Integrating eco-technological approach for textile dye effluent treatment and carbon dioxide capturing from unicellular microalga Chlorella vulgaris RDS03: a synergistic method.

A pilot-scale treatment method was used in the present study to test the biosorption of textile dye from textile effluent and carbon dioxide using Chlorella vulgaris RDS03. The textile dye effluent treatment achieved that textile dye biosorption capacity (qmax) rate of 98.84% on 15 days of treatment using Chlorella vulgaris RDS03. The Langmuir and Freundlich isotherm kinetics model indicated that the higher R2 value 0.98. The microalga Chlorella vulgaris RDS03 was captured-96.86% of CO2 analyzed by CO2 utilization and biofixation kinetics, 4.65 mgmL-1 of biomass, 189.26 mgg-1 of carbohydrate, 233.89 mgg-1 of lipid, 4.3 mLg-1 of bioethanol and 4.9 mLg-1 of biodiesel produced. We performed fatty acid methyl ester (FAME) profiling using gas chromatography-mass spectrometry (GCMS). We found 40 types of biodiesel compounds, specifically myristic acid, pentadecanoic acid, octadecanoic acid, palmitic acid, and oleic acid. The high-performance liquid chromatography (HPLC) validated and analyzed the produced bioethanol.Novelty of the Research• Unicellular microalga Chlorella vulgaris RDS03 was isolated from the freshwater region and investigated their biosorption efficiency against hazardous synthetic azo textile dyes.• Chlorella vulgaris RDS03 ability to biosorption 96.86% of environmental polluted carbon dioxide• Treated biomass was used to produce ecofriendly, unpolluted and green energy such as biofuels (biodiesel and bioethanol).

Pilot scale wastewater treatment, CO2 sequestration and lipid production using microalga, Neochloris aquatica RDS02.

In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, Neochloris aquatica RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using N. aquatica RDS02. The microalgal biosorption capacity q max rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher R 2 value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO2 (90%) analyzed by CO2 biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL-1), carbohydrate (102.75 mg mL-1), biodiesel (4.9 mL g-1) and bioethanol (4.1 mL g-1). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling viz., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and oleic acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups viz., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze.