Adult , Air Pollutants, Occupational/pharmacokinetics , Cells, Cultured , Chromosome Aberrations/statistics & numerical data , DNA Glycosylases/genetics , Glutathione Transferase/genetics , Humans , Inactivation, Metabolic , Power Plants/standards , Real-Time Polymerase Chain Reaction , Xenobiotics/pharmacokinetics , Xeroderma Pigmentosum Group D Protein/geneticsABSTRACT
The paper presents the results of investigating an association between the frequency of chromosome aberrations and the CYP1A1, CYP1A2*1F GSTM1, and GSTT1 genes involved in xenobiotic biotransformation in workers engaged in heat and power engineering. The group of workers is shown to have a significantly higher rate of metaphases with aberrations (4.03 +/- 0.17%; n = 231) than the control group (2.06 +/- 0.17%; n = 141). Cytogenetic disorders are not found to depend on gender, age, and length of service. The study indicated that the level of chromosome aberrations was significantly higher in carriers of the CTP1A1 C/C, GSTM1 0/0 and GSTT1 0/0 genotypes. In individuals with a combination of the GSTM1 0/0 and GSTT1 0/0 genotypes, the level of chromosome aberrations was ascertained to be significantly higher than that in those with the protective form of these genes.
Subject(s)
Chromosome Aberrations , Genome, Human , Polymorphism, Genetic , Power Plants , Workplace/standards , Adult , Biotransformation/genetics , Case-Control Studies , Chromosome Aberrations/chemically induced , Chromosome Aberrations/statistics & numerical data , Data Interpretation, Statistical , Genotype , Humans , Mutagens/pharmacokinetics , Mutagens/toxicity , Occupational Exposure/adverse effects , Xenobiotics/pharmacokinetics , Xenobiotics/toxicityABSTRACT
Mutagenic and carcinogenic effects were studied in the population of Gornaya Shoria, Kemerovo Region. The carcinogenic effects were evaluated on the basis of the data given by the Kemerovo regional cancer registry over 1990-2008. The standardized cancer morbidity index in Gornaya Shoria (342 per 100,000 population) exceeded the average index in the Kemerovo Region (286 per 100,000) in the same period. The mutagenic effects were estimated on the basis of the monitoring data on the frequency and spectrum of chromosomal aberrations in the peripheral blood lymphocytes of the dwellers of Gornaya Shoria for the 1992-2009 period. The mean level of chromosomal aberrations (5.31%) in the residents of Gornaya Shoria was found to be greater than the regional background mutation rate (2.86%) (p < 0.001). The values of individual aberration types (chromatid and chromosomal breaks, as well as chromosome-type exchange aberrations) were significantly higher in the dwellers of Gornaya Shoria than those in the basic control group. The revealed high mutagenic load in the inhabitants of Tashtagol District, Kemerovo Region, allows this area to be assigned to high genetic risk ones.
