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Genetika ; 51(8): 895-904, 2015 Aug.
Article in Russian | MEDLINE | ID: mdl-26601489

ABSTRACT

A search was conducted for the alleles responsible for the quality of food-grade rapeseed oil in a collection of 21 samples of spring and winter oilseed rape of Belarusian and Russian breeding. We also developed A- and C-gene-specific DNA markers to assess the genomic polymorphisms of rape for FAD3 genes and selected plants with a low content of linolenic acid for use in the selection process. The development of a method for identifying FAD3 alleles, which control the level of linolenic acid in rapeseed oil, as well as of the design for new dCAPS markers, enabled the identification of plants homozygous for individual FAD3A and/or FAD3C genes in the F2-generation. These plants are currently involved in the selection process of new varieties with a reduced content of linolenic acid in rapeseed oil.


Subject(s)
Brassica napus/genetics , Fatty Acid Desaturases/genetics , Genetic Markers/genetics , Alleles , Breeding , Chromosome Mapping , Crosses, Genetic , Genome, Plant , Polymorphism, Genetic , Quantitative Trait Loci/genetics
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