Piperacillin/Tazobactam in critically ill morbidly obese patients: A case series: The first One-Centre experience with TDM.

The aim of this article is to demonstrate extreme interindividual variability of piperacilin/tazobactam (PIP/TAZO) pharmacokinetics in critically ill morbidly obese patients and to emphasize the need for the practice of routine PIP/TAZO plasma concentrations measurement in order to ensure optimal efficacy and safety of antibiotic therapy.

[Technical problems in the preparation of biopsy samples of the spleen]. / Technické problémy spracovania bioptických vzoriek sleziny.

Spleen tissue sampling for bioptical examination should be performed after mutual consultation of clinician and pathologist. Clinician should know what is expected from the pathologist. This can influence preparation and sampling method considerably. In a great amount of cases the whole organ is removed. A lab technician is sampling on her own and her contribution to a good result can be either positive or negative. Imprint preparations from the tissue sections can be useful. Specialized examination should be performed in the presence of a pathologist or his co-workers. The active approach of a lab technician can significantly contribute to the value of examined sample of the spleen, of the organ until recently considered to be not important or full of mysteries.