Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Inflamm Bowel Dis ; 18(5): 925-34, 2012 May.
Article in English | MEDLINE | ID: mdl-22114001

ABSTRACT

BACKGROUND: Chronic pouchitis is an important long-term complication following ileal pouch-anal anastomosis for ulcerative colitis. Antibiotic administration reduces symptoms of pouchitis, indicating that bacteria have a role in pathogenesis. The aim of the research was to investigate the bacterial content of pouches using nucleic acid-based methods. METHODS: Stool microbiota of 17 patients with normal pouches (NP), 17 patients with pouchitis (CP) utilizing samples collected from each patient when antibiotic-treated (CP-on, asymptomatic) and when untreated (CP-off, symptomatic), and 14 familial adenomatous polyposis (FAP) patients were analyzed by high-throughput sequencing, fluorescence in situ hybridization technologies, and quantitative polymerase chain reaction (qPCR). RESULTS: Fluorescence in situ hybridization analysis revealed an expanded phylogenetic gap in NP and CP-off patients relative to FAP. Antibiotic treatment reduced the gap in CP stool. The phylogenetic gap of CP-off patients was due to members of the bacterial families Caulobacteriaceae, Sphingomonadaceae, Comamonadaceae, Peptostreptococcaceae, and Clostridiaceae. There was a greater diversity of phylotypes of Clostridiaceae in CP-off subjects. The phylogenetic gap of NP stool was enriched by Ruminococcaceae and Bifidobacteriaceae. CP stool microbiota had reduced diversity relative to NP and FAP stool due largely to a reduction in Lachnospiraceae/Insertae Sedis XIV/clostridial cluster IV groups. CONCLUSIONS: Bacterial groups within the expanded phylogenetic gap of pouch patients may have roles in the pathogenesis of pouchitis. Further research concerning the physiology of cultured members of these groups will be necessary to explain their specific roles. Members of the Lachnospiraceae, Incertae Sedis XIV, and clostridial cluster IV could be useful biomarkers of pouch health.


Subject(s)
Adenomatous Polyposis Coli/microbiology , Bacteria/pathogenicity , Colitis, Ulcerative/microbiology , Colonic Pouches/microbiology , Feces/microbiology , Pouchitis/microbiology , Adenomatous Polyposis Coli/pathology , Adolescent , Adult , Child , Child, Preschool , Chronic Disease , Colitis, Ulcerative/pathology , Colonic Pouches/pathology , DNA/genetics , Female , High-Throughput Nucleotide Sequencing , Humans , In Situ Hybridization, Fluorescence , Infant , Infant, Newborn , Male , Pouchitis/pathology , Real-Time Polymerase Chain Reaction , Young Adult
SELECTION OF CITATIONS
SEARCH DETAIL
...