Subject(s)
Hypertension/physiopathology , Stress, Psychological/physiopathology , Adrenergic alpha-Agonists/pharmacology , Animals , Aorta, Abdominal/drug effects , Aorta, Abdominal/metabolism , Aorta, Abdominal/physiopathology , Arteries/drug effects , Arteries/physiopathology , Blood Pressure/drug effects , Hypertension/etiology , Hypertension/metabolism , In Vitro Techniques , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/metabolism , Muscle, Smooth, Vascular/physiopathology , Norepinephrine/pharmacology , Phosphatidylinositol Phosphates/metabolism , Rats , Rats, Wistar , Stress, Psychological/complications , Stress, Psychological/metabolism , Tail/blood supply , Vasoconstriction/drug effects , Vasoconstrictor Agents/pharmacologyABSTRACT
The presence of receptors for oligodeoxynucleotides (OdN) on the surface of L929 cells has previously been described. To study the possible coupling of the receptor to cellular signal transducing systems, the effect of phosphodiester OdN of different sequences on cellular phospholipase C and protein kinase C (PKC) activities in L929 fibroblasts was studied. Treatment of cells with OdN induced an increase in 32P labeling of phosphatidic acid which was accompanied by a gradual decrease in diacylglycerol. These effects seem to be independent of the OdN sequence. PKC activity in membranes isolated from OdN-treated cells was found to be lower than that in membranes of control cells. SDS-PAGE of the 32P-labeled cellular proteins revealed that OdN treatment caused a decrease in phosphorylation of the 26 and 73 kDa cellular proteins in the cells.
Subject(s)
Oligodeoxyribonucleotides/pharmacology , Protein Kinase C/metabolism , Type C Phospholipases/metabolism , Animals , Cell Line , Cell Membrane/enzymology , Cell Membrane/metabolism , Diglycerides/metabolism , Mice , Oligodeoxyribonucleotides/chemistry , Oligodeoxyribonucleotides/metabolism , Phosphatidic Acids/metabolism , Phosphorylation , Receptors, Cell Surface/metabolism , Signal TransductionABSTRACT
Oligo- and polynucleotides can not diffuse through lipid membrane, however they are taken up by eukaryotic cells by endocytosis mediated by the nucleic acid specific receptors. The compounds find some way to escape from endosomes and reach nucleic acids in both cell nucleus and cytoplasm. Oligonucleotides bind to a few cell surface proteins which take part in the virus-cell interaction and in the development of immune response. Interaction of nucleic acids with cell surface proteins may play a role in development of some pathologies. The biological role of this interaction is unclear. Efficient delivery of oligonucleotides into eukaryotic cells can be achieved in some conditions by natural mechanisms and by using artificial carriers--membrane vehicles and cationic polymer micelles.
Subject(s)
Cell Membrane/metabolism , Oligonucleotides/metabolism , Animals , Base Sequence , Biological Transport , Erythrocyte Membrane/metabolism , Humans , Membrane Proteins/metabolism , Molecular Sequence Data , Receptors, Cell Surface/metabolismABSTRACT
Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes.
