Semi-continuous anaerobic co-digestion of thermal and thermal-alkali processed organic fraction of municipal solid waste: Methane yield, energy analysis, anaerobic microbiome.

The semi-continuous anaerobic co-digestion (AcoD) of thermal and thermal-alkali pretreated organic fraction of municipal solid waste (OFMSW) and sewage sludge (SS) was studied under varying hydraulic retention times (HRT) and organic loading rates (OLR Three semi-continuous digesters were operated under control (non-pre-treated), thermally pretreated (125 °C), and thermal-alkali pretreated (125°C-3g/L NaOH) conditions at variable OLRs at 2.5, 4.0, 5.1, and 7.6 kgVS/m3.d and corresponding HRTs of 30, 20, 15, and 10 days. The 10 and 43% higher methane yield (0.445 m3/kgVS) and 11 and 57% higher VS removal (52%) was achieved for thermal-alkali pretreated digester at 5.1 kgVS/m3.d OLR over thermally pretreated (0.408 m3/kgVS, 45% VS removal) and control digesters (0.310 m3/kgVS, 33% VS removal), respectively. Thermal and thermal-alkali digesters failed on increasing the OLR to 7.6 kgVS/m3.d, whereas the control digester becomes upset at 5.1 kgVS/m3.d OLR. The metagenomic study revealed that Firmicutes, Bacteroidetes, Chloroflexi, Euryarchaeota, Proteobacteria, and Actinobacteria were the predominant bacterial population, whereas Methanosarcina and Methanothrix dominated the archaeal community. Energy balance analysis revealed that thermal alkali pretreatment showed the highest positive energy balance of 114.6 MJ/ton with an energy ratio of 1.25 compared with thermally pretreated (81.5 MJ/ton) and control samples (-46.9 MJ/ton). This work pave the way for scaleup of both thermal and thermal-alkali pre-treatment at 125 °C to realize the techno-economic and energy potential of the process.

Advanced steam-explosion pretreatment mediated anaerobic digestion of municipal sludge: Effects on methane yield, emerging contaminants removal, and microbial community.

Advanced steam explosion pretreatment, i.e., the Thermal hydrolysis process (THP) is applied mainly to improve the sludge solubilization and subsequent methane yield in the downstream anaerobic digestion (AD) process. However, the potential of THP in pretreating the high solids retention time (SRT) sludges, mitigating the risk of emerging organic micropollutants and effects on anaerobic microbiome in digester remains unclear. In this study, sludge from a sequencing batch reactor (SBR) system operating at a SRT of 40 days was subjected to THP using a 5 L pilot plant at the temperature ranges of 120-180 °C for 30-120 min. The effect of THP on organics solubilization, methane yield, organic micropollutant removal, and microbial community dynamics was studied. The highest methane yield of 507 mL CH4/g VSadded and volatile solids (VS) removal of 54% were observed at 160°C- 30min THP condition, i.e., 4.1 and 2.6 times higher than the control (123 mL CH4/gVSadded, 20.7%), respectively. The experimental values of hydrolysis coefficient and methane yield have been predicted using Modified Gompertz, First order, and Logistics models. The observed values fitted well with all three models showing an R2 value between 0.96 and 1.0. THP pretreated sludges showed >80% removal of Trimethoprim, Enrofloxacin, Ciprofloxacin, and Bezafibrate. However, Carbamazepine, 17α-ethinylestradiol, and Progesterone showed recalcitrant behavior, resulting in less than 50% removal. Microbial diversity analysis showed the dominance of Proteobacteria, Firmicutes, Chloroflexi, and Bacteroidetes, collectively accounting for >70-80% of bacterial reads. They are mainly responsible for the fermentation of complex biomolecules like polysaccharides, proteins, and lipids. The THP-mediated anaerobic digestion of sludge shows better performance than the control digestion, improved methane yield, higher VS and micropollutants removal, and a diverse microbiome in the digester.

Principles and potential of thermal hydrolysis of sewage sludge to enhance anaerobic digestion.

Sewage sludge is rich source of carbon, nutrients, and trace elements and can be subjected to proper treatment before disposal to fulfill government legislation and protect receiving environments. Anaerobic digestion (AD) is a well-adopted technology for stabilizing sewage sludge and recovering energy-rich biogas and nutrient-rich digestate. However, a slow hydrolysis rate limits the biodegradability of sludge. In the present study we have attempted to explain the potential of thermal hydrolysis to enhance anaerobic digestion of sewage sludge. Thermal pretreatment improves biodegradability and recycling of the sludge as an excellent energy and nutrients recovery source at reasonable capital (CAPEX) and operational (OPEX) costs. Other pretreatments like conventional (below/above 100 °C), temperature-phased anaerobic digestion (TPAD), microwave and chemically mediated thermal pretreatment have also been accounted. This review provides a holistic overview of sludge's characterization and value-added properties, various techniques used for sludge pretreatment for resource recovery, emphasizing conventional and advanced thermal pretreatment, challenges in scale-up of these technologies, and successful commercialization of thermal pretreatment techniques.

Energy feasibility and life cycle assessment of sludge pretreatment methods for advanced anaerobic digestion.

Energy sustainability is one of the critical parameters to be studied for the successful application of pretreatment processes. This study critically analyzes the energy efficiency of different energy-demanding sludge pretreatment techniques. Conventional thermal pretreatment of sludge (∼5% total solids, TS) produced 244 mL CH4/gTS, which could result in a positive energy balance of 2.6 kJ/kg TS. However, microwave pretreatment could generate only 178 mL CH4/gTS with a negative energy balance of -15.62 kJ/kg TS. In CAMBI process, the heat requirements can be compensated using exhaust gases and hot water from combined heat and power, and electricity requirements are managed by the use of cogeneration. The study concluded that <100 â„ƒ pretreatment effectively enhances the efficiency of anaerobic digestion and shows positive energy balance over microwave and ultrasonication. Moreover, microwave pretreatment has the highest global warming potential than thermal and ultrasonic pretreatments.

Recalcitrant compounds formation, their toxicity, and mitigation: Key issues in biomass pretreatment and anaerobic digestion.

Increasing energy demands and environmental issues have stressed the importance of sustainable methods of energy production. Anaerobic digestion (AD) of the biodegradable waste, i.e., agricultural residues, organic fraction of municipal solid waste (OFMSW), sewage sludge, etc., results in the production of biogas, which is a sustainable and cost feasible technique that reduces the dependence on fossil fuels and also overcomes the problems associated with biomass waste management. To solubilize the organic matter and enhance the susceptibility of hardly biodegradable fraction (i.e., lignocellulosic) for hydrolysis and increase methane production, several pretreatments, including physical, chemical, biological, and hybrid methods have been studied. However, these pretreatment methods under specific operating conditions result in the formation of recalcitrant compounds, such as sugars (xylose, Xylo-oligomers), organic acids (acetic, formic, levulinic acids), and lignin derivatives (poly and mono-phenolic compounds), causing significant inhibitory effects on anaerobic digestion. During the scaling up of these techniques from laboratory to industrial level, the focus on managing inhibitory compounds formed during pretreatment is envisaged to increase because of the need to use recalcitrant feedstocks in anaerobic digestion to increase biogas productivity. Therefore, it is crucial to understand the production mechanism of inhibitory compounds during pretreatment and work out the possible detoxification methods to improve anaerobic digestion. This paper critically reviews the earlier works based on the formation of recalcitrant compounds during feedstocks pretreatment under variable conditions, and their detrimental effects on process performance. The technologies to mitigate recalcitrant toxicity are also comprehensively discussed.