ABSTRACT
It was shown by the method of differential scanning microcalorimetry that an additional peak, at about 57 degrees C appears on heat absorption curves of the whole spleen tissue and cells of BalB/c mouse injected with Rausher virus. It is supposed that this peak is due to the melting of AT-rich regions of internucleosomal DNA in chromatin fibers of tumor cells, which results from the partial loss of proteins or the modification of H1 histone.
Subject(s)
Chromatin/chemistry , Leukemia, Experimental/metabolism , Spleen/metabolism , AT Rich Sequence , Animals , Calorimetry, Differential Scanning , DNA/metabolism , Leukemia, Experimental/pathology , Mice , Mice, Inbred BALB C , Protein Denaturation , Tumor Cells, CulturedSubject(s)
Plants/analysis , Water/analysis , Crystallization , Freezing , Magnetic Resonance SpectroscopySubject(s)
Plants/metabolism , Water/metabolism , Crystallization , Kinetics , Magnetic Resonance Spectroscopy , ThermodynamicsABSTRACT
Results of microcalorimetric studies of water crystallization in some plant tissues are presented. It was shown that in each tissue water is crystallized in a specific temperature region and the process is independent of water crystallization in other tissues. Besides this water fraction, there exists another one in the tissues which is united in a single system. The latter is crystallized simultaneously throughout the preparation. It is suggested that this water fraction is located in the tissues intercells. Results of secondary water crystallization in plant tissues are presented. It is shown that after freezing and thawing the core cells are damaged more strongly.