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1.
Virology ; 306(2): 280-8, 2003 Feb 15.
Article in English | MEDLINE | ID: mdl-12642101

ABSTRACT

The X-ray crystal structure of Cowpea chlorotic mottle bromovirus (CCMV) revealed a unique tubular structure formed by the interaction of the N-termini from six coat protein subunits at each three-fold axis of the assembled virion. This structure, termed the beta-hexamer, consists of six short beta-strands. The beta-hexamer was postulated to play a critical role in the assembly and stability of the virion by stabilizing hexameric capsomers. Mutational analyses of the beta-hexamer structure, utilizing both in vitro and in vivo assembly assays, demonstrate that this structure is not required for virion formation devoid of nucleic acids in vitro or for RNA-containing virions in vivo. However, the beta-hexamer structure does contribute to virion stability in vitro and modulates disease expression in vivo. These results support a model for CCMV assembly through pentamer intermediates.


Subject(s)
Bromovirus/chemistry , Bromovirus/growth & development , Capsid Proteins/chemistry , Base Sequence , Bromovirus/genetics , Bromovirus/ultrastructure , Capsid Proteins/genetics , Capsid Proteins/ultrastructure , Cryoelectron Microscopy , Crystallography, X-Ray , DNA, Viral/genetics , Escherichia coli/genetics , Fabaceae/virology , Image Processing, Computer-Assisted , Models, Molecular , Plant Diseases/virology , Protein Structure, Secondary , Protein Subunits , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/ultrastructure , Sequence Deletion
2.
Nat Struct Biol ; 8(10): 874-8, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11573093

ABSTRACT

Group B coxsackieviruses (CVB) utilize the coxsackievirus-adenovirus receptor (CAR) to recognize host cells. CAR is a membrane protein with two Ig-like extracellular domains (D1 and D2), a transmembrane domain and a cytoplasmic domain. The three-dimensional structure of coxsackievirus B3 (CVB3) in complex with full length human CAR and also with the D1D2 fragment of CAR were determined to approximately 22 A resolution using cryo-electron microscopy (cryo-EM). Pairs of transmembrane domains of CAR associate with each other in a detergent cloud that mimics a cellular plasma membrane. This is the first view of a virus-receptor interaction at this resolution that includes the transmembrane and cytoplasmic portion of the receptor. CAR binds with the distal end of domain D1 in the canyon of CVB3, similar to how other receptor molecules bind to entero- and rhinoviruses. The previously described interface of CAR with the adenovirus knob protein utilizes a side surface of D1.


Subject(s)
Adenoviridae/metabolism , Enterovirus B, Human/metabolism , Receptors, Virus/metabolism , Adenoviridae/chemistry , HeLa Cells , Humans , Microscopy, Electron/methods , Models, Molecular , Receptors, Virus/chemistry , Viral Plaque Assay
3.
J Struct Biol ; 135(1): 38-46, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11562164

ABSTRACT

A method has been developed for three-dimensional image reconstruction of symmetry-mismatched components in tailed phages. Although the method described here addresses the specific case where differing symmetry axes are coincident, the method is more generally applicable, for instance, to the reconstruction of images of viral particles that deviate from icosahedral symmetry. Particles are initially oriented according to their dominant symmetry, thus reducing the search space for determining the orientation of the less dominant, symmetry-mismatched component. This procedure produced an improved reconstruction of the sixfold-symmetric tail assembly that is attached to the fivefold-symmetric prolate head of phi29, demonstrating that this method is capable of detecting and reconstructing an object that included a symmetry mismatch. A reconstruction of phi29 prohead particles using the methods described here establishes that the pRNA molecule has fivefold symmetry when attached to the prohead, consistent with its proposed role as a component of the stator in the phi29 DNA packaging motor.


Subject(s)
Bacillus Phages/chemistry , Imaging, Three-Dimensional/methods , Viral Structural Proteins/chemistry , Virion/chemistry , Bacillus Phages/ultrastructure , Cryoelectron Microscopy/methods , Fourier Analysis , Frozen Sections , Models, Structural , Rotation , Virion/ultrastructure , Virus Assembly
4.
J Virol ; 75(11): 5335-42, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11333914

ABSTRACT

Mammalian reoviruses, prototype members of the Reoviridae family of nonenveloped double-stranded RNA viruses, use at least three proteins--sigma1, mu1, and sigma3--to enter host cells. sigma1, a major determinant of cell tropism, mediates viral attachment to cellular receptors. Studies of sigma1 functions in reovirus entry have been restricted by the lack of methodologies to produce infectious virions containing engineered mutations in viral proteins. To mitigate this problem, we produced virion-like particles by "recoating" genome-containing core particles that lacked sigma1, mu1, and sigma3 with recombinant forms of these proteins in vitro. Image reconstructions from cryoelectron micrographs of the recoated particles revealed that they closely resembled native virions in three-dimensional structure, including features attributable to sigma1. The recoated particles bound to and infected cultured cells in a sigma1-dependent manner and were approximately 1 million times as infectious as cores and 0.5 times as infectious as native virions. Experiments with recoated particles containing recombinant sigma1 from either of two different reovirus strains confirmed that differences in cell attachment and infectivity previously observed between those strains are determined by the sigma1 protein. Additional experiments showed that recoated particles containing sigma1 proteins with engineered mutations can be used to analyze the effects of such mutations on the roles of particle-bound sigma1 in infection. The results demonstrate a powerful new system for molecular genetic dissections of sigma1 with respect to its structure, assembly into particles, and roles in entry.


Subject(s)
Capsid Proteins , Capsid/biosynthesis , Reoviridae/pathogenicity , Viral Proteins/genetics , Virus Replication , Baculoviridae , Capsid/genetics , Capsid/ultrastructure , Cell Line , Cryoelectron Microscopy , Eukaryotic Cells/virology , Hemagglutination Tests , Hemagglutinins, Viral/biosynthesis , Hemagglutinins, Viral/genetics , In Vitro Techniques , Microscopy, Electron , Recombinant Proteins/biosynthesis , Reoviridae/genetics , Reoviridae/ultrastructure , Viral Proteins/biosynthesis , Virus Assembly
5.
J Virol ; 75(5): 2444-51, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11160747

ABSTRACT

Coxsackievirus A21 (CAV21), like human rhinoviruses (HRVs), is a causative agent of the common cold. It uses the same cellular receptor, intercellular adhesion molecule 1 (ICAM-1), as does the major group of HRVs; unlike HRVs, however, it is stable at acid pH. The cryoelectron microscopy (cryoEM) image reconstruction of CAV21 is consistent with the highly homologous crystal structure of poliovirus 1; like other enteroviruses and HRVs, CAV21 has a canyon-like depression around each of the 12 fivefold vertices. A cryoEM reconstruction of CAV21 complexed with ICAM-1 shows all five domains of the extracellular component of ICAM-1. The known atomic structure of the ICAM-1 amino-terminal domains D1 and D2 has been fitted into the cryoEM density of the complex. The site of ICAM-1 binding within the canyon of CAV21 overlaps the site of receptor recognition utilized by rhinoviruses and polioviruses. Interactions within this common region may be essential for triggering viral destabilization after attachment to susceptible cells.


Subject(s)
Enterovirus/metabolism , Intercellular Adhesion Molecule-1/metabolism , Receptors, Virus/metabolism , Amino Acid Sequence , Binding Sites , Cryoelectron Microscopy , Enterovirus/chemistry , Humans , Image Processing, Computer-Assisted , Intercellular Adhesion Molecule-1/chemistry , Models, Molecular , Molecular Sequence Data , Protein Conformation , Protein Structure, Tertiary , Receptors, Virus/chemistry
6.
Virology ; 279(2): 385-91, 2001 Jan 20.
Article in English | MEDLINE | ID: mdl-11162794

ABSTRACT

The three-dimensional structure of DNA-filled, bacteriophage T4 isometric capsids has been determined by means of cryoelectron microscopy and image reconstruction techniques. The packing geometry of protein subunits on the capsid surface was confirmed to be that of the triangulation class T = 13. The reconstruction clearly shows pentamers, attributed to capsid protein gp24*, surrounded by hexamers of the major capsid protein, gp23*. Positions of the accessory proteins, Hoc and Soc, are also clearly delineated in the surface lattice. The Hoc protein is the most prominent surface feature and appears as an extended molecule with a rounded base from which a thin neck and a globular head protrude. One Hoc molecule associates with each hexamer. Nearly continuous "ridges" are formed at the periphery of the gp23* hexamers by an association of 12 Soc molecules; however, Soc is absent along the boundaries between the hexamers and the pentamers. The duplex DNA genome forms a highly condensed series of concentric layers, spaced about 2.36 nm apart, that follow the general contour of the inner wall of the protein capsid.


Subject(s)
Bacteriophage T4/ultrastructure , Capsid/ultrastructure , Cryoelectron Microscopy , Image Processing, Computer-Assisted , Models, Molecular , Models, Structural
7.
Virology ; 279(2): 471-7, 2001 Jan 20.
Article in English | MEDLINE | ID: mdl-11162803

ABSTRACT

The Geminiviridae is an extensive family of plant viruses responsible for economically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinned or "geminate" particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle, the Nigerian strain of Maize streak virus (MSV-N), using cryo-electron microscopy and three-dimensional image reconstruction methods. The particle, of dimensions 220 x 380 A, has an overall 52-point-group symmetry, in which each half particle "head" consists of the coat protein (CP) arranged with quasi-icosahedral symmetry. We have modeled the MSV-N CP as an eight-stranded, antiparallel beta-barrel motif (a structural motif common to all known ssDNA viruses) with an N-terminal alpha-helix. This has produced a model of the geminate particle in which 110 copies of the CP nicely fit into the reconstructed density map. The reconstructed density map and MSV-N pseudo-atomic model demonstrate that the geminate particle has a stable, defined structure.


Subject(s)
Capsid/chemistry , Geminiviridae/chemistry , Zea mays/virology , Amino Acid Sequence , Capsid/ultrastructure , Cryoelectron Microscopy , Geminiviridae/ultrastructure , Image Processing, Computer-Assisted , Models, Structural , Molecular Sequence Data , Protein Structure, Secondary , Sequence Alignment
8.
Dev Biol (Basel) ; 105: 111-22; discussion 149-50, 2001.
Article in English | MEDLINE | ID: mdl-11763320

ABSTRACT

C-Cluster enteroviruses (C-CEVs), consisting of Coxsackie A viruses (C-CAV1, 11, 13, 15, 17, 18, 19, 20, 21, 22, 24, 24v) and polioviruses (PV1, 2, 3), have been grouped together in relation to their genomic sequences. On the basis of disease syndromes caused in humans, however, C-CAVs and PVs are vastly different: the former cause respiratory disease, just like the major receptor group rhinoviruses (magHRV), whereas PVs, on invasion of the CNS, can cause poliomyelitis. It is assumed that the difference in pathogenesis of C-CEVs is governed predominantly by cellular receptor specificity. C-CAVs use ICAM-1, just like magHRV, whereas PVs uniquely use CD155. Both ICAM-1 and CD155 are Ig-like molecules. Remarkably, based on a phylogenetic analysis of non-structural proteins, CAV 11, 13, 17 and 18 are interleaved with, rather than separated from, the three PV serotypes, e.g. PV1 is more closely related to CAV18 that to PV2. This observation suggests that PVs may have emerged from a pool of C-CAVs by evolving a unique receptor specificity. We have been studying virion structure, virion/receptor interactions, genetics, and the molecular biology of C-CEVs with the objective of identifying the molecular basis of phenotypic diversity of these viruses. Of particular interest is the prospect that C-CEVs can be genetically manipulated to switch their receptor affinity: from CD155 to ICAM-1 for PVs, or from ICAM-1 to CD155 for C-CAVs. We propose a hypothesis that in a world free of poliovirus and anti-poliovirus neutralizing antibodies C-CAVs would be given a greater chance to switch receptor specificity from ICAM-1 to CD155 and thus, to evolve gradually into a new polio-like virus.


Subject(s)
Poliomyelitis/virology , Poliovirus/physiology , Animals , Evolution, Molecular , Humans , Models, Biological , Phylogeny , Picornaviridae/classification , Picornaviridae/genetics , Poliovirus/classification , Poliovirus/genetics , Poliovirus/pathogenicity , Receptors, Virus/genetics , Receptors, Virus/metabolism , Virion/physiology
9.
Nature ; 408(6813): 745-50, 2000 Dec 07.
Article in English | MEDLINE | ID: mdl-11130079

ABSTRACT

Motors generating mechanical force, powered by the hydrolysis of ATP, translocate double-stranded DNA into preformed capsids (proheads) of bacterial viruses and certain animal viruses. Here we describe the motor that packages the double-stranded DNA of the Bacillus subtilis bacteriophage phi29 into a precursor capsid. We determined the structure of the head-tail connector--the central component of the phi29 DNA packaging motor--to 3.2 A resolution by means of X-ray crystallography. We then fitted the connector into the electron densities of the prohead and of the partially packaged prohead as determined using cryo-electron microscopy and image reconstruction analysis. Our results suggest that the prohead plus dodecameric connector, prohead RNA, viral ATPase and DNA comprise a rotary motor with the head-prohead RNA-ATPase complex acting as a stator, the DNA acting as a spindle, and the connector as a ball-race. The helical nature of the DNA converts the rotary action of the connector into translation of the DNA.


Subject(s)
Bacillus Phages/chemistry , DNA, Viral/chemistry , Molecular Motor Proteins/chemistry , Adenosine Triphosphatases/chemistry , Bacillus Phages/genetics , Bacillus Phages/metabolism , Capsid/chemistry , Capsid/metabolism , Cryoelectron Microscopy , Crystallography, X-Ray , DNA, Viral/metabolism , Models, Molecular , Molecular Motor Proteins/metabolism , Nucleic Acid Conformation , RNA, Viral/chemistry
11.
Proc Natl Acad Sci U S A ; 97(1): 79-84, 2000 Jan 04.
Article in English | MEDLINE | ID: mdl-10618374

ABSTRACT

The structure of the extracellular, three-domain poliovirus receptor (CD155) complexed with poliovirus (serotype 1) has been determined to 22-A resolution by means of cryo-electron microscopy and three-dimensional image-reconstruction techniques. Density corresponding to the receptor was isolated in a difference electron density map and fitted with known structures, homologous to those of the three individual CD155 Ig-like domains. The fit was confirmed by the location of carbohydrate moieties in the CD155 glycoprotein, the conserved properties of elbow angles in the structures of cell surface molecules with Ig-like folds, and the concordance with prior results of CD155 and poliovirus mutagenesis. CD155 binds in the poliovirus "canyon" and has a footprint similar to that of the intercellular adhesion molecule-1 receptor on human rhinoviruses. However, the orientation of the long, slender CD155 molecule relative to the poliovirus surface is quite different from the orientation of intercellular adhesion molecule-1 on rhinoviruses. In addition, the residues that provide specificity of recognition differ for the two receptors. The principal feature of receptor binding common to these two picornaviruses is the site in the canyon at which binding occurs. This site may be a trigger for initiation of the subsequent uncoating step required for viral infection.


Subject(s)
Membrane Proteins , Poliovirus/chemistry , Receptors, Virus/chemistry , Amino Acid Sequence , Cryoelectron Microscopy , Glycosylation , Humans , Image Processing, Computer-Assisted , Models, Molecular , Poliovirus/ultrastructure , Receptors, Virus/ultrastructure , Sequence Alignment
13.
Microbiol Mol Biol Rev ; 63(4): 862-922, table of contents, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10585969

ABSTRACT

Viruses are cellular parasites. The linkage between viral and host functions makes the study of a viral life cycle an important key to cellular functions. A deeper understanding of many aspects of viral life cycles has emerged from coordinated molecular and structural studies carried out with a wide range of viral pathogens. Structural studies of viruses by means of cryo-electron microscopy and three-dimensional image reconstruction methods have grown explosively in the last decade. Here we review the use of cryo-electron microscopy for the determination of the structures of a number of icosahedral viruses. These studies span more than 20 virus families. Representative examples illustrate the use of moderate- to low-resolution (7- to 35-A) structural analyses to illuminate functional aspects of viral life cycles including host recognition, viral attachment, entry, genome release, viral transcription, translation, proassembly, maturation, release, and transmission, as well as mechanisms of host defense. The success of cryo-electron microscopy in combination with three-dimensional image reconstruction for icosahedral viruses provides a firm foundation for future explorations of more-complex viral pathogens, including the vast number that are nonspherical or nonsymmetrical.


Subject(s)
Cryoelectron Microscopy/methods , Image Processing, Computer-Assisted/methods , Viruses/ultrastructure , Crystallography, X-Ray , Models, Molecular , Models, Structural , Reproducibility of Results , Viruses/growth & development
14.
EMBO J ; 18(22): 6249-59, 1999 Nov 15.
Article in English | MEDLINE | ID: mdl-10562537

ABSTRACT

Two human rhinovirus serotypes complexed with two- and five-domain soluble fragments of the cellular receptor, intercellular adhesion molecule-1, have been investigated by X-ray crystallographic analyses of the individual components and by cryo-electron microscopy of the complexes. The three-dimensional image reconstructions provide a molecular envelope within which the crystal structures of the viruses and the receptor fragments can be positioned with accuracy. The N-terminal domain of the receptor binds to the rhinovirus 'canyon' surrounding the icosahedral 5-fold axes. Fitting of molecular models into the image reconstruction density identified the residues on the virus that interact with those on the receptor surface, demonstrating complementarity of the electrostatic patterns for the tip of the N-terminal receptor domain and the floor of the canyon. The complexes seen in the image reconstructions probably represent the first stage of a multistep binding process. A mechanism is proposed for the subsequent viral uncoating process.


Subject(s)
Intercellular Adhesion Molecule-1/chemistry , Intercellular Adhesion Molecule-1/ultrastructure , Receptors, Virus/chemistry , Receptors, Virus/ultrastructure , Rhinovirus/chemistry , Rhinovirus/ultrastructure , Binding Sites , Computer Graphics , Cryoelectron Microscopy , Crystallography, X-Ray , Humans , Intercellular Adhesion Molecule-1/physiology , Models, Molecular , Molecular Sequence Data , Protein Structure, Secondary , Receptors, Virus/physiology , Rhinovirus/physiology , Serotyping , Software , Static Electricity
15.
Proc Natl Acad Sci U S A ; 96(24): 13650-5, 1999 Nov 23.
Article in English | MEDLINE | ID: mdl-10570127

ABSTRACT

Repeated, specific interactions between capsid protein (CP) subunits direct virus capsid assembly and exemplify regulated protein-protein interactions. The results presented here reveal a striking in vivo switch in CP assembly. Using cryoelectron microscopy, three-dimensional image reconstruction, and molecular modeling, we show that brome mosaic virus (BMV) CP can assemble in vivo two remarkably distinct capsids that selectively package BMV-derived RNAs in the absence of BMV RNA replication: a 180-subunit capsid indistinguishable from virions produced in natural infections and a previously unobserved BMV capsid type with 120 subunits arranged as 60 CP dimers. Each such dimer contains two CPs in distinct, nonequivalent environments, in contrast to the quasi-equivalent CP environments throughout the 180-subunit capsid. This 120-subunit capsid utilizes most of the CP interactions of the 180-subunit capsid plus nonequivalent CP-CP interactions. Thus, the CP of BMV, and perhaps other viruses, can encode CP-CP interactions that are not apparent from mature virions and may function in assembly or disassembly. Shared structural features suggest that the 120- and 180-subunit capsids share assembly steps and that a common pentamer of CP dimers may be an important assembly intermediate. The ability of a single CP to switch between distinct capsids by means of alternate interactions also implies reduced evolutionary barriers between different capsid structures. The in vivo switch between alternate BMV capsids is controlled by the RNA packaged: a natural BMV genomic RNA was packaged in 180-subunit capsids, whereas an engineered mRNA containing only the BMV CP gene was packaged in 120-subunit capsids. RNA features can thus direct the assembly of a ribonucleoprotein complex between alternate structural pathways.


Subject(s)
Bromovirus/physiology , Capsid/physiology , Polymorphism, Genetic , RNA, Viral , Virus Assembly , Capsid/chemistry , Capsid/genetics , Models, Molecular , Protein Conformation , Saccharomyces cerevisiae , Virion
16.
J Virol ; 73(8): 6882-91, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10400786

ABSTRACT

The three-dimensional structure of expressed VP2 capsids of Aleutian mink disease parvovirus strain G (ADVG-VP2) has been determined to 22 A resolution by cryo-electron microscopy and image reconstruction techniques. A structure-based sequence alignment of the VP2 capsid protein of canine parvovirus (CPV) provided a means to construct an atomic model of the ADVG-VP2 capsid. The ADVG-VP2 reconstruction reveals a capsid structure with a mean external radius of 128 A and several surface features similar to those found in human parvovirus B19 (B19), CPV, feline panleukopenia virus (FPV), and minute virus of mice (MVM). Dimple-like depressions occur at the icosahedral twofold axes, canyon-like regions encircle the fivefold axes, and spike-like protrusions decorate the threefold axes. These spikes are not present in B19, and they are more prominent in ADV compared to the other parvoviruses owing to the presence of loop insertions which create mounds near the threefold axes. Cylindrical channels along the fivefold axes of CPV, FPV, and MVM, which are surrounded by five symmetry-related beta-ribbons, are closed in ADVG-VP2 and B19. Immunoreactive peptides made from segments of the ADVG-VP2 capsid protein map to residues in the mound structures. In vitro tissue tropism and in vivo pathogenic properties of ADV map to residues at the threefold axes and to the wall of the dimples.


Subject(s)
Aleutian Mink Disease Virus/chemistry , Aleutian Mink Disease/virology , Capsid/chemistry , Protein Structure, Secondary , Aleutian Mink Disease/pathology , Aleutian Mink Disease Virus/pathogenicity , Aleutian Mink Disease Virus/ultrastructure , Amino Acid Sequence , Animals , Capsid/ultrastructure , Capsid Proteins , Cats , Cell Line , Cryoelectron Microscopy , Dogs , Humans , Mice , Models, Molecular , Molecular Sequence Data , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/ultrastructure , Sequence Homology, Amino Acid , Spodoptera/cytology
17.
J Struct Biol ; 125(2-3): 166-75, 1999.
Article in English | MEDLINE | ID: mdl-10222272

ABSTRACT

Atomic-resolution structures have had a tremendous impact on modern biological science. Much useful information also has been gleaned by merging and correlating atomic-resolution structural details with lower-resolution (15-40 A), three-dimensional (3D) reconstructions computed from images recorded with cryo-transmission electron microscopy (cryoTEM) procedures. One way to merge these structures involves reducing the resolution of an atomic model to a level comparable to a cryoTEM reconstruction. A low-resolution density map can be derived from an atomic-resolution structure by retrieving a set of atomic coordinates editing the coordinate file, computing structure factors from the model coordinates, and computing the inverse Fourier transform of the structure factors. This method is a useful tool for structural studies primarily in combination with 3D cryoTEM reconstructions. It has been used to assess the quality of 3D reconstructions, to determine corrections for the phase-contrast transfer function of the transmission electron microscope, to calibrate the dimensions and handedness of 3D reconstructions, to produce difference maps, to model features in macromolecules or macromolecular complexes, and to generate models to initiate model-based determination of particle orientation and origin parameters for 3D reconstruction.


Subject(s)
Image Processing, Computer-Assisted , Models, Molecular , Molecular Structure , Algorithms , Calibration , Computer Graphics , Cryoelectron Microscopy , Crystallography, X-Ray , Fourier Analysis , Magnetic Resonance Spectroscopy , Microscopy, Electron , Microscopy, Phase-Contrast , Viruses/ultrastructure
18.
J Struct Biol ; 125(2-3): 246-52, 1999.
Article in English | MEDLINE | ID: mdl-10222281

ABSTRACT

We have developed a centralized World Wide Web (WWW)-based environment that serves as a resource of software tools and expertise for biological electron microscopy. A major focus is molecular electron microscopy, but the site also includes information and links on structural biology at all levels of resolution. This site serves to help integrate or link structural biology techniques in accordance with user needs. The WWW site, called the Electron Microscopy (EM) Outreach Program (URL: http://emoutreach.sdsc.edu), provides scientists with computational and educational tools for their research and edification. In particular, we have set up a centralized resource containing course notes, references, and links to image analysis and three-dimensional reconstruction software for investigators wanting to learn about EM techniques either within or outside of their fields of expertise.


Subject(s)
Internet , Microscopy, Electron , Academies and Institutes , California , Educational Technology , Forecasting , Image Processing, Computer-Assisted , National Institutes of Health (U.S.) , Research , Software , United States
19.
J Virol ; 73(5): 3941-50, 1999 May.
Article in English | MEDLINE | ID: mdl-10196289

ABSTRACT

Reovirus outer-capsid proteins mu1, sigma3, and sigma1 are thought to be assembled onto nascent core-like particles within infected cells, leading to the production of progeny virions. Consistent with this model, we report the in vitro assembly of baculovirus-expressed mu1 and sigma3 onto purified cores that lack mu1, sigma3, and sigma1. The resulting particles (recoated cores, or r-cores) closely resembled native virions in protein composition (except for lacking cell attachment protein sigma1), buoyant density, and particle morphology by scanning cryoelectron microscopy. Transmission cryoelectron microscopy and image reconstruction of r-cores confirmed that they closely resembled virions in the structure of the outer capsid and revealed that assembly of mu1 and sigma3 onto cores had induced rearrangement of the pentameric lambda2 turrets into a conformation approximating that in virions. r-cores, like virions, underwent proteolytic conversion to particles resembling native ISVPs (infectious subvirion particles) in protein composition, particle morphology, and capacity to permeabilize membranes in vitro. r-cores were 250- to 500-fold more infectious than cores in murine L cells and, like virions but not ISVPs or cores, were inhibited from productively infecting these cells by the presence of either NH4Cl or E-64. The latter results suggest that r-cores and virions used similar routes of entry into L cells, including processing by lysosomal cysteine proteinases, even though the former particles lacked the sigma1 protein. To examine the utility of r-cores for genetic dissections of mu1 functions in reovirus entry, we generated r-cores containing a mutant form of mu1 that had been engineered to resist cleavage at the delta:phi junction during conversion to ISVP-like particles by chymotrypsin in vitro. Despite their deficit in delta:phi cleavage, these ISVP-like particles were fully competent to permeabilize membranes in vitro and to infect L cells in the presence of NH4Cl, providing new evidence that this cleavage is dispensable for productive infection.


Subject(s)
Capsid Proteins , Capsid/metabolism , RNA-Binding Proteins , Reoviridae/physiology , Virus Assembly , Animals , Capsid/genetics , Chymotrypsin/metabolism , Cysteine Endopeptidases/metabolism , Erythrocytes , Hydrogen-Ion Concentration , Image Processing, Computer-Assisted , L Cells , Lysosomes , Mice , Microscopy, Electron , Microscopy, Electron, Scanning , Protein Conformation , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Reoviridae/ultrastructure , Virion/ultrastructure
20.
J Virol ; 73(4): 2963-73, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10074146

ABSTRACT

Structure-function studies with mammalian reoviruses have been limited by the lack of a reverse-genetic system for engineering mutations into the viral genome. To circumvent this limitation in a partial way for the major outer-capsid protein sigma3, we obtained in vitro assembly of large numbers of virion-like particles by binding baculovirus-expressed sigma3 protein to infectious subvirion particles (ISVPs) that lack sigma3. A level of sigma3 binding approaching 100% of that in native virions was routinely achieved. The sigma3 coat in these recoated ISVPs (rcISVPs) appeared very similar to that in virions by electron microscopy and three-dimensional image reconstruction. rcISVPs retained full infectivity in murine L cells, allowing their use to study sigma3 functions in virus entry. Upon infection, rcISVPs behaved identically to virions in showing an extended lag phase prior to exponential growth and in being inhibited from entering cells by either the weak base NH4Cl or the cysteine proteinase inhibitor E-64. rcISVPs also mimicked virions in being incapable of in vitro activation to mediate lysis of erythrocytes and transcription of the viral mRNAs. Last, rcISVPs behaved like virions in showing minor loss of infectivity at 52 degrees C. Since rcISVPs contain virion-like levels of sigma3 but contain outer-capsid protein mu1/mu1C mostly cleaved at the delta-phi junction as in ISVPs, the fact that rcISVPs behaved like virions (and not ISVPs) in all of the assays that we performed suggests that sigma3, and not the delta-phi cleavage of mu1/mu1C, determines the observed differences in behavior between virions and ISVPs. To demonstrate the applicability of rcISVPs for genetic studies of protein functions in reovirus entry (an approach that we call recoating genetics), we used chimeric sigma3 proteins to localize the primary determinants of a strain-dependent difference in sigma3 cleavage rate to a carboxy-terminal region of the ISVP-bound protein.


Subject(s)
Baculoviridae/physiology , Capsid Proteins , Capsid/physiology , RNA-Binding Proteins , Reoviridae Infections/virology , Reoviridae/physiology , Virion/physiology , Animals , Cell Line , DNA, Recombinant , Recombinant Fusion Proteins/physiology , Virus Replication
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